Surface modification of cellulose nanomaterial for urea biosensor application

Cellulose nanomaterial with rod-like structure and highly crystalline order, usually formed by elimination of the amorphous region from cellulose during acid hydrolysis. Cellulose nanomaterial with the property of biocompatibility and nontoxicity can be used for enzyme immobilization. In this work,...

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Main Authors:	Wan Elina Faradilla Wan Khalid, Lee, Yook Heng, Mohamad Nasir Mat Arip
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12082/ http://journalarticle.ukm.my/12082/ http://journalarticle.ukm.my/12082/1/09%20Wan%20Elina%20Faradilla.pdf

id	ukm-12082
recordtype	eprints
spelling	ukm-120822018-09-22T10:42:36Z http://journalarticle.ukm.my/12082/ Surface modification of cellulose nanomaterial for urea biosensor application Wan Elina Faradilla Wan Khalid, Lee, Yook Heng Mohamad Nasir Mat Arip, Cellulose nanomaterial with rod-like structure and highly crystalline order, usually formed by elimination of the amorphous region from cellulose during acid hydrolysis. Cellulose nanomaterial with the property of biocompatibility and nontoxicity can be used for enzyme immobilization. In this work, urease enzyme was used as a model enzyme to study the surface modification of cellulose nanomaterial and its potential for biosensor application. The cellulose nanocrystal (CNC) surface was modified using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation to introduce the carboxyl group at C6 primary alcohol. The success of enzyme immobilization and surface modification was confirmed using chemical tests and measured using UV-Visible spectrophotometer. The immobilization strategy was then applied for biosensor application for urea detection. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used for electroanalytical characterization of the urea biosensor. Penerbit Universiti Kebangsaan Malaysia 2018-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12082/1/09%20Wan%20Elina%20Faradilla.pdf Wan Elina Faradilla Wan Khalid, and Lee, Yook Heng and Mohamad Nasir Mat Arip, (2018) Surface modification of cellulose nanomaterial for urea biosensor application. Sains Malaysiana, 47 (5). pp. 941-949. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num5_2018/contentsVol47num5_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Cellulose nanomaterial with rod-like structure and highly crystalline order, usually formed by elimination of the amorphous region from cellulose during acid hydrolysis. Cellulose nanomaterial with the property of biocompatibility and nontoxicity can be used for enzyme immobilization. In this work, urease enzyme was used as a model enzyme to study the surface modification of cellulose nanomaterial and its potential for biosensor application. The cellulose nanocrystal (CNC) surface was modified using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation to introduce the carboxyl group at C6 primary alcohol. The success of enzyme immobilization and surface modification was confirmed using chemical tests and measured using UV-Visible spectrophotometer. The immobilization strategy was then applied for biosensor application for urea detection. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used for electroanalytical characterization of the urea biosensor.
format	Article
author	Wan Elina Faradilla Wan Khalid, Lee, Yook Heng Mohamad Nasir Mat Arip,
spellingShingle	Wan Elina Faradilla Wan Khalid, Lee, Yook Heng Mohamad Nasir Mat Arip, Surface modification of cellulose nanomaterial for urea biosensor application
author_facet	Wan Elina Faradilla Wan Khalid, Lee, Yook Heng Mohamad Nasir Mat Arip,
author_sort	Wan Elina Faradilla Wan Khalid,
title	Surface modification of cellulose nanomaterial for urea biosensor application
title_short	Surface modification of cellulose nanomaterial for urea biosensor application
title_full	Surface modification of cellulose nanomaterial for urea biosensor application
title_fullStr	Surface modification of cellulose nanomaterial for urea biosensor application
title_full_unstemmed	Surface modification of cellulose nanomaterial for urea biosensor application
title_sort	surface modification of cellulose nanomaterial for urea biosensor application
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12082/ http://journalarticle.ukm.my/12082/ http://journalarticle.ukm.my/12082/1/09%20Wan%20Elina%20Faradilla.pdf
first_indexed	2023-09-18T20:01:49Z
last_indexed	2023-09-18T20:01:49Z
_version_	1777406899032948736

Surface modification of cellulose nanomaterial for urea biosensor application

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