A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen

A novel silver nanoparticles (Ag NPs)-based optical sensing probe has been developed for the detection of Japanese Encephalitis virus (JEV). Ag NPs were initially deposited onto amine functionalized glass slides. Subsequently, JEV antibodies were self-assembled onto surfaces of Ag NPs to form optica...

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Main Authors:	Lim, Lih Shan, Chin, Suk Fun, Pang, Suh Cem, Magdline Sia Henry Sum, David Perera
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11713/ http://journalarticle.ukm.my/11713/ http://journalarticle.ukm.my/11713/1/21%20Lih%20Shan%20Lim.pdf

id	ukm-11713
recordtype	eprints
spelling	ukm-117132018-05-30T00:52:21Z http://journalarticle.ukm.my/11713/ A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen Lim, Lih Shan Chin, Suk Fun Pang, Suh Cem Magdline Sia Henry Sum, David Perera, A novel silver nanoparticles (Ag NPs)-based optical sensing probe has been developed for the detection of Japanese Encephalitis virus (JEV). Ag NPs were initially deposited onto amine functionalized glass slides. Subsequently, JEV antibodies were self-assembled onto surfaces of Ag NPs to form optical sensing probes. The detection of JEV antigen was observed via changes in light absorbance by Ag NPs upon occurrence of JEV antigen-antibody bindings. A highly sensitive and rapid optical sensing probe for JEV antigen with a detection limit of 12.8 ng/mL (for S/N ratio = 3) and an analysis assay time of 1 h had been demonstrated. Penerbit Universiti Kebangsaan Malaysia 2017-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11713/1/21%20Lih%20Shan%20Lim.pdf Lim, Lih Shan and Chin, Suk Fun and Pang, Suh Cem and Magdline Sia Henry Sum, and David Perera, (2017) A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen. Sains Malaysiana, 46 (12). pp. 2447-2454. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num12_2017/contentsVol46num12_2017.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A novel silver nanoparticles (Ag NPs)-based optical sensing probe has been developed for the detection of Japanese Encephalitis virus (JEV). Ag NPs were initially deposited onto amine functionalized glass slides. Subsequently, JEV antibodies were self-assembled onto surfaces of Ag NPs to form optical sensing probes. The detection of JEV antigen was observed via changes in light absorbance by Ag NPs upon occurrence of JEV antigen-antibody bindings. A highly sensitive and rapid optical sensing probe for JEV antigen with a detection limit of 12.8 ng/mL (for S/N ratio = 3) and an analysis assay time of 1 h had been demonstrated.
format	Article
author	Lim, Lih Shan Chin, Suk Fun Pang, Suh Cem Magdline Sia Henry Sum, David Perera,
spellingShingle	Lim, Lih Shan Chin, Suk Fun Pang, Suh Cem Magdline Sia Henry Sum, David Perera, A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen
author_facet	Lim, Lih Shan Chin, Suk Fun Pang, Suh Cem Magdline Sia Henry Sum, David Perera,
author_sort	Lim, Lih Shan
title	A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen
title_short	A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen
title_full	A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen
title_fullStr	A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen
title_full_unstemmed	A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen
title_sort	novel silver nanoparticles-based sensing probe for the detection of japanese encephalitis virus antigen
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11713/ http://journalarticle.ukm.my/11713/ http://journalarticle.ukm.my/11713/1/21%20Lih%20Shan%20Lim.pdf
first_indexed	2023-09-18T20:00:58Z
last_indexed	2023-09-18T20:00:58Z
_version_	1777406845633167360

A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen

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