Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology

Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology

Response surface analysis was conducted to optimize the concentrations of Tapis crude oil and duration of incubation in order to achieve optimal microbial growth and crude oil biodegradation. Central Composite Rotatable Design (CCRD) was employed, where the design contained 13 experimental runs with...

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Main Authors: Ainon Hamzah, Vidyah Manikan, Nur Ain Fathiha Abd Aziz
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia IP 2017
Online Access:http://journalarticle.ukm.my/10591/
http://journalarticle.ukm.my/10591/
http://journalarticle.ukm.my/10591/1/06%20Ainon%20Hamzah.pdf
id ukm-10591
recordtype eprints
spelling ukm-105912017-08-20T08:19:17Z http://journalarticle.ukm.my/10591/ Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology Ainon Hamzah, Vidyah Manikan, Nur Ain Fathiha Abd Aziz, Response surface analysis was conducted to optimize the concentrations of Tapis crude oil and duration of incubation in order to achieve optimal microbial growth and crude oil biodegradation. Central Composite Rotatable Design (CCRD) was employed, where the design contained 13 experimental runs with different combinations of incubation time and crude oil concentration. The cultures containing mineral salt medium (MSM) with varying crude oil concentrations were incubated at 30°C, pH6.5 with 150 rpm agitation for 120-336 h. The inoculum contained a consortium of previously identified as oil degrading bacteria and fungi, namely Pseudomonas aeruginosa UKMP-14T, Acinetobacter baumannii UKMP-12T and Trichoderma sp. UKMP-1M and UKMP-2M. The analysis showed the duration of incubation plays a significant role (p<0.05) in affecting the bacterial growth and percentage of total petroleum hydrocarbon (TPH) biodegradation, meanwhile concentrations of Tapis crude oil has insignificant effect on the responses. Interaction of the two variables was found to be significant in affecting all the three responses, namely bacterial population, fungal biomass and percentage of TPH biodegradation. It was predicted through the CCRD that the percentage of biodegradation can be optimized to reach 86% on the 270th h when 5% (v/v) crude oil was used. This predicted value was verified to be achievable and reproducible through validation experiments. Penerbit Universiti Kebangsaan Malaysia IP 2017-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10591/1/06%20Ainon%20Hamzah.pdf Ainon Hamzah, and Vidyah Manikan, and Nur Ain Fathiha Abd Aziz, (2017) Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology. Sains Malaysiana, 46 (1). pp. 43-50. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num1_2017/contentsVol46num1_2017.html
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Response surface analysis was conducted to optimize the concentrations of Tapis crude oil and duration of incubation in order to achieve optimal microbial growth and crude oil biodegradation. Central Composite Rotatable Design (CCRD) was employed, where the design contained 13 experimental runs with different combinations of incubation time and crude oil concentration. The cultures containing mineral salt medium (MSM) with varying crude oil concentrations were incubated at 30°C, pH6.5 with 150 rpm agitation for 120-336 h. The inoculum contained a consortium of previously identified as oil degrading bacteria and fungi, namely Pseudomonas aeruginosa UKMP-14T, Acinetobacter baumannii UKMP-12T and Trichoderma sp. UKMP-1M and UKMP-2M. The analysis showed the duration of incubation plays a significant role (p<0.05) in affecting the bacterial growth and percentage of total petroleum hydrocarbon (TPH) biodegradation, meanwhile concentrations of Tapis crude oil has insignificant effect on the responses. Interaction of the two variables was found to be significant in affecting all the three responses, namely bacterial population, fungal biomass and percentage of TPH biodegradation. It was predicted through the CCRD that the percentage of biodegradation can be optimized to reach 86% on the 270th h when 5% (v/v) crude oil was used. This predicted value was verified to be achievable and reproducible through validation experiments.
format Article
author Ainon Hamzah,
Vidyah Manikan,
Nur Ain Fathiha Abd Aziz,
spellingShingle Ainon Hamzah,
Vidyah Manikan,
Nur Ain Fathiha Abd Aziz,
Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology
author_facet Ainon Hamzah,
Vidyah Manikan,
Nur Ain Fathiha Abd Aziz,
author_sort Ainon Hamzah,
title Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology
title_short Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology
title_full Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology
title_fullStr Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology
title_full_unstemmed Biodegradation of Tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by Response Surface Methodology
title_sort biodegradation of tapis crude oil using consortium of bacteria and fungi: optimization of crude oil concentration and duration of incubation by response surface methodology
publisher Penerbit Universiti Kebangsaan Malaysia IP
publishDate 2017
url http://journalarticle.ukm.my/10591/
http://journalarticle.ukm.my/10591/
http://journalarticle.ukm.my/10591/1/06%20Ainon%20Hamzah.pdf
first_indexed 2023-09-18T19:57:54Z
last_indexed 2023-09-18T19:57:54Z
_version_ 1777406652419407872

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