Characteristics of human spermatozoa harvested in culture media with and without serum proteins

This study was aimed to determine the efficiency of synthetic protein-free media in spermatozoa washing, preparation and retention of the activity of washed spermatozoa over short periods in vitro. Normozoospermic semen samples (n = 71) were equally apportioned and washed using synthetic protein-fre...

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Main Authors:	Ghofraan A. Ata’Allah, Noor Azmi Mat Adenan, Nuguelis Razali, Siti Khadijah Idris, Jaffar Ali, Kannappan Palaniappan, Rosliza Saad, Krishnan Kanniah
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/11508/ http://journalarticle.ukm.my/11508/ http://journalarticle.ukm.my/11508/1/18649-69650-1-PB.pdf

id	ukm-11508
recordtype	eprints
spelling	ukm-115082018-04-02T02:49:43Z http://journalarticle.ukm.my/11508/ Characteristics of human spermatozoa harvested in culture media with and without serum proteins Ghofraan A. Ata’Allah, Noor Azmi Mat Adenan, Nuguelis Razali, Siti Khadijah Idris, Jaffar Ali, Kannappan Palaniappan, Rosliza Saad, Krishnan Kanniah, This study was aimed to determine the efficiency of synthetic protein-free media in spermatozoa washing, preparation and retention of the activity of washed spermatozoa over short periods in vitro. Normozoospermic semen samples (n = 71) were equally apportioned and washed using synthetic protein-free medium (PFM), minimum essential medium + HSA (MEM) or commercial protein-containing medium (CPC). Washed spermatozoa were cultured in vitro using PFM, MEM or CPC media and held for 24 hrs at 4°C, 15°C, 22°C or 37°C. Spermatozoa activity was evaluated at 0 hr, 4 to 7 hrs and 24 hrs post-wash. The effects of PFM on spermatozoa motility, vitality, membrane integrity and DNA fragmentation level were not significantly different from that of MEM and CPC media at 0 hr, 4 to 7 hrs and 24 hrs post-wash in vitro. Synthetic PFM, MEM and CPC retained spermatozoa activity highest when specimen were held at 22°C and it was significantly higher (p < 0.05) than that at 37°C after 24 hrs incubation in vitro. However, no significant changes (p > 0.05) were noted in spermatozoa DNA fragmentation (SDF) levels when specimen were held at 22°C or 37°C at 4 to 7 hrs and also after 24 hrs post-wash in vitro in all media. The use of synthetic PFM as an alternative to the commercial protein-containing media in human spermatozoa washing and preparation procedure for an efficient and safer (Assisted Reproduction Technology) ART outcome. Spermatozoa activity can be successfully retained at room temperature post-wash over short periods; spermatozoa may lose viability rapidly if held for long hours at 37°C in all media. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11508/1/18649-69650-1-PB.pdf Ghofraan A. Ata’Allah, and Noor Azmi Mat Adenan, and Nuguelis Razali, and Siti Khadijah Idris, and Jaffar Ali, and Kannappan Palaniappan, and Rosliza Saad, and Krishnan Kanniah, (2018) Characteristics of human spermatozoa harvested in culture media with and without serum proteins. Jurnal Sains Kesihatan Malaysia, 16 (1). pp. 125-134. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/684
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was aimed to determine the efficiency of synthetic protein-free media in spermatozoa washing, preparation and retention of the activity of washed spermatozoa over short periods in vitro. Normozoospermic semen samples (n = 71) were equally apportioned and washed using synthetic protein-free medium (PFM), minimum essential medium + HSA (MEM) or commercial protein-containing medium (CPC). Washed spermatozoa were cultured in vitro using PFM, MEM or CPC media and held for 24 hrs at 4°C, 15°C, 22°C or 37°C. Spermatozoa activity was evaluated at 0 hr, 4 to 7 hrs and 24 hrs post-wash. The effects of PFM on spermatozoa motility, vitality, membrane integrity and DNA fragmentation level were not significantly different from that of MEM and CPC media at 0 hr, 4 to 7 hrs and 24 hrs post-wash in vitro. Synthetic PFM, MEM and CPC retained spermatozoa activity highest when specimen were held at 22°C and it was significantly higher (p < 0.05) than that at 37°C after 24 hrs incubation in vitro. However, no significant changes (p > 0.05) were noted in spermatozoa DNA fragmentation (SDF) levels when specimen were held at 22°C or 37°C at 4 to 7 hrs and also after 24 hrs post-wash in vitro in all media. The use of synthetic PFM as an alternative to the commercial protein-containing media in human spermatozoa washing and preparation procedure for an efficient and safer (Assisted Reproduction Technology) ART outcome. Spermatozoa activity can be successfully retained at room temperature post-wash over short periods; spermatozoa may lose viability rapidly if held for long hours at 37°C in all media.
format	Article
author	Ghofraan A. Ata’Allah, Noor Azmi Mat Adenan, Nuguelis Razali, Siti Khadijah Idris, Jaffar Ali, Kannappan Palaniappan, Rosliza Saad, Krishnan Kanniah,
spellingShingle	Ghofraan A. Ata’Allah, Noor Azmi Mat Adenan, Nuguelis Razali, Siti Khadijah Idris, Jaffar Ali, Kannappan Palaniappan, Rosliza Saad, Krishnan Kanniah, Characteristics of human spermatozoa harvested in culture media with and without serum proteins
author_facet	Ghofraan A. Ata’Allah, Noor Azmi Mat Adenan, Nuguelis Razali, Siti Khadijah Idris, Jaffar Ali, Kannappan Palaniappan, Rosliza Saad, Krishnan Kanniah,
author_sort	Ghofraan A. Ata’Allah,
title	Characteristics of human spermatozoa harvested in culture media with and without serum proteins
title_short	Characteristics of human spermatozoa harvested in culture media with and without serum proteins
title_full	Characteristics of human spermatozoa harvested in culture media with and without serum proteins
title_fullStr	Characteristics of human spermatozoa harvested in culture media with and without serum proteins
title_full_unstemmed	Characteristics of human spermatozoa harvested in culture media with and without serum proteins
title_sort	characteristics of human spermatozoa harvested in culture media with and without serum proteins
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/11508/ http://journalarticle.ukm.my/11508/ http://journalarticle.ukm.my/11508/1/18649-69650-1-PB.pdf
first_indexed	2023-09-18T19:01:02Z
last_indexed	2023-09-18T19:01:02Z
_version_	1777406817434861568

Characteristics of human spermatozoa harvested in culture media with and without serum proteins

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