Characterization and screening of lipolytic bacteria from Thai fermented fish

Nine bacterial strains were isolated from Thai fermented fish by the standard dilution technique using JCM no. 377 medium. The rod shaped isolate FN2-3 was identified as Virgibacillus dokdonensis while isolates FN2-3 and FN6-6 as V. halodenitrificans, FN1-13 as Corynebacterium variabile, FN1-10 as O...

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Main Authors:	Rungsima Daroonpunt, Naoto Tanaka, Masataka Uchino, Somboon Tanasupawat
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/11984/ http://journalarticle.ukm.my/11984/ http://journalarticle.ukm.my/11984/1/11%20Rungsima%20Daroonpunt.pdf

id	ukm-11984
recordtype	eprints
spelling	ukm-119842018-08-14T00:29:13Z http://journalarticle.ukm.my/11984/ Characterization and screening of lipolytic bacteria from Thai fermented fish Rungsima Daroonpunt, Naoto Tanaka, Masataka Uchino, Somboon Tanasupawat, Nine bacterial strains were isolated from Thai fermented fish by the standard dilution technique using JCM no. 377 medium. The rod shaped isolate FN2-3 was identified as Virgibacillus dokdonensis while isolates FN2-3 and FN6-6 as V. halodenitrificans, FN1-13 as Corynebacterium variabile, FN1-10 as Oceanobacillus iheyensis and FN3-7 was Bacillus amyloliquefaciens subsp. plantarum. The coccal isolates, FN6-1 and FN6-7 were Staphylococcus saprophyticus subsp. bovis and FN6-8 was S. saprophyticus subsp. saprophyticus, based on their phenotypic characteristics and 16S rRNA gene sequence analyses at a 99.63-100% sequence similarity. Their lipase activity in complex medium (CM), CM medium with 1% (v/v) Tween 20 or CM medium with 1% (v/v) Tween 80 ranged from 1.12 ± 0.03-3.77 ± 0.04 unit/mL, with the highest lipase activity found with V. dokdonensis FN1-8 cultivated with CM with 1% (v/v) Tween 80. Penerbit Universiti Kebangsaan Malaysia 2018-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11984/1/11%20Rungsima%20Daroonpunt.pdf Rungsima Daroonpunt, and Naoto Tanaka, and Masataka Uchino, and Somboon Tanasupawat, (2018) Characterization and screening of lipolytic bacteria from Thai fermented fish. Sains Malaysiana, 47 (1). pp. 91-97. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num1_2018/contentsVol47num1_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Nine bacterial strains were isolated from Thai fermented fish by the standard dilution technique using JCM no. 377 medium. The rod shaped isolate FN2-3 was identified as Virgibacillus dokdonensis while isolates FN2-3 and FN6-6 as V. halodenitrificans, FN1-13 as Corynebacterium variabile, FN1-10 as Oceanobacillus iheyensis and FN3-7 was Bacillus amyloliquefaciens subsp. plantarum. The coccal isolates, FN6-1 and FN6-7 were Staphylococcus saprophyticus subsp. bovis and FN6-8 was S. saprophyticus subsp. saprophyticus, based on their phenotypic characteristics and 16S rRNA gene sequence analyses at a 99.63-100% sequence similarity. Their lipase activity in complex medium (CM), CM medium with 1% (v/v) Tween 20 or CM medium with 1% (v/v) Tween 80 ranged from 1.12 ± 0.03-3.77 ± 0.04 unit/mL, with the highest lipase activity found with V. dokdonensis FN1-8 cultivated with CM with 1% (v/v) Tween 80.
format	Article
author	Rungsima Daroonpunt, Naoto Tanaka, Masataka Uchino, Somboon Tanasupawat,
spellingShingle	Rungsima Daroonpunt, Naoto Tanaka, Masataka Uchino, Somboon Tanasupawat, Characterization and screening of lipolytic bacteria from Thai fermented fish
author_facet	Rungsima Daroonpunt, Naoto Tanaka, Masataka Uchino, Somboon Tanasupawat,
author_sort	Rungsima Daroonpunt,
title	Characterization and screening of lipolytic bacteria from Thai fermented fish
title_short	Characterization and screening of lipolytic bacteria from Thai fermented fish
title_full	Characterization and screening of lipolytic bacteria from Thai fermented fish
title_fullStr	Characterization and screening of lipolytic bacteria from Thai fermented fish
title_full_unstemmed	Characterization and screening of lipolytic bacteria from Thai fermented fish
title_sort	characterization and screening of lipolytic bacteria from thai fermented fish
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/11984/ http://journalarticle.ukm.my/11984/ http://journalarticle.ukm.my/11984/1/11%20Rungsima%20Daroonpunt.pdf
first_indexed	2023-09-18T20:01:35Z
last_indexed	2023-09-18T20:01:35Z
_version_	1777406884837326848

Characterization and screening of lipolytic bacteria from Thai fermented fish

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