Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates

Sea cucumber (Stichopus horrens) protein hydrolysates were known as a potential functional food source with angiotensinconverting enzyme (ACE) inhibitory. The genotoxicity properties of S. horrens protein hydrolysates have been investigated through two different in vitro tests: Ames test and in vi...

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Main Authors:	Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/1/44_1_01.pdf

id	ukm-10335
recordtype	eprints
spelling	ukm-103352017-04-21T09:19:10Z http://journalarticle.ukm.my/10335/ Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah, Sea cucumber (Stichopus horrens) protein hydrolysates were known as a potential functional food source with angiotensinconverting enzyme (ACE) inhibitory. The genotoxicity properties of S. horrens protein hydrolysates have been investigated through two different in vitro tests: Ames test and in vitro micronucleus test while the cytotoxicity properties of S. horrens protein hydrolysate were assessed using neutral red test. The study was conducted at a concentration up to 8000 μg/ml, 80 μg/ml and 50 μg/ml for Ames test, in vitro micronucleus test and neutral red test respectively with and without metabolic activation. There were no increments in the number of revertant colonies observed at any concentrations of S. horrens protein hydrolysates with and without metabolic activation in all four strains of Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) compared to the solvent control. In in vitro micronucleus test, S. horrens protein hydrolysate did not induce clastogenicity in V79 cell while in neutral red test, S. horrens protein hydrolysate did not show any cytotoxic effects on NIH/3T3 mouse fibroblast cell. In conclusion, S. horrens protein hydrolysates are safe in terms of genotoxic and cytotoxic hence have the potential to be used in pharmaceutical and food industries as functional ingredient. Penerbit Universiti Kebangsaan Malaysia 2015-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10335/1/44_1_01.pdf Obren James Kawanding, and Noriham Abdullah, and Zainon Mohd Noor, and Nooraain Hashim, and Nazamid Saari, and Mohd Fakharul Zaman Raja Yahya, and Mohd Faiz Foong Abdullah, (2015) Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates. Malaysian Applied Biology, 44 (3). pp. 1-4. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=520&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Sea cucumber (Stichopus horrens) protein hydrolysates were known as a potential functional food source with angiotensinconverting enzyme (ACE) inhibitory. The genotoxicity properties of S. horrens protein hydrolysates have been investigated through two different in vitro tests: Ames test and in vitro micronucleus test while the cytotoxicity properties of S. horrens protein hydrolysate were assessed using neutral red test. The study was conducted at a concentration up to 8000 μg/ml, 80 μg/ml and 50 μg/ml for Ames test, in vitro micronucleus test and neutral red test respectively with and without metabolic activation. There were no increments in the number of revertant colonies observed at any concentrations of S. horrens protein hydrolysates with and without metabolic activation in all four strains of Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) compared to the solvent control. In in vitro micronucleus test, S. horrens protein hydrolysate did not induce clastogenicity in V79 cell while in neutral red test, S. horrens protein hydrolysate did not show any cytotoxic effects on NIH/3T3 mouse fibroblast cell. In conclusion, S. horrens protein hydrolysates are safe in terms of genotoxic and cytotoxic hence have the potential to be used in pharmaceutical and food industries as functional ingredient.
format	Article
author	Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah,
spellingShingle	Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah, Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates
author_facet	Obren James Kawanding, Noriham Abdullah, Zainon Mohd Noor, Nooraain Hashim, Nazamid Saari, Mohd Fakharul Zaman Raja Yahya, Mohd Faiz Foong Abdullah,
author_sort	Obren James Kawanding,
title	Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates
title_short	Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates
title_full	Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates
title_fullStr	Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates
title_full_unstemmed	Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates
title_sort	genotoxicity and cytotoxicity evaluation of sea cucumber (stichopus horrens) protein hydrolysates
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/1/44_1_01.pdf
first_indexed	2023-09-18T19:57:07Z
last_indexed	2023-09-18T19:57:07Z
_version_	1777406603535843328

Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates

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