Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16

Polyhydroxyalkanoates (PHAs) can be used to replace petrochemical plastics in many applications. However, their production has limitation due to production cost. This research was conducted using alternative carbon source from waste to synthesize PHAs by Cupriavidus necator H16. In this study, PHAs...

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Main Authors:	Chatsuda Kongpeng, Jutarut Iewkittayakorn, Wilaiwan Chotigeat
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11372/ http://journalarticle.ukm.my/11372/ http://journalarticle.ukm.my/11372/1/15%20Chatsuda.pdf

id	ukm-11372
recordtype	eprints
spelling	ukm-113722018-02-15T05:14:26Z http://journalarticle.ukm.my/11372/ Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16 Chatsuda Kongpeng, Jutarut Iewkittayakorn, Wilaiwan Chotigeat, Polyhydroxyalkanoates (PHAs) can be used to replace petrochemical plastics in many applications. However, their production has limitation due to production cost. This research was conducted using alternative carbon source from waste to synthesize PHAs by Cupriavidus necator H16. In this study, PHAs were produced from used cooking oil (UCO) and compared with production PHAs from palm oil. Two UCO storage times (4 and 10 weeks) and three UCO concentrations (10, 20 and 30 g/L) were used to determine the most optimum condition for PHA synthesis. The best optimum condition for PHA synthesis was cultivated in the medium containing 30 g/L of UCO at 4 weeks storage. The cell dry weight (CDW) and PHA content were 5.26±0.61 g/L and 27.36±2.04 wt. %, respectively. These results were similar to cell cultivation using 20 g/L palm oil and 1% of fructose (5.93±0.33 g/L of CDW and 26.96±6.14 wt. % of PHA contents). In addition, PHA content from the culture with 10 g/L of UCO stored for 10 weeks was higher than PHA content from the culture with 20 and 30 g/L of UCO. Thus, it can be concluded that UCO could be used in PHA production. Penerbit Universiti Kebangsaan Malaysia 2017-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11372/1/15%20Chatsuda.pdf Chatsuda Kongpeng, and Jutarut Iewkittayakorn, and Wilaiwan Chotigeat, (2017) Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16. Sains Malaysiana, 46 (9). pp. 1465-1469. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Polyhydroxyalkanoates (PHAs) can be used to replace petrochemical plastics in many applications. However, their production has limitation due to production cost. This research was conducted using alternative carbon source from waste to synthesize PHAs by Cupriavidus necator H16. In this study, PHAs were produced from used cooking oil (UCO) and compared with production PHAs from palm oil. Two UCO storage times (4 and 10 weeks) and three UCO concentrations (10, 20 and 30 g/L) were used to determine the most optimum condition for PHA synthesis. The best optimum condition for PHA synthesis was cultivated in the medium containing 30 g/L of UCO at 4 weeks storage. The cell dry weight (CDW) and PHA content were 5.26±0.61 g/L and 27.36±2.04 wt. %, respectively. These results were similar to cell cultivation using 20 g/L palm oil and 1% of fructose (5.93±0.33 g/L of CDW and 26.96±6.14 wt. % of PHA contents). In addition, PHA content from the culture with 10 g/L of UCO stored for 10 weeks was higher than PHA content from the culture with 20 and 30 g/L of UCO. Thus, it can be concluded that UCO could be used in PHA production.
format	Article
author	Chatsuda Kongpeng, Jutarut Iewkittayakorn, Wilaiwan Chotigeat,
spellingShingle	Chatsuda Kongpeng, Jutarut Iewkittayakorn, Wilaiwan Chotigeat, Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16
author_facet	Chatsuda Kongpeng, Jutarut Iewkittayakorn, Wilaiwan Chotigeat,
author_sort	Chatsuda Kongpeng,
title	Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16
title_short	Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16
title_full	Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16
title_fullStr	Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16
title_full_unstemmed	Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16
title_sort	effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (phas) production by cupriavidus necator h16
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11372/ http://journalarticle.ukm.my/11372/ http://journalarticle.ukm.my/11372/1/15%20Chatsuda.pdf
first_indexed	2023-09-18T20:00:07Z
last_indexed	2023-09-18T20:00:07Z
_version_	1777406791701757952

Effect of storage time and concentration of used cooking oil on polyhydroxyalkanoates (PHAs) production by Cupriavidus necator H16

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