Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Advances in wheat biotechnology and in vitro somatic embryogenesis (SE) provide new openings to faster application of these techniques in functional genomic studies, genetic engineering and plant breeding. This study reports in vitro SE using plumule and radicle explants of two wheat cultivars Cakma...

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Main Authors:	Kahriz, Parisa Pourali, Kahriz, Mahsa Pourali
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/10590/ http://journalarticle.ukm.my/10590/ http://journalarticle.ukm.my/10590/1/05%20Parisa%20Pourali%20Kahriz.pdf

id	ukm-10590
recordtype	eprints
spelling	ukm-105902017-08-22T01:13:12Z http://journalarticle.ukm.my/10590/ Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds Kahriz, Parisa Pourali Kahriz, Mahsa Pourali Advances in wheat biotechnology and in vitro somatic embryogenesis (SE) provide new openings to faster application of these techniques in functional genomic studies, genetic engineering and plant breeding. This study reports in vitro SE using plumule and radicle explants of two wheat cultivars Cakmak and Kunduru on MS induction medium amended with varying concentrations of 2,4-D. Both plumule and radicle explants were regenerative and induced variable number of somatic embryos per explant. Explants and treatment methods affected in vitro SE irrespective of the genotypes used in the study. Although 100% SE was noted on both explants of two cultivars, plumule explants of both genotypes induced heavy and larger friable calli followed by somatic embryogenic calli in terms of weight. Plumule explants also induced large numbers of shoot buds and somatic embryo-induced maturing shoots per explant. The in vitro raised plantlets were successfully rooted followed by hardening and acclimatisation in pots containing peat moss in mist house. After 7 days from weaning, these plants were transferred to a shade house to grow and set flowers. Water spray containing 0.50 μg of NPK (1:1:1) was given at intervals of 4 d to enhance success rate of acclimatised plants. Success rates of 96 and 80% were noted on plumule and radicle-induced plants, respectively. It is further concluded that use of this novel methodology would favorably facilitate its use in genetic transformation and functional genomic studies. Penerbit Universiti Kebangsaan Malaysia 2017-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10590/1/05%20Parisa%20Pourali%20Kahriz.pdf Kahriz, Parisa Pourali and Kahriz, Mahsa Pourali (2017) Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds. Sains Malaysiana, 46 (1). pp. 35-41. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num1_2017/contentsVol46num1_2017.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Advances in wheat biotechnology and in vitro somatic embryogenesis (SE) provide new openings to faster application of these techniques in functional genomic studies, genetic engineering and plant breeding. This study reports in vitro SE using plumule and radicle explants of two wheat cultivars Cakmak and Kunduru on MS induction medium amended with varying concentrations of 2,4-D. Both plumule and radicle explants were regenerative and induced variable number of somatic embryos per explant. Explants and treatment methods affected in vitro SE irrespective of the genotypes used in the study. Although 100% SE was noted on both explants of two cultivars, plumule explants of both genotypes induced heavy and larger friable calli followed by somatic embryogenic calli in terms of weight. Plumule explants also induced large numbers of shoot buds and somatic embryo-induced maturing shoots per explant. The in vitro raised plantlets were successfully rooted followed by hardening and acclimatisation in pots containing peat moss in mist house. After 7 days from weaning, these plants were transferred to a shade house to grow and set flowers. Water spray containing 0.50 μg of NPK (1:1:1) was given at intervals of 4 d to enhance success rate of acclimatised plants. Success rates of 96 and 80% were noted on plumule and radicle-induced plants, respectively. It is further concluded that use of this novel methodology would favorably facilitate its use in genetic transformation and functional genomic studies.
format	Article
author	Kahriz, Parisa Pourali Kahriz, Mahsa Pourali
spellingShingle	Kahriz, Parisa Pourali Kahriz, Mahsa Pourali Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds
author_facet	Kahriz, Parisa Pourali Kahriz, Mahsa Pourali
author_sort	Kahriz, Parisa Pourali
title	Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds
title_short	Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds
title_full	Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds
title_fullStr	Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds
title_full_unstemmed	Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds
title_sort	somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (triticum aestivum l.) cv. kunduru and cv. cakmak seeds
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/10590/ http://journalarticle.ukm.my/10590/ http://journalarticle.ukm.my/10590/1/05%20Parisa%20Pourali%20Kahriz.pdf
first_indexed	2023-09-18T19:57:54Z
last_indexed	2023-09-18T19:57:54Z
_version_	1777406652280995840

Somatic embryogenesis on plumule and radicle explants obtained from warm water hydroprimed wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

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