Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants

Polyhydroxybutyrate (PHB) otherwise known as bioplastics are biodegradable materials that are accumulated in various microorganisms to serve as carbon and energy reservoirs and regarded as an attractive alternative to petroleum-derived plastics. Although research has been conducted on isolation of P...

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Main Authors:	Haliru Musa, Bolanle, Badmus Basirat, Farizul Hafiz Kasim, Dachyar Arbain
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/10291/ http://journalarticle.ukm.my/10291/ http://journalarticle.ukm.my/10291/1/07%20Haliru%20Musa.pdf

id	ukm-10291
recordtype	eprints
spelling	ukm-102912017-04-06T09:04:43Z http://journalarticle.ukm.my/10291/ Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants Haliru Musa, Bolanle, Badmus Basirat Farizul Hafiz Kasim, Dachyar Arbain, Polyhydroxybutyrate (PHB) otherwise known as bioplastics are biodegradable materials that are accumulated in various microorganisms to serve as carbon and energy reservoirs and regarded as an attractive alternative to petroleum-derived plastics. Although research has been conducted on isolation of PHB-producing microorganisms from different ecological environments, few studies have been carried out on isolation of potential PHB-producing microorganisms from rhizosphere environment of groundnut plants, Arachis hypogaea which can be regarded as a good environment for the isolation of potential PHB-producing microorganisms. In the present study, a total of twenty-one (21) bacterial strains were primarily screened and isolated from rhizosphere soil of a groundnut plant. Four bacterial isolates with maximum PHB-producing potential upon screening using submerged fermentation were selected for further studies. The fermentation pattern of PHB production was studied using different nutrient sources. The influence of agitation on PHB production was also studied. Mannitol stimulated maximum (6.076a mg/mL) PHB production by Bacillus sp. 1; KNO3 used as a limiting nutrient induced best (5.728a mg/mL) PHB production by Citrobacter sp. and MgSO4.7H2O supported maximum (5.972a mg/mL) PHB production in Enterococcus sp. A low agitation speed of 150 rpm was found to support best (5.802a mg/mL) PHB production by Bacillus sp.1. Findings from this study indicated that the isolated bacterial strains have high PHB- producing potential. The need to explore other environment harbouring microbial strains with high PHB-producing potential is paramount to the discovery of bioplastics with improved properties for potential industrial applications. Penerbit Universiti Kebangsaan Malaysia 2016-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10291/1/07%20Haliru%20Musa.pdf Haliru Musa, and Bolanle, Badmus Basirat and Farizul Hafiz Kasim, and Dachyar Arbain, (2016) Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants. Sains Malaysiana, 45 (10). pp. 1469-1476. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num10_2016/contentsVol45num10_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Polyhydroxybutyrate (PHB) otherwise known as bioplastics are biodegradable materials that are accumulated in various microorganisms to serve as carbon and energy reservoirs and regarded as an attractive alternative to petroleum-derived plastics. Although research has been conducted on isolation of PHB-producing microorganisms from different ecological environments, few studies have been carried out on isolation of potential PHB-producing microorganisms from rhizosphere environment of groundnut plants, Arachis hypogaea which can be regarded as a good environment for the isolation of potential PHB-producing microorganisms. In the present study, a total of twenty-one (21) bacterial strains were primarily screened and isolated from rhizosphere soil of a groundnut plant. Four bacterial isolates with maximum PHB-producing potential upon screening using submerged fermentation were selected for further studies. The fermentation pattern of PHB production was studied using different nutrient sources. The influence of agitation on PHB production was also studied. Mannitol stimulated maximum (6.076a mg/mL) PHB production by Bacillus sp. 1; KNO3 used as a limiting nutrient induced best (5.728a mg/mL) PHB production by Citrobacter sp. and MgSO4.7H2O supported maximum (5.972a mg/mL) PHB production in Enterococcus sp. A low agitation speed of 150 rpm was found to support best (5.802a mg/mL) PHB production by Bacillus sp.1. Findings from this study indicated that the isolated bacterial strains have high PHB- producing potential. The need to explore other environment harbouring microbial strains with high PHB-producing potential is paramount to the discovery of bioplastics with improved properties for potential industrial applications.
format	Article
author	Haliru Musa, Bolanle, Badmus Basirat Farizul Hafiz Kasim, Dachyar Arbain,
spellingShingle	Haliru Musa, Bolanle, Badmus Basirat Farizul Hafiz Kasim, Dachyar Arbain, Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants
author_facet	Haliru Musa, Bolanle, Badmus Basirat Farizul Hafiz Kasim, Dachyar Arbain,
author_sort	Haliru Musa,
title	Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants
title_short	Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants
title_full	Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants
title_fullStr	Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants
title_full_unstemmed	Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants
title_sort	screening and production of polyhydroxybutyrate (phb) by bacterial strains isolated from rhizosphere soil of groundnut plants
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/10291/ http://journalarticle.ukm.my/10291/ http://journalarticle.ukm.my/10291/1/07%20Haliru%20Musa.pdf
first_indexed	2023-09-18T19:57:01Z
last_indexed	2023-09-18T19:57:01Z
_version_	1777406597116461056

Screening and production of polyhydroxybutyrate (PHB) by bacterial strains isolated from rhizosphere soil of groundnut plants

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