Effect of gamma irradiation on the physical stability of DPPC liposomes

Unilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) were prepared by the reverse-phase evaporation method and extrusion through a polycarbonate membrane filter. Liposomes at 0.7 mg/mL lipid concentration in deionized water were exposed to gamma irradiation at a dose in the range...

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Main Authors:	Liyana Mohd Ali Napia, Irman Abdul Rahman, Mohd Yusof Hamzah, Faizal Mohamed, Hur Munawar Kabir Mohd, Intan Syakeela Ahmad Bastamam, Shamellia Sharin, Norsyahidah Mohd Hidzir, Shahidan Radiman
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12128/ http://journalarticle.ukm.my/12128/ http://journalarticle.ukm.my/12128/1/19%20Liyana%20Mohd%20Ali%20Napia.pdf

id	ukm-12128
recordtype	eprints
spelling	ukm-121282018-09-28T22:36:31Z http://journalarticle.ukm.my/12128/ Effect of gamma irradiation on the physical stability of DPPC liposomes Liyana Mohd Ali Napia, Irman Abdul Rahman, Mohd Yusof Hamzah, Faizal Mohamed, Hur Munawar Kabir Mohd, Intan Syakeela Ahmad Bastamam, Shamellia Sharin, Norsyahidah Mohd Hidzir, Shahidan Radiman, Unilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) were prepared by the reverse-phase evaporation method and extrusion through a polycarbonate membrane filter. Liposomes at 0.7 mg/mL lipid concentration in deionized water were exposed to gamma irradiation at a dose in the range 0.5 to 25 kGy. Gamma irradiation of liposomes resulted in the degradation of DPPC lipids into free fatty acids, lysophosphatidylcholine and 1,2-palmitoyl-phosphatidic acid (DPPA). The effect of gamma irradiation towards the physical stability of liposomes was investigated by means of dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential analysis. From the DLS analysis, no significant changes were observed in the hydrodynamic size of liposomes. TEM images indicate that the liposomes surface became smoother and rounder as higher irradiation doses were applied. Zeta potential analysis showed that gamma irradiation of DPPC liposomes at radiation doses as low as 0.5 kGy resulted in a drastic rise in the magnitude of the zeta potential. The results also demonstrate that gamma irradiation of liposomes suspension enhanced the overall stability of liposomes. Hence, it can be concluded that gamma irradiation on DPPC liposomes may potentially produce liposomes with higher stability. Penerbit Universiti Kebangsaan Malaysia 2018-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12128/1/19%20Liyana%20Mohd%20Ali%20Napia.pdf Liyana Mohd Ali Napia, and Irman Abdul Rahman, and Mohd Yusof Hamzah, and Faizal Mohamed, and Hur Munawar Kabir Mohd, and Intan Syakeela Ahmad Bastamam, and Shamellia Sharin, and Norsyahidah Mohd Hidzir, and Shahidan Radiman, (2018) Effect of gamma irradiation on the physical stability of DPPC liposomes. Sains Malaysiana, 47 (6). pp. 1235-1240. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil6_2018/KandunganJilid47Bil6_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Unilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) were prepared by the reverse-phase evaporation method and extrusion through a polycarbonate membrane filter. Liposomes at 0.7 mg/mL lipid concentration in deionized water were exposed to gamma irradiation at a dose in the range 0.5 to 25 kGy. Gamma irradiation of liposomes resulted in the degradation of DPPC lipids into free fatty acids, lysophosphatidylcholine and 1,2-palmitoyl-phosphatidic acid (DPPA). The effect of gamma irradiation towards the physical stability of liposomes was investigated by means of dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential analysis. From the DLS analysis, no significant changes were observed in the hydrodynamic size of liposomes. TEM images indicate that the liposomes surface became smoother and rounder as higher irradiation doses were applied. Zeta potential analysis showed that gamma irradiation of DPPC liposomes at radiation doses as low as 0.5 kGy resulted in a drastic rise in the magnitude of the zeta potential. The results also demonstrate that gamma irradiation of liposomes suspension enhanced the overall stability of liposomes. Hence, it can be concluded that gamma irradiation on DPPC liposomes may potentially produce liposomes with higher stability.
format	Article
author	Liyana Mohd Ali Napia, Irman Abdul Rahman, Mohd Yusof Hamzah, Faizal Mohamed, Hur Munawar Kabir Mohd, Intan Syakeela Ahmad Bastamam, Shamellia Sharin, Norsyahidah Mohd Hidzir, Shahidan Radiman,
spellingShingle	Liyana Mohd Ali Napia, Irman Abdul Rahman, Mohd Yusof Hamzah, Faizal Mohamed, Hur Munawar Kabir Mohd, Intan Syakeela Ahmad Bastamam, Shamellia Sharin, Norsyahidah Mohd Hidzir, Shahidan Radiman, Effect of gamma irradiation on the physical stability of DPPC liposomes
author_facet	Liyana Mohd Ali Napia, Irman Abdul Rahman, Mohd Yusof Hamzah, Faizal Mohamed, Hur Munawar Kabir Mohd, Intan Syakeela Ahmad Bastamam, Shamellia Sharin, Norsyahidah Mohd Hidzir, Shahidan Radiman,
author_sort	Liyana Mohd Ali Napia,
title	Effect of gamma irradiation on the physical stability of DPPC liposomes
title_short	Effect of gamma irradiation on the physical stability of DPPC liposomes
title_full	Effect of gamma irradiation on the physical stability of DPPC liposomes
title_fullStr	Effect of gamma irradiation on the physical stability of DPPC liposomes
title_full_unstemmed	Effect of gamma irradiation on the physical stability of DPPC liposomes
title_sort	effect of gamma irradiation on the physical stability of dppc liposomes
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12128/ http://journalarticle.ukm.my/12128/ http://journalarticle.ukm.my/12128/1/19%20Liyana%20Mohd%20Ali%20Napia.pdf
first_indexed	2023-09-18T20:01:55Z
last_indexed	2023-09-18T20:01:55Z
_version_	1777406905468059648

Effect of gamma irradiation on the physical stability of DPPC liposomes

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