Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala

Natural peptides in insect vectors played an important role in the control of pathogens. Musca domestica Linnaeus and Chrysomya megacephala Fabricius were two species of local fly chosen to detect presence of antimicrobial peptide substance. The screening of the antimicrobial activity was carried us...

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Main Authors:	Ahmad Zorin Sahalan, Baharudin Omar, Aima Yusirah Mohamed, John Jeffery
Format:	Article
Language:	English
Published:	penerbit ukm 2006
Online Access:	http://journalarticle.ukm.my/1023/ http://journalarticle.ukm.my/1023/ http://journalarticle.ukm.my/1023/1/jurnal58.pdf

id	ukm-1023
recordtype	eprints
spelling	ukm-10232016-12-14T06:28:36Z http://journalarticle.ukm.my/1023/ Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala Ahmad Zorin Sahalan, Baharudin Omar, Aima Yusirah Mohamed, John Jeffery, Natural peptides in insect vectors played an important role in the control of pathogens. Musca domestica Linnaeus and Chrysomya megacephala Fabricius were two species of local fly chosen to detect presence of antimicrobial peptide substance. The screening of the antimicrobial activity was carried using a spectrophotometric method. Results were obtained much quicker and less laborious. The results showed larva hemolymph of M. domestica lysed Bacillus subtilis and two Gram negatives, Escherichia coli and Pseudomona. aeruginosa. The pupae hemolymph only lysed E.coli. Whereas, the hemolymph of C. megacephala larva showed bactericidal effect against both of the Gram positives tested, i.e. B. subtilis and Staph. aureus. and no effect was against the Gram negatives. The pupa showed lytic activity against Staph. aureus and P. aeruginosa. As a conclusion, the larva and pupa hemolymph of M. domestica and C. megacephala demonstrated antibacterial activity. However, larva hemolymph of M. domestica and C. megacephala has broader antibacterial activity against both Gram positive and negative bacteria. penerbit ukm 2006 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/1023/1/jurnal58.pdf Ahmad Zorin Sahalan, and Baharudin Omar, and Aima Yusirah Mohamed, and John Jeffery, (2006) Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala. Jurnal Sains Kesihatan Malaysia, 4 (2). pp. 1-11. ISSN 1675-8161 http://www.fskb.ukm.my/fskbold/jurnal
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Natural peptides in insect vectors played an important role in the control of pathogens. Musca domestica Linnaeus and Chrysomya megacephala Fabricius were two species of local fly chosen to detect presence of antimicrobial peptide substance. The screening of the antimicrobial activity was carried using a spectrophotometric method. Results were obtained much quicker and less laborious. The results showed larva hemolymph of M. domestica lysed Bacillus subtilis and two Gram negatives, Escherichia coli and Pseudomona. aeruginosa. The pupae hemolymph only lysed E.coli. Whereas, the hemolymph of C. megacephala larva showed bactericidal effect against both of the Gram positives tested, i.e. B. subtilis and Staph. aureus. and no effect was against the Gram negatives. The pupa showed lytic activity against Staph. aureus and P. aeruginosa. As a conclusion, the larva and pupa hemolymph of M. domestica and C. megacephala demonstrated antibacterial activity. However, larva hemolymph of M. domestica and C. megacephala has broader antibacterial activity against both Gram positive and negative bacteria.
format	Article
author	Ahmad Zorin Sahalan, Baharudin Omar, Aima Yusirah Mohamed, John Jeffery,
spellingShingle	Ahmad Zorin Sahalan, Baharudin Omar, Aima Yusirah Mohamed, John Jeffery, Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala
author_facet	Ahmad Zorin Sahalan, Baharudin Omar, Aima Yusirah Mohamed, John Jeffery,
author_sort	Ahmad Zorin Sahalan,
title	Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala
title_short	Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala
title_full	Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala
title_fullStr	Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala
title_full_unstemmed	Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala
title_sort	antibacterial activity of extracted hemolymph from larvae and pupae of local fly species musca domestica and chrysomya megacephala
publisher	penerbit ukm
publishDate	2006
url	http://journalarticle.ukm.my/1023/ http://journalarticle.ukm.my/1023/ http://journalarticle.ukm.my/1023/1/jurnal58.pdf
first_indexed	2023-09-18T19:32:08Z
last_indexed	2023-09-18T19:32:08Z
_version_	1777405031528529920

Antibacterial activity of extracted Hemolymph from larvae and pupae of local fly species Musca Domestica and Chrysomya Megacephala

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