Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria

Acetic acid bacteria (AAB) isolated from natural resources and fermented plant beverages were screened to produce 1,3-dihydroxyacetone (DHA) from non-detoxified crude glycerol. Among them, the isolate NKC115 was identified as Gluconobacter frateurii and produced the highest amounts of DHA. Subsequen...

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Main Authors:	Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12029/ http://journalarticle.ukm.my/12029/ http://journalarticle.ukm.my/12029/1/07%20Varavut%20Tanamool.pdf

id	ukm-12029
recordtype	eprints
spelling	ukm-120292018-09-09T23:15:19Z http://journalarticle.ukm.my/12029/ Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol, Acetic acid bacteria (AAB) isolated from natural resources and fermented plant beverages were screened to produce 1,3-dihydroxyacetone (DHA) from non-detoxified crude glycerol. Among them, the isolate NKC115 was identified as Gluconobacter frateurii and produced the highest amounts of DHA. Subsequently, the effects of growth-medium conditions (initial pH, crude glycerol concentration and nitrogen sources) on growth and DHA-production capability were examined. The results showed that the crude glycerol concentration increase to above 100 g/L suppressed growth and DHA production. The highest amount of DHA obtained was 27.50 g/L, from an initial crude glycerol concentration of 100 g/L. Meanwhile, an initial pH of 5.5-7.5 in the YPGc medium did not significantly affect the bacterial growth and DHA production. The optimal nitrogen source was peptone, with DHA production at 34.70 g/L. Furthermore, overexpression of the nhaK2 gene encoding for the Na+(K+)/H+ antiporter from Acetobactor tropicalis SKU1100 in G. frateurii NKC115 improved growth and increased the accumulation of DHA (37.25 g/L) from an initial crude glycerol concentration of 20%. These results indicated that the expression of this antiporter might maintain an optimal intracellular pH and concentration of Na+ or K+, leading to the cells’ ability to tolerate high concentrations of crude glycerol. Penerbit Universiti Kebangsaan Malaysia 2018-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12029/1/07%20Varavut%20Tanamool.pdf Varavut Tanamool, and Piyorot Hongsachart, and Wichai Soemphol, (2018) Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria. Sains Malaysiana, 47 (3). pp. 481-488. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil3_2018/KandunganJilid47Bil3_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Acetic acid bacteria (AAB) isolated from natural resources and fermented plant beverages were screened to produce 1,3-dihydroxyacetone (DHA) from non-detoxified crude glycerol. Among them, the isolate NKC115 was identified as Gluconobacter frateurii and produced the highest amounts of DHA. Subsequently, the effects of growth-medium conditions (initial pH, crude glycerol concentration and nitrogen sources) on growth and DHA-production capability were examined. The results showed that the crude glycerol concentration increase to above 100 g/L suppressed growth and DHA production. The highest amount of DHA obtained was 27.50 g/L, from an initial crude glycerol concentration of 100 g/L. Meanwhile, an initial pH of 5.5-7.5 in the YPGc medium did not significantly affect the bacterial growth and DHA production. The optimal nitrogen source was peptone, with DHA production at 34.70 g/L. Furthermore, overexpression of the nhaK2 gene encoding for the Na+(K+)/H+ antiporter from Acetobactor tropicalis SKU1100 in G. frateurii NKC115 improved growth and increased the accumulation of DHA (37.25 g/L) from an initial crude glycerol concentration of 20%. These results indicated that the expression of this antiporter might maintain an optimal intracellular pH and concentration of Na+ or K+, leading to the cells’ ability to tolerate high concentrations of crude glycerol.
format	Article
author	Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol,
spellingShingle	Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol, Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria
author_facet	Varavut Tanamool, Piyorot Hongsachart, Wichai Soemphol,
author_sort	Varavut Tanamool,
title	Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria
title_short	Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria
title_full	Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria
title_fullStr	Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria
title_full_unstemmed	Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria
title_sort	bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12029/ http://journalarticle.ukm.my/12029/ http://journalarticle.ukm.my/12029/1/07%20Varavut%20Tanamool.pdf
first_indexed	2023-09-18T20:01:42Z
last_indexed	2023-09-18T20:01:42Z
_version_	1777406891444404224

Bioconversion of biodiesel-derived crude glycerol to 1,3-dihydroxyacetone by a potential acetic acid bacteria

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