Saliva sampling of alcoholic participants using three saliva collection methods

The potential of using saliva as a diagnostic fluid is well documented. The aim of this study was to assess the quality and quantity of saliva DNA of alcoholic and non-alcoholic participants using three saliva collection methods; DNA-SalTM (Oasis Diagnostics, USA), Oragene-DNA (DNA Genotek Inc, Onta...

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Main Authors:	Khan, S.S., Jameel, R.A., Razak, F.A., Bakri, M.M.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12035/ http://journalarticle.ukm.my/12035/ http://journalarticle.ukm.my/12035/1/13%20Khan%2C%20S.S.pdf

id	ukm-12035
recordtype	eprints
spelling	ukm-120352018-09-09T23:24:48Z http://journalarticle.ukm.my/12035/ Saliva sampling of alcoholic participants using three saliva collection methods Khan, S.S. Jameel, R.A. Razak, F.A. Bakri, M.M. The potential of using saliva as a diagnostic fluid is well documented. The aim of this study was to assess the quality and quantity of saliva DNA of alcoholic and non-alcoholic participants using three saliva collection methods; DNA-SalTM (Oasis Diagnostics, USA), Oragene-DNA (DNA Genotek Inc, Ontario, Canada) and whole saliva collection method. Saliva DNA of non-alcoholic (n=30) and alcoholic participants (n=10) age between 25 and 35 years was assessed qualitatively and quantitatively using spectrophotometry. Saliva DNA quantity was the highest for all participants when using the DNA-Sal TM saliva collection kit (p<0.05). The use of a mechanical scraper provided only in the DNA-Sal TM kit may have contributed to the highest DNA yield for all participants. The quantity of saliva DNA when assessed using spectrophotometer was found to be significantly lower (p<0.05) for the alcoholic (16±3.57 ng/μL) than non-alcoholic participants (19.92±6.18 ng/μL). To determine the integrity of the DNA samples, PCR amplification of the Alcohol Dehydrogenase gene, ADH1B was carried out and the PCR was found to be successful. For all participants, the DNA quality of the saliva collected using the three saliva collection methods was found to be in the acceptable range considered as pure DNA. The DNA quality and quantity of saliva collected from the three saliva collection methods were considered suitable for research purposes. Penerbit Universiti Kebangsaan Malaysia 2018-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12035/1/13%20Khan%2C%20S.S.pdf Khan, S.S. and Jameel, R.A. and Razak, F.A. and Bakri, M.M. (2018) Saliva sampling of alcoholic participants using three saliva collection methods. Sains Malaysiana, 47 (3). pp. 531-536. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil3_2018/KandunganJilid47Bil3_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The potential of using saliva as a diagnostic fluid is well documented. The aim of this study was to assess the quality and quantity of saliva DNA of alcoholic and non-alcoholic participants using three saliva collection methods; DNA-SalTM (Oasis Diagnostics, USA), Oragene-DNA (DNA Genotek Inc, Ontario, Canada) and whole saliva collection method. Saliva DNA of non-alcoholic (n=30) and alcoholic participants (n=10) age between 25 and 35 years was assessed qualitatively and quantitatively using spectrophotometry. Saliva DNA quantity was the highest for all participants when using the DNA-Sal TM saliva collection kit (p<0.05). The use of a mechanical scraper provided only in the DNA-Sal TM kit may have contributed to the highest DNA yield for all participants. The quantity of saliva DNA when assessed using spectrophotometer was found to be significantly lower (p<0.05) for the alcoholic (16±3.57 ng/μL) than non-alcoholic participants (19.92±6.18 ng/μL). To determine the integrity of the DNA samples, PCR amplification of the Alcohol Dehydrogenase gene, ADH1B was carried out and the PCR was found to be successful. For all participants, the DNA quality of the saliva collected using the three saliva collection methods was found to be in the acceptable range considered as pure DNA. The DNA quality and quantity of saliva collected from the three saliva collection methods were considered suitable for research purposes.
format	Article
author	Khan, S.S. Jameel, R.A. Razak, F.A. Bakri, M.M.
spellingShingle	Khan, S.S. Jameel, R.A. Razak, F.A. Bakri, M.M. Saliva sampling of alcoholic participants using three saliva collection methods
author_facet	Khan, S.S. Jameel, R.A. Razak, F.A. Bakri, M.M.
author_sort	Khan, S.S.
title	Saliva sampling of alcoholic participants using three saliva collection methods
title_short	Saliva sampling of alcoholic participants using three saliva collection methods
title_full	Saliva sampling of alcoholic participants using three saliva collection methods
title_fullStr	Saliva sampling of alcoholic participants using three saliva collection methods
title_full_unstemmed	Saliva sampling of alcoholic participants using three saliva collection methods
title_sort	saliva sampling of alcoholic participants using three saliva collection methods
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12035/ http://journalarticle.ukm.my/12035/ http://journalarticle.ukm.my/12035/1/13%20Khan%2C%20S.S.pdf
first_indexed	2023-09-18T20:01:42Z
last_indexed	2023-09-18T20:01:42Z
_version_	1777406892324159488

Saliva sampling of alcoholic participants using three saliva collection methods

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