Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina

Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina

Phalaenopsis bellina is an important indigenous fragrant orchid threatened with extinction. In this study, we evaluated the effect of medium strength, sucrose, nitrogen (NH4NO3) and potato extract on proliferation of P. bellina protocorm-like bodies (PLBs) to improve micropropagation in this species...

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Main Authors: Chew, Yee-Chern, Muhammad Husni Abd Halim, Wan Muhamad Asrul Nizam Wan Abdullah, Janna Ong Abdullah, Lai, Kok-Song
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12110/
http://journalarticle.ukm.my/12110/
http://journalarticle.ukm.my/12110/1/03%20Yee-Chern%20Chew.pdf
id ukm-12110
recordtype eprints
spelling ukm-121102018-09-28T21:53:12Z http://journalarticle.ukm.my/12110/ Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina Chew, Yee-Chern Muhammad Husni Abd Halim, Wan Muhamad Asrul Nizam Wan Abdullah, Janna Ong Abdullah, Lai, Kok-Song Phalaenopsis bellina is an important indigenous fragrant orchid threatened with extinction. In this study, we evaluated the effect of medium strength, sucrose, nitrogen (NH4NO3) and potato extract on proliferation of P. bellina protocorm-like bodies (PLBs) to improve micropropagation in this species. Optimal treatment for PLBs proliferation rate with an average fresh weight (FW) of 0.97±0.16 g was obtained through culturing on half strength (½) MS medium containing 20 g/L sucrose, 15 mM NH4NO3 and 20% w/v potato extract supplemented with 0.8 μM 2,4 dichlorophenoxyacetic acid (2,4-D). The optimal treatment produced large, healthy and greenish PLBs with reduction in the occurrence of culture browning. In contrast, treatments with high potato extract (>20% w/v) or NH4NO3 (>30 mM) concentrations tend to have inhibitory effect and resulted in low PLBs proliferation rate, with an average FW of 0.77±0.15 g and 0.69±0.15 g, respectively. Plant regeneration of PLBs was achieved on plant growth regulator (PGR)-free ½ MS medium. In total, 60 healthy PLBs from the optimal treatment were successfully regenerated, acclimatized with 100% survival percentage and grew well in a mixture of soil, sand and vermicompost (8:4:2 (w/w/w)). With the optimal treatment, PLBs proliferation rate was enhanced by 27.63%. Our findings offer an improved micropropagation protocol of the endangered P. bellina for conservation and commercial production. Penerbit Universiti Kebangsaan Malaysia 2018-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12110/1/03%20Yee-Chern%20Chew.pdf Chew, Yee-Chern and Muhammad Husni Abd Halim, and Wan Muhamad Asrul Nizam Wan Abdullah, and Janna Ong Abdullah, and Lai, Kok-Song (2018) Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina. Sains Malaysiana, 47 (6). pp. 1093-1099. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil6_2018/KandunganJilid47Bil6_2018.html
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language English
description Phalaenopsis bellina is an important indigenous fragrant orchid threatened with extinction. In this study, we evaluated the effect of medium strength, sucrose, nitrogen (NH4NO3) and potato extract on proliferation of P. bellina protocorm-like bodies (PLBs) to improve micropropagation in this species. Optimal treatment for PLBs proliferation rate with an average fresh weight (FW) of 0.97±0.16 g was obtained through culturing on half strength (½) MS medium containing 20 g/L sucrose, 15 mM NH4NO3 and 20% w/v potato extract supplemented with 0.8 μM 2,4 dichlorophenoxyacetic acid (2,4-D). The optimal treatment produced large, healthy and greenish PLBs with reduction in the occurrence of culture browning. In contrast, treatments with high potato extract (>20% w/v) or NH4NO3 (>30 mM) concentrations tend to have inhibitory effect and resulted in low PLBs proliferation rate, with an average FW of 0.77±0.15 g and 0.69±0.15 g, respectively. Plant regeneration of PLBs was achieved on plant growth regulator (PGR)-free ½ MS medium. In total, 60 healthy PLBs from the optimal treatment were successfully regenerated, acclimatized with 100% survival percentage and grew well in a mixture of soil, sand and vermicompost (8:4:2 (w/w/w)). With the optimal treatment, PLBs proliferation rate was enhanced by 27.63%. Our findings offer an improved micropropagation protocol of the endangered P. bellina for conservation and commercial production.
format Article
author Chew, Yee-Chern
Muhammad Husni Abd Halim,
Wan Muhamad Asrul Nizam Wan Abdullah,
Janna Ong Abdullah,
Lai, Kok-Song
spellingShingle Chew, Yee-Chern
Muhammad Husni Abd Halim,
Wan Muhamad Asrul Nizam Wan Abdullah,
Janna Ong Abdullah,
Lai, Kok-Song
Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina
author_facet Chew, Yee-Chern
Muhammad Husni Abd Halim,
Wan Muhamad Asrul Nizam Wan Abdullah,
Janna Ong Abdullah,
Lai, Kok-Song
author_sort Chew, Yee-Chern
title Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina
title_short Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina
title_full Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina
title_fullStr Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina
title_full_unstemmed Highly efficient proliferation and regeneration of protocorm-like bodies (PLBS) of the threatened orchid, Phalaenopsis bellina
title_sort highly efficient proliferation and regeneration of protocorm-like bodies (plbs) of the threatened orchid, phalaenopsis bellina
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12110/
http://journalarticle.ukm.my/12110/
http://journalarticle.ukm.my/12110/1/03%20Yee-Chern%20Chew.pdf
first_indexed 2023-09-18T20:01:53Z
last_indexed 2023-09-18T20:01:53Z
_version_ 1777406903123443712

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