Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells

Hibiscus sabdariffa Linn. (roselle) is a polyphenol rich fruit. This study aimed to identify the neuroprotective effect of roselle on LPS-induced cell proliferation and nitric oxide-induced free radical in microglia and neuroblastoma cells. MTT assay was used to identify the appropriate concentratio...

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Main Authors:	Nor Fadilah Rajab, Siti Masitah Musa, Munirah Ahmad Munawar, Leong, Lek Mun, Heng, Kai Yen, Farah Wahida Ibrahim, Chan, Kok Meng
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:	http://journalarticle.ukm.my/10121/ http://journalarticle.ukm.my/10121/ http://journalarticle.ukm.my/10121/1/14739-40623-1-SM.pdf

id	ukm-10121
recordtype	eprints
spelling	ukm-101212017-02-20T01:33:58Z http://journalarticle.ukm.my/10121/ Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells Nor Fadilah Rajab, Siti Masitah Musa, Munirah Ahmad Munawar, Leong, Lek Mun Heng, Kai Yen Farah Wahida Ibrahim, Chan, Kok Meng Hibiscus sabdariffa Linn. (roselle) is a polyphenol rich fruit. This study aimed to identify the neuroprotective effect of roselle on LPS-induced cell proliferation and nitric oxide-induced free radical in microglia and neuroblastoma cells. MTT assay was used to identify the appropriate concentration of roselle and LPS for microglia and neuroblastoma cells proliferation study. Griess assay were used to determine the level of nitric oxide accumulated based on the reaction of Griess to estimate the activity of iNOS in nitric oxide production. The results showed that roselle at the concentration of 50 μg/mL and 100 μg/mL and LPS at concentration of 1 μg/mL does not give cytotoxic effect towards microglia C8-B4 and neuroblastoma LN18 cells. The roselle treatment at 50 μg/mL and 100 μg/mL showed a protective effect on LPS-induced microglia C8-B4 cells. However, in neuroblastoma LN18 cells, no protective effect was seen on both 50 μg/mL and 100 μg/mL of roselle treatment following induction with 1 μg/mL of LPS. On the other hand, the production of nitric oxide (NO) was reduced when LPS-induced microglia C8-B4 cells were treated with 50 μg/mL of roselle. Treatment of roselle at concentration 100 μg/mL on LPS-induced neuroblastoma LN18 cells also reduced the production of nitric oxide. As a conclusion, roselle had the ability to give neuroprotective effect by the inhibition of LPS induction activity on microglia activation for normal and cancer cells at different concentrations. Penerbit Universiti Kebangsaan Malaysia 2016 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/10121/1/14739-40623-1-SM.pdf Nor Fadilah Rajab, and Siti Masitah Musa, and Munirah Ahmad Munawar, and Leong, Lek Mun and Heng, Kai Yen and Farah Wahida Ibrahim, and Chan, Kok Meng (2016) Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells. Jurnal Sains Kesihatan Malaysia, 14 (2). pp. 111-117. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/634
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Hibiscus sabdariffa Linn. (roselle) is a polyphenol rich fruit. This study aimed to identify the neuroprotective effect of roselle on LPS-induced cell proliferation and nitric oxide-induced free radical in microglia and neuroblastoma cells. MTT assay was used to identify the appropriate concentration of roselle and LPS for microglia and neuroblastoma cells proliferation study. Griess assay were used to determine the level of nitric oxide accumulated based on the reaction of Griess to estimate the activity of iNOS in nitric oxide production. The results showed that roselle at the concentration of 50 μg/mL and 100 μg/mL and LPS at concentration of 1 μg/mL does not give cytotoxic effect towards microglia C8-B4 and neuroblastoma LN18 cells. The roselle treatment at 50 μg/mL and 100 μg/mL showed a protective effect on LPS-induced microglia C8-B4 cells. However, in neuroblastoma LN18 cells, no protective effect was seen on both 50 μg/mL and 100 μg/mL of roselle treatment following induction with 1 μg/mL of LPS. On the other hand, the production of nitric oxide (NO) was reduced when LPS-induced microglia C8-B4 cells were treated with 50 μg/mL of roselle. Treatment of roselle at concentration 100 μg/mL on LPS-induced neuroblastoma LN18 cells also reduced the production of nitric oxide. As a conclusion, roselle had the ability to give neuroprotective effect by the inhibition of LPS induction activity on microglia activation for normal and cancer cells at different concentrations.
format	Article
author	Nor Fadilah Rajab, Siti Masitah Musa, Munirah Ahmad Munawar, Leong, Lek Mun Heng, Kai Yen Farah Wahida Ibrahim, Chan, Kok Meng
spellingShingle	Nor Fadilah Rajab, Siti Masitah Musa, Munirah Ahmad Munawar, Leong, Lek Mun Heng, Kai Yen Farah Wahida Ibrahim, Chan, Kok Meng Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells
author_facet	Nor Fadilah Rajab, Siti Masitah Musa, Munirah Ahmad Munawar, Leong, Lek Mun Heng, Kai Yen Farah Wahida Ibrahim, Chan, Kok Meng
author_sort	Nor Fadilah Rajab,
title	Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells
title_short	Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells
title_full	Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells
title_fullStr	Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells
title_full_unstemmed	Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells
title_sort	anti-neuroinflammatory effects of hibiscus sabdariffa linn. (roselle) on lipopolysaccharides-induced microglia and neuroblastoma cells
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2016
url	http://journalarticle.ukm.my/10121/ http://journalarticle.ukm.my/10121/ http://journalarticle.ukm.my/10121/1/14739-40623-1-SM.pdf
first_indexed	2023-09-18T19:56:34Z
last_indexed	2023-09-18T19:56:34Z
_version_	1777406568882503680

Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells

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