Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor

DEL phenotype is known as the weakest D antigen in the Rh blood group system. Serologically, DEL phenotype commonly mistyped as true Rh negative. An anti-D immunization may occurred in Rh negative recipient who received blood from the DEL phenotype donor. The goals of the present study were to detec...

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Main Author:	Che Mohd Noor, Nor Arina
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Clinical pathology. Laboratory technique Examination of the blood
Online Access:	http://ir.uitm.edu.my/id/eprint/28026/ http://ir.uitm.edu.my/id/eprint/28026/1/TD_NOR%20ARINA%20CHE%20MOHD%20NOOR%20HS%2015_5.pdf

id	uitm-28026
recordtype	eprints
spelling	uitm-280262020-02-04T08:57:25Z http://ir.uitm.edu.my/id/eprint/28026/ Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor Che Mohd Noor, Nor Arina Clinical pathology. Laboratory technique Examination of the blood DEL phenotype is known as the weakest D antigen in the Rh blood group system. Serologically, DEL phenotype commonly mistyped as true Rh negative. An anti-D immunization may occurred in Rh negative recipient who received blood from the DEL phenotype donor. The goals of the present study were to detect DEL phenotype in Rh negative blood by using chloroform elution technique and specific sequence primer-polymerase chain reaction (SSP-PCR). A total of 43 Rh negative blood donor's samples from National Blood Centre were examined for the Rh phenotyping and the presence of DEL phenotype by using chloroform elution technique and SSPPCR. The Rh negative phenotypes consisted of 79.07% dce, 13.95% dCce, 4.65% dcEe and 2.33% dCe. Chloroform elution technique failed to detect DEL phenotype, but only 2.33% (1/43) Rh negative blood donor's sample was being detected as DEL phenotype by using SSP-PCR. In conclusion, based on these findings, chloroform elution technique might not be an effective method in detection of DEL phenotype. Sensitivity and specificity of molecular typing method can overcome the limitation of serological method in the clinical laboratory. Hence, it is highly recommended to use SSP-PCR in detection of DEL phenotype in Rh negative blood, thus preventing the anti-D immunization. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/28026/1/TD_NOR%20ARINA%20CHE%20MOHD%20NOOR%20HS%2015_5.pdf Che Mohd Noor, Nor Arina (2015) Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor. Degree thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Clinical pathology. Laboratory technique Examination of the blood
spellingShingle	Clinical pathology. Laboratory technique Examination of the blood Che Mohd Noor, Nor Arina Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor
description	DEL phenotype is known as the weakest D antigen in the Rh blood group system. Serologically, DEL phenotype commonly mistyped as true Rh negative. An anti-D immunization may occurred in Rh negative recipient who received blood from the DEL phenotype donor. The goals of the present study were to detect DEL phenotype in Rh negative blood by using chloroform elution technique and specific sequence primer-polymerase chain reaction (SSP-PCR). A total of 43 Rh negative blood donor's samples from National Blood Centre were examined for the Rh phenotyping and the presence of DEL phenotype by using chloroform elution technique and SSPPCR. The Rh negative phenotypes consisted of 79.07% dce, 13.95% dCce, 4.65% dcEe and 2.33% dCe. Chloroform elution technique failed to detect DEL phenotype, but only 2.33% (1/43) Rh negative blood donor's sample was being detected as DEL phenotype by using SSP-PCR. In conclusion, based on these findings, chloroform elution technique might not be an effective method in detection of DEL phenotype. Sensitivity and specificity of molecular typing method can overcome the limitation of serological method in the clinical laboratory. Hence, it is highly recommended to use SSP-PCR in detection of DEL phenotype in Rh negative blood, thus preventing the anti-D immunization.
format	Thesis
author	Che Mohd Noor, Nor Arina
author_facet	Che Mohd Noor, Nor Arina
author_sort	Che Mohd Noor, Nor Arina
title	Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor
title_short	Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor
title_full	Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor
title_fullStr	Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor
title_full_unstemmed	Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor
title_sort	detection of del phenotype in rh negative blood by using chloroform elution technique and specific sequence primer-polymerase chain reaction (ssp-pcr) / nor arina che mohd noor
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/28026/ http://ir.uitm.edu.my/id/eprint/28026/1/TD_NOR%20ARINA%20CHE%20MOHD%20NOOR%20HS%2015_5.pdf
first_indexed	2023-09-18T23:19:27Z
last_indexed	2023-09-18T23:19:27Z
_version_	1777419332702175232

Detection of Del Phenotype in RH negative blood by using chloroform elution technique and specific sequence Primer-Polymerase Chain Reaction (SSP-PCR) / Nor Arina Che Mohd Noor

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