$Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis$

Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis

This study was carried out to identify the active compounds of Emilia sonchifolia on induction of ovarian maturation in Fenneropenaeus merguiensis. The crude extracts from dichloromethane and acetone were investigated in vitro. The crude extract from acetone induced up-regulation of shrimp ovarian p...

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Main Authors:	Lamai Maikaeo, Wilawan Mahabusarakam, Wilaiwan Chotigeat
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11371/ http://journalarticle.ukm.my/11371/ http://journalarticle.ukm.my/11371/1/14%20Lamai%20Mikaeo.pdf

id	ukm-11371
recordtype	eprints
spelling	ukm-113712018-02-15T05:12:13Z http://journalarticle.ukm.my/11371/ Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis Lamai Maikaeo, Wilawan Mahabusarakam, Wilaiwan Chotigeat, This study was carried out to identify the active compounds of Emilia sonchifolia on induction of ovarian maturation in Fenneropenaeus merguiensis. The crude extracts from dichloromethane and acetone were investigated in vitro. The crude extract from acetone induced up-regulation of shrimp ovarian peritrophin (SOP) and translationally controlled tumor protein (TCTP) genes, which were highly expressed during early phase of ovarian development higher than dichloromethane extract. Furthermore, fraction 14 (F14) from acetone extract up-regulated both of the SOP and the TCTP genes to the greatest extent. Leading to in vivo study, the effect of ribosomal protein L10a (RPL10a), which acts as shrimp ovarian stimulator plus with F14 on shrimp ovarian maturation was investigated by injection. The best result was observed in the group that received RPL10a plus with F14 at 0.8 μg/g body weight of shrimp. The RPL10a plus F14 enhanced the shrimp ovaries from undeveloped stage to 57% of early developing stage within 15 days. Meanwhile, the control group remained 100% of the undeveloped stage. Hence, F14 seems to play a positive role in the induction of shrimp ovarian maturation. The component of F14 was identified using mass spectroscopy and presented as 1,2-benzenedicarboxylic acid; 2,4-di-tert-butyl phenol; 2,4,5-trimethoybenzylidene;palmitic acid; 1-heneicosyl formate; 1-heptadecanol; ethyl-4-ethoxybenzoate and stearic acid. Penerbit Universiti Kebangsaan Malaysia 2017-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11371/1/14%20Lamai%20Mikaeo.pdf Lamai Maikaeo, and Wilawan Mahabusarakam, and Wilaiwan Chotigeat, (2017) Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis. Sains Malaysiana, 46 (9). pp. 1457-1464. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num9_2017/contentsVol46num9_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was carried out to identify the active compounds of Emilia sonchifolia on induction of ovarian maturation in Fenneropenaeus merguiensis. The crude extracts from dichloromethane and acetone were investigated in vitro. The crude extract from acetone induced up-regulation of shrimp ovarian peritrophin (SOP) and translationally controlled tumor protein (TCTP) genes, which were highly expressed during early phase of ovarian development higher than dichloromethane extract. Furthermore, fraction 14 (F14) from acetone extract up-regulated both of the SOP and the TCTP genes to the greatest extent. Leading to in vivo study, the effect of ribosomal protein L10a (RPL10a), which acts as shrimp ovarian stimulator plus with F14 on shrimp ovarian maturation was investigated by injection. The best result was observed in the group that received RPL10a plus with F14 at 0.8 μg/g body weight of shrimp. The RPL10a plus F14 enhanced the shrimp ovaries from undeveloped stage to 57% of early developing stage within 15 days. Meanwhile, the control group remained 100% of the undeveloped stage. Hence, F14 seems to play a positive role in the induction of shrimp ovarian maturation. The component of F14 was identified using mass spectroscopy and presented as 1,2-benzenedicarboxylic acid; 2,4-di-tert-butyl phenol; 2,4,5-trimethoybenzylidene;palmitic acid; 1-heneicosyl formate; 1-heptadecanol; ethyl-4-ethoxybenzoate and stearic acid.
format	Article
author	Lamai Maikaeo, Wilawan Mahabusarakam, Wilaiwan Chotigeat,
spellingShingle	Lamai Maikaeo, Wilawan Mahabusarakam, Wilaiwan Chotigeat, Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis
author_facet	Lamai Maikaeo, Wilawan Mahabusarakam, Wilaiwan Chotigeat,
author_sort	Lamai Maikaeo,
title	Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis
title_short	Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis
title_full	Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis
title_fullStr	Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis
title_full_unstemmed	Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis
title_sort	bioassay-guided fractionation of emilia sonchifolia extract on the induction of ovarian maturation in fenneropenaeus merguiensis
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11371/ http://journalarticle.ukm.my/11371/ http://journalarticle.ukm.my/11371/1/14%20Lamai%20Mikaeo.pdf
first_indexed	2023-09-18T20:00:06Z
last_indexed	2023-09-18T20:00:06Z
_version_	1777406791554957312

Bioassay-guided fractionation of Emilia sonchifolia extract on the induction of ovarian maturation in Fenneropenaeus merguiensis

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