Phytochemical and biological evaluation of defatted seeds of Jatropha curcas

In this study, the antimicrobial, antioxidant, phytotoxic and phytochemical properties of defatted seeds of Jatropha curcas were evaluated. A crude methanolic extract of defatted seeds was tested against three fungal strains - Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus - and fiv...

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Main Authors:	ul Haq, Muhammad Nisar, Wazir, Sultan Mehmood, Ullah, Faizan, Khan, Rahmat Ali, Shah, Mir Sadiq, Khatak, Adnan
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/10287/ http://journalarticle.ukm.my/10287/ http://journalarticle.ukm.my/10287/1/03%20Muhammad%20Nisar.pdf

id	ukm-10287
recordtype	eprints
spelling	ukm-102872017-04-06T08:47:37Z http://journalarticle.ukm.my/10287/ Phytochemical and biological evaluation of defatted seeds of Jatropha curcas ul Haq, Muhammad Nisar Wazir, Sultan Mehmood Ullah, Faizan Khan, Rahmat Ali Shah, Mir Sadiq Khatak, Adnan In this study, the antimicrobial, antioxidant, phytotoxic and phytochemical properties of defatted seeds of Jatropha curcas were evaluated. A crude methanolic extract of defatted seeds was tested against three fungal strains - Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus - and five bacteria: Escherichia coli and Klebsiella pneumoniae (Gram-negative) and Micrococcus luteus, Bacillus subtilis and Staphylococcus aureus (Gram positive). The methanolic extract was diluted in dimethylsulfoxide to final concentrations of 1, 2, 3, 4 and 5 mg/10 mL. The largest zones of inhibition against K. pneumoniae, M. luteus and B. subtilis were achieved using the concentration of 5 mg/10 mL. The concentration of 1 mg/10 mL was most effective against S. aureus and E. coli. In a 1, 1-diphenyl-2-picrylahydrazyl (DPPH) radical scavenging assay, the 5 mg/10 mL concentration of the Jatropha seed extract showed the strongest activity. Higher concentrations of the Jatropha seed extract (10 mg/50 mL and 5 mg/50 mL) significantly inhibited the germination of radish seeds and had negative effects on radish seedling relative water content, shoot length, root length, seedling fresh weight and seedling dry weight (p<0.05). Phytochemical analyses of the defatted seeds detected alkaloids (7.3%), flavonoids (0.39%) and soluble phenolics (mg gallic acid equivalents/g extract). Based on these results, it was inferred that J. curcas seeds contain active ingredients that are effective against pathogenic microbes and therefore could be used to formulate drugs to treat various diseases. Penerbit Universiti Kebangsaan Malaysia 2016-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10287/1/03%20Muhammad%20Nisar.pdf ul Haq, Muhammad Nisar and Wazir, Sultan Mehmood and Ullah, Faizan and Khan, Rahmat Ali and Shah, Mir Sadiq and Khatak, Adnan (2016) Phytochemical and biological evaluation of defatted seeds of Jatropha curcas. Sains Malaysiana, 45 (10). pp. 1435-1442. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num10_2016/contentsVol45num10_2016.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In this study, the antimicrobial, antioxidant, phytotoxic and phytochemical properties of defatted seeds of Jatropha curcas were evaluated. A crude methanolic extract of defatted seeds was tested against three fungal strains - Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus - and five bacteria: Escherichia coli and Klebsiella pneumoniae (Gram-negative) and Micrococcus luteus, Bacillus subtilis and Staphylococcus aureus (Gram positive). The methanolic extract was diluted in dimethylsulfoxide to final concentrations of 1, 2, 3, 4 and 5 mg/10 mL. The largest zones of inhibition against K. pneumoniae, M. luteus and B. subtilis were achieved using the concentration of 5 mg/10 mL. The concentration of 1 mg/10 mL was most effective against S. aureus and E. coli. In a 1, 1-diphenyl-2-picrylahydrazyl (DPPH) radical scavenging assay, the 5 mg/10 mL concentration of the Jatropha seed extract showed the strongest activity. Higher concentrations of the Jatropha seed extract (10 mg/50 mL and 5 mg/50 mL) significantly inhibited the germination of radish seeds and had negative effects on radish seedling relative water content, shoot length, root length, seedling fresh weight and seedling dry weight (p<0.05). Phytochemical analyses of the defatted seeds detected alkaloids (7.3%), flavonoids (0.39%) and soluble phenolics (mg gallic acid equivalents/g extract). Based on these results, it was inferred that J. curcas seeds contain active ingredients that are effective against pathogenic microbes and therefore could be used to formulate drugs to treat various diseases.
format	Article
author	ul Haq, Muhammad Nisar Wazir, Sultan Mehmood Ullah, Faizan Khan, Rahmat Ali Shah, Mir Sadiq Khatak, Adnan
spellingShingle	ul Haq, Muhammad Nisar Wazir, Sultan Mehmood Ullah, Faizan Khan, Rahmat Ali Shah, Mir Sadiq Khatak, Adnan Phytochemical and biological evaluation of defatted seeds of Jatropha curcas
author_facet	ul Haq, Muhammad Nisar Wazir, Sultan Mehmood Ullah, Faizan Khan, Rahmat Ali Shah, Mir Sadiq Khatak, Adnan
author_sort	ul Haq, Muhammad Nisar
title	Phytochemical and biological evaluation of defatted seeds of Jatropha curcas
title_short	Phytochemical and biological evaluation of defatted seeds of Jatropha curcas
title_full	Phytochemical and biological evaluation of defatted seeds of Jatropha curcas
title_fullStr	Phytochemical and biological evaluation of defatted seeds of Jatropha curcas
title_full_unstemmed	Phytochemical and biological evaluation of defatted seeds of Jatropha curcas
title_sort	phytochemical and biological evaluation of defatted seeds of jatropha curcas
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/10287/ http://journalarticle.ukm.my/10287/ http://journalarticle.ukm.my/10287/1/03%20Muhammad%20Nisar.pdf
first_indexed	2023-09-18T19:57:00Z
last_indexed	2023-09-18T19:57:00Z
_version_	1777406596526112768

Phytochemical and biological evaluation of defatted seeds of Jatropha curcas

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