In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli

In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli

Thromboembolic is a disorder which rose up from the abnormal haemostasis process. Most of anticoagulant drug used in the treatment and prevention of thromboembolic disorder caused side effects such as bleeding. Potential of Rhodomyrtus tomentosa as a native anticoagulant been tested by evaluating th...

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Main Author: Adli, Norizzaty
Format: Thesis
Language:English
Published: 2015
Subjects:
Clinical pathology. Laboratory technique
Online Access:http://ir.uitm.edu.my/id/eprint/28053/
http://ir.uitm.edu.my/id/eprint/28053/1/TD_NORIZZATY%20ADLI%20HS%2015_5.pdf
id uitm-28053
recordtype eprints
spelling uitm-280532020-02-03T04:40:59Z http://ir.uitm.edu.my/id/eprint/28053/ In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli Adli, Norizzaty Clinical pathology. Laboratory technique Thromboembolic is a disorder which rose up from the abnormal haemostasis process. Most of anticoagulant drug used in the treatment and prevention of thromboembolic disorder caused side effects such as bleeding. Potential of Rhodomyrtus tomentosa as a native anticoagulant been tested by evaluating the possible haemostatic effect of Rhodomyrtus tomentosa aqueous leaf extract in vitro. In vitro coagulation assays such as Thrombin time (TT), Prothrombin time (PT) and Activated partial thromboplastin time (APTT) tests were performed on normal coagulation control plasma spiked with different concentrations of the aqueous leaf extract (10 - 100%). Total tannin and flavonoid for all different concentrations of leaf extract were measured using spectrophotometer and correlate with clotting times (TT, PT and APTT). The results were analyzed using one way ANOVA followed by post-hoc Dunnet's and Pearson's correlation. The results showed that TT, PT and APTT of normal coagulation control plasma spiked with extract were markedly prolonged. A significant anticoagulant (p < 0.05) showed by PT was at lowest concentration (10%), TT was at concentration 50 - 100% and APTT was at concentration 80% 100%. The aqueous extraction showed a better anticoagulant for PT. The clotting times for TT, PT and APTT were significantly correlated (p < 0.05) with total tannin and flavonoid. Besides, the total tannin and flavonoid was significantly decreased (p < 0.05) when exposed to the light for 5 days. In conclusion, the results highlights that Rhodomyrtus tomentosa contain anticoagulant properties reflected the bioactive compound of tannin and flavonoid. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/28053/1/TD_NORIZZATY%20ADLI%20HS%2015_5.pdf Adli, Norizzaty (2015) In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli. Degree thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Clinical pathology. Laboratory technique
spellingShingle Clinical pathology. Laboratory technique
Adli, Norizzaty
In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
description Thromboembolic is a disorder which rose up from the abnormal haemostasis process. Most of anticoagulant drug used in the treatment and prevention of thromboembolic disorder caused side effects such as bleeding. Potential of Rhodomyrtus tomentosa as a native anticoagulant been tested by evaluating the possible haemostatic effect of Rhodomyrtus tomentosa aqueous leaf extract in vitro. In vitro coagulation assays such as Thrombin time (TT), Prothrombin time (PT) and Activated partial thromboplastin time (APTT) tests were performed on normal coagulation control plasma spiked with different concentrations of the aqueous leaf extract (10 - 100%). Total tannin and flavonoid for all different concentrations of leaf extract were measured using spectrophotometer and correlate with clotting times (TT, PT and APTT). The results were analyzed using one way ANOVA followed by post-hoc Dunnet's and Pearson's correlation. The results showed that TT, PT and APTT of normal coagulation control plasma spiked with extract were markedly prolonged. A significant anticoagulant (p < 0.05) showed by PT was at lowest concentration (10%), TT was at concentration 50 - 100% and APTT was at concentration 80% 100%. The aqueous extraction showed a better anticoagulant for PT. The clotting times for TT, PT and APTT were significantly correlated (p < 0.05) with total tannin and flavonoid. Besides, the total tannin and flavonoid was significantly decreased (p < 0.05) when exposed to the light for 5 days. In conclusion, the results highlights that Rhodomyrtus tomentosa contain anticoagulant properties reflected the bioactive compound of tannin and flavonoid.
format Thesis
author Adli, Norizzaty
author_facet Adli, Norizzaty
author_sort Adli, Norizzaty
title In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_short In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_full In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_fullStr In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_full_unstemmed In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_sort in vitro haemostatic activity of rhodomyrtus tomentosa (aiton) hassk. aqueous leaf extract / norizzaty adli
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/28053/
http://ir.uitm.edu.my/id/eprint/28053/1/TD_NORIZZATY%20ADLI%20HS%2015_5.pdf
first_indexed 2023-09-18T23:19:30Z
last_indexed 2023-09-18T23:19:30Z
_version_ 1777419336698298368

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