Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance

The present study was conducted to determine the prevalence and antibiotic resistance of Salmonella sp. isolated from African catfish (Clarias gariepinus). A total of 30 catfish were harvested from four different farms and four different wet markets. A total of 60 samples (30 catfish skins and 30 ca...

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Main Authors:	Chia, Kim Sing, Md. Zahirul Islam Khan, Hassan Hj. Mohd Daud, Abd Rahman Aziz
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/10376/ http://journalarticle.ukm.my/10376/ http://journalarticle.ukm.my/10376/1/02%20Chia%20Kim%20Seng.pdf

id	ukm-10376
recordtype	eprints
spelling	ukm-103762017-05-21T23:54:13Z http://journalarticle.ukm.my/10376/ Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance Chia, Kim Sing Md. Zahirul Islam Khan, Hassan Hj. Mohd Daud, Abd Rahman Aziz, The present study was conducted to determine the prevalence and antibiotic resistance of Salmonella sp. isolated from African catfish (Clarias gariepinus). A total of 30 catfish were harvested from four different farms and four different wet markets. A total of 60 samples (30 catfish skins and 30 catfish intestines) were used for Salmonella sp. isolation (pellet-method), its biochemical and serological test. Confirmation of Salmonella sp. were determined by polyvalent O antisera and polymerase chain reaction (PCR) using genus specific primers for invA genes (DNA amplification showed one distinct band with molecular weight of 389 bp) and the species of isolated Salmonella sp. were identified by serotyping. The result showed 6/30 (20%) of fish or 6/60 (10%) of organ samples were positive for Salmonella sp. Among those positive for Salmonella sp., 4/6 were from intestine samples and 2/6 were from skin samples. No significant difference was found in the prevalence of Salmonella sp. isolates between fish harvested from farms and wet markets (p-value= 0.406). The Salmonella serovars identified were Salmonella corvallis (n=3), Salmonella mbandaka (n=2) and Salmonella typhmurium (n=1). Salmonella sp. isolates were resistance to Penicillin (P 10, 100%), Clindamycin (DA 2, 100%), Tetracycline (TE 30, 100%) and Rifampicin (RD 5, 100%) and all of the isolates were susceptible or intermediate resistance to Ceftazidime (CAZ 30) and Trimethopin (W 5). Multiple antibiotic resistance (MAR) index of all Salmonella sp. isolates in current study was 0.67 indicating that fish sampled in the present study was under high risk of been exposed to the tested antibiotics. Penerbit Universiti Kebangsaan Malaysia 2016-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10376/1/02%20Chia%20Kim%20Seng.pdf Chia, Kim Sing and Md. Zahirul Islam Khan, and Hassan Hj. Mohd Daud, and Abd Rahman Aziz, (2016) Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance. Sains Malaysiana, 45 (11). pp. 1597-1602. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil11_2016/KandunganJilid45Bil11_2016.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The present study was conducted to determine the prevalence and antibiotic resistance of Salmonella sp. isolated from African catfish (Clarias gariepinus). A total of 30 catfish were harvested from four different farms and four different wet markets. A total of 60 samples (30 catfish skins and 30 catfish intestines) were used for Salmonella sp. isolation (pellet-method), its biochemical and serological test. Confirmation of Salmonella sp. were determined by polyvalent O antisera and polymerase chain reaction (PCR) using genus specific primers for invA genes (DNA amplification showed one distinct band with molecular weight of 389 bp) and the species of isolated Salmonella sp. were identified by serotyping. The result showed 6/30 (20%) of fish or 6/60 (10%) of organ samples were positive for Salmonella sp. Among those positive for Salmonella sp., 4/6 were from intestine samples and 2/6 were from skin samples. No significant difference was found in the prevalence of Salmonella sp. isolates between fish harvested from farms and wet markets (p-value= 0.406). The Salmonella serovars identified were Salmonella corvallis (n=3), Salmonella mbandaka (n=2) and Salmonella typhmurium (n=1). Salmonella sp. isolates were resistance to Penicillin (P 10, 100%), Clindamycin (DA 2, 100%), Tetracycline (TE 30, 100%) and Rifampicin (RD 5, 100%) and all of the isolates were susceptible or intermediate resistance to Ceftazidime (CAZ 30) and Trimethopin (W 5). Multiple antibiotic resistance (MAR) index of all Salmonella sp. isolates in current study was 0.67 indicating that fish sampled in the present study was under high risk of been exposed to the tested antibiotics.
format	Article
author	Chia, Kim Sing Md. Zahirul Islam Khan, Hassan Hj. Mohd Daud, Abd Rahman Aziz,
spellingShingle	Chia, Kim Sing Md. Zahirul Islam Khan, Hassan Hj. Mohd Daud, Abd Rahman Aziz, Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance
author_facet	Chia, Kim Sing Md. Zahirul Islam Khan, Hassan Hj. Mohd Daud, Abd Rahman Aziz,
author_sort	Chia, Kim Sing
title	Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance
title_short	Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance
title_full	Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance
title_fullStr	Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance
title_full_unstemmed	Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance
title_sort	prevalence of salmonella sp. in african catfish (clarias gariepinus) obtained from farms and wet markets in kelantan, malaysia and their antibiotic resistance
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/10376/ http://journalarticle.ukm.my/10376/ http://journalarticle.ukm.my/10376/1/02%20Chia%20Kim%20Seng.pdf
first_indexed	2023-09-18T19:57:15Z
last_indexed	2023-09-18T19:57:15Z
_version_	1777406612172963840

Prevalence of Salmonella sp. in African Catfish (Clarias gariepinus) obtained from farms and wet markets in Kelantan, Malaysia and their antibiotic resistance

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