Genotoxicity and cytotoxicity evaluation of sea cucumber (Stichopus horrens) protein hydrolysates
Sea cucumber (Stichopus horrens) protein hydrolysates were known as a potential functional food source with angiotensinconverting enzyme (ACE) inhibitory. The genotoxicity properties of S. horrens protein hydrolysates have been investigated through two different in vitro tests: Ames test and in vi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2015
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| Online Access: | http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/ http://journalarticle.ukm.my/10335/1/44_1_01.pdf |
| Summary: | Sea cucumber (Stichopus horrens) protein hydrolysates were known as a potential functional food source with angiotensinconverting
enzyme (ACE) inhibitory. The genotoxicity properties of S. horrens protein hydrolysates have been investigated
through two different in vitro tests: Ames test and in vitro micronucleus test while the cytotoxicity properties of S. horrens
protein hydrolysate were assessed using neutral red test. The study was conducted at a concentration up to 8000 μg/ml, 80
μg/ml and 50 μg/ml for Ames test, in vitro micronucleus test and neutral red test respectively with and without metabolic
activation. There were no increments in the number of revertant colonies observed at any concentrations of S. horrens protein
hydrolysates with and without metabolic activation in all four strains of Salmonella typhimurium (TA98, TA100, TA1535
and TA1537) compared to the solvent control. In in vitro micronucleus test, S. horrens protein hydrolysate did not induce
clastogenicity in V79 cell while in neutral red test, S. horrens protein hydrolysate did not show any cytotoxic effects on
NIH/3T3 mouse fibroblast cell. In conclusion, S. horrens protein hydrolysates are safe in terms of genotoxic and cytotoxic
hence have the potential to be used in pharmaceutical and food industries as functional ingredient. |
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