Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process

Pulp and paper mill sludge (PPMS) was found to be poorly colonised with thermophilic microorganisms. However, evidence to support the need for inoculation to facilitate PPMS composting has only been demonstrated in one instance. In this study, we aimed to: screen and identify PPMS digesting thermoph...

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Main Authors:	Chau, Ngo Thi Tuong, Thien, Le Van, Lan, Pham Thi Ngoc, Futamata, Hiroyuki
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12096/ http://journalarticle.ukm.my/12096/ http://journalarticle.ukm.my/12096/1/23%20Ngo%20Thi%20Tuong%20Chau.pdf

id	ukm-12096
recordtype	eprints
spelling	ukm-120962018-09-22T10:50:54Z http://journalarticle.ukm.my/12096/ Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process Chau, Ngo Thi Tuong Thien, Le Van Lan, Pham Thi Ngoc Futamata, Hiroyuki Pulp and paper mill sludge (PPMS) was found to be poorly colonised with thermophilic microorganisms. However, evidence to support the need for inoculation to facilitate PPMS composting has only been demonstrated in one instance. In this study, we aimed to: screen and identify PPMS digesting thermophilic bacterial strains; investigate effects of the mixture of selected thermophilic bacterial strains on PPMS digestion; and utilize this mixture as start inoculum in PPMS composting and assess the quality of compost product. The results showed that eleven thermophilic bacterial strains were isolated from Bai Bang PPMS by the enrichment culture method. Among these, three strains which reflected high growth rates on the plates of Minimal Media Agar supplemented with Bai Bang PPMS and showed hydrolytic and ligninolytic activities on the agar plates containing appropriate inductive substrates were selected. Based on the morphological, biochemical characteristics and 16S rRNA gene sequencing, they were identified as Bacillus subtilis. The inoculation with the mixture of selected strains enhanced remarkably Bai Bang PPMS digestion. The dry weight decrease, volatile suspended solids removal, dehydrogenase and protease activities in the inoculated sludge were 2.1-, 1.5-, 1.3- and 1.2- fold higher, respectively, compared to the non-inoculated sludge. The assessment of compost quality based on stability using the alkaline trap method and maturity using the germination and root elongation test showed that the inoculated compost was stable and mature while the non-inoculated compost was unstable and immature. These thermophilic bacterial strains therefore have great potential for Bai Bang PPMS composting. Penerbit Universiti Kebangsaan Malaysia 2018-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12096/1/23%20Ngo%20Thi%20Tuong%20Chau.pdf Chau, Ngo Thi Tuong and Thien, Le Van and Lan, Pham Thi Ngoc and Futamata, Hiroyuki (2018) Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process. Sains Malaysiana, 47 (5). pp. 1051-1060. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num5_2018/contentsVol47num5_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Pulp and paper mill sludge (PPMS) was found to be poorly colonised with thermophilic microorganisms. However, evidence to support the need for inoculation to facilitate PPMS composting has only been demonstrated in one instance. In this study, we aimed to: screen and identify PPMS digesting thermophilic bacterial strains; investigate effects of the mixture of selected thermophilic bacterial strains on PPMS digestion; and utilize this mixture as start inoculum in PPMS composting and assess the quality of compost product. The results showed that eleven thermophilic bacterial strains were isolated from Bai Bang PPMS by the enrichment culture method. Among these, three strains which reflected high growth rates on the plates of Minimal Media Agar supplemented with Bai Bang PPMS and showed hydrolytic and ligninolytic activities on the agar plates containing appropriate inductive substrates were selected. Based on the morphological, biochemical characteristics and 16S rRNA gene sequencing, they were identified as Bacillus subtilis. The inoculation with the mixture of selected strains enhanced remarkably Bai Bang PPMS digestion. The dry weight decrease, volatile suspended solids removal, dehydrogenase and protease activities in the inoculated sludge were 2.1-, 1.5-, 1.3- and 1.2- fold higher, respectively, compared to the non-inoculated sludge. The assessment of compost quality based on stability using the alkaline trap method and maturity using the germination and root elongation test showed that the inoculated compost was stable and mature while the non-inoculated compost was unstable and immature. These thermophilic bacterial strains therefore have great potential for Bai Bang PPMS composting.
format	Article
author	Chau, Ngo Thi Tuong Thien, Le Van Lan, Pham Thi Ngoc Futamata, Hiroyuki
spellingShingle	Chau, Ngo Thi Tuong Thien, Le Van Lan, Pham Thi Ngoc Futamata, Hiroyuki Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process
author_facet	Chau, Ngo Thi Tuong Thien, Le Van Lan, Pham Thi Ngoc Futamata, Hiroyuki
author_sort	Chau, Ngo Thi Tuong
title	Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process
title_short	Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process
title_full	Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process
title_fullStr	Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process
title_full_unstemmed	Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process
title_sort	characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12096/ http://journalarticle.ukm.my/12096/ http://journalarticle.ukm.my/12096/1/23%20Ngo%20Thi%20Tuong%20Chau.pdf
first_indexed	2023-09-18T20:01:51Z
last_indexed	2023-09-18T20:01:51Z
_version_	1777406901087109120

Characterization and utilization of pulp and paper mill sludge digesting thermophilic bacteria in composting process

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