Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines

According to the World Cancer Research Fund International (WCRFI), breast cancer is the most common type of cancer in women worldwide with recorded 1.7 million new cases in 2012. The main line of treatments is still limited to chemotherapy, surgery and radiotherapy which could lead to a wide range o...

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Main Authors:	Siti P.M. Bohari, Hamidreza Aboulkheyr, E.S., Nur S. Johan, Nursyuhada F. Zainudin
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11034/ http://journalarticle.ukm.my/11034/ http://journalarticle.ukm.my/11034/1/09%20Siti%20P.M.%20Bohari.pdf

id	ukm-11034
recordtype	eprints
spelling	ukm-110342017-11-27T12:10:29Z http://journalarticle.ukm.my/11034/ Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines Siti P.M. Bohari, Hamidreza Aboulkheyr, E.S., Nur S. Johan, Nursyuhada F. Zainudin, According to the World Cancer Research Fund International (WCRFI), breast cancer is the most common type of cancer in women worldwide with recorded 1.7 million new cases in 2012. The main line of treatments is still limited to chemotherapy, surgery and radiotherapy which could lead to a wide range of dangerous side effects. This study was conducted to evaluate the effect of low intensity ultrasound (LIUS) on cell proliferation, percentage of living and dead cells and the induction of apoptosis on the MCF-7 cell line with CHO cells as the control for non-cancerous group. In order to achieve the objective of this study, several methods of cell-bioguided assays were used including the MTT assay for cell proliferation, Live/Dead assay for the determination of both live and dead cells and gene expression study for the detection of apoptosis in the cells. The cytotoxicity and Live/Dead assays data provided preliminary data that the LIUS has potential to induce apoptosis in a wide population of breast cancer cells. Furthermore, the LIUS treatment induced the expression of p53-mRNA at a detectable level via qPCR analysis, indicating the activation of apoptosis. In short, our study suggested LIUS dosage used in this study could potentially show positive effects in the induction of apoptosis selectively on the MCF-7 with less harm to the control CHO cells. Penerbit Universiti Kebangsaan Malaysia 2017-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11034/1/09%20Siti%20P.M.%20Bohari.pdf Siti P.M. Bohari, and Hamidreza Aboulkheyr, and E.S., and Nur S. Johan, and Nursyuhada F. Zainudin, (2017) Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines. Sains Malaysiana, 46 (4). pp. 575-581. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num4_2017/contentsVol46num4_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	According to the World Cancer Research Fund International (WCRFI), breast cancer is the most common type of cancer in women worldwide with recorded 1.7 million new cases in 2012. The main line of treatments is still limited to chemotherapy, surgery and radiotherapy which could lead to a wide range of dangerous side effects. This study was conducted to evaluate the effect of low intensity ultrasound (LIUS) on cell proliferation, percentage of living and dead cells and the induction of apoptosis on the MCF-7 cell line with CHO cells as the control for non-cancerous group. In order to achieve the objective of this study, several methods of cell-bioguided assays were used including the MTT assay for cell proliferation, Live/Dead assay for the determination of both live and dead cells and gene expression study for the detection of apoptosis in the cells. The cytotoxicity and Live/Dead assays data provided preliminary data that the LIUS has potential to induce apoptosis in a wide population of breast cancer cells. Furthermore, the LIUS treatment induced the expression of p53-mRNA at a detectable level via qPCR analysis, indicating the activation of apoptosis. In short, our study suggested LIUS dosage used in this study could potentially show positive effects in the induction of apoptosis selectively on the MCF-7 with less harm to the control CHO cells.
format	Article
author	Siti P.M. Bohari, Hamidreza Aboulkheyr, E.S., Nur S. Johan, Nursyuhada F. Zainudin,
spellingShingle	Siti P.M. Bohari, Hamidreza Aboulkheyr, E.S., Nur S. Johan, Nursyuhada F. Zainudin, Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines
author_facet	Siti P.M. Bohari, Hamidreza Aboulkheyr, E.S., Nur S. Johan, Nursyuhada F. Zainudin,
author_sort	Siti P.M. Bohari,
title	Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines
title_short	Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines
title_full	Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines
title_fullStr	Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines
title_full_unstemmed	Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines
title_sort	low intensity ultrasound induced apoptosis in mcf-7 breast cancer cell lines
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11034/ http://journalarticle.ukm.my/11034/ http://journalarticle.ukm.my/11034/1/09%20Siti%20P.M.%20Bohari.pdf
first_indexed	2023-09-18T19:59:09Z
last_indexed	2023-09-18T19:59:09Z
_version_	1777406731070996480

Low intensity ultrasound induced apoptosis in MCF-7 breast cancer cell lines

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