Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz

Streptococcus agalactiae or Group B Streptococcus (GBS) is a causative agent for serious infection and neonatal sepsis that lead to neonatal morbidity and mortality. Previous study proved that the pathogenesis of Streptococcus agalactiae infection is complex and the severity can be predicted by the...

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Main Author:	Abdul Aziz, Muhammad Syafiq
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Clinical pathology. Laboratory technique Antibiotic therapy. Antibiotics
Online Access:	http://ir.uitm.edu.my/id/eprint/27991/ http://ir.uitm.edu.my/id/eprint/27991/1/TD_MUHAMMAD%20SYAFIQ%20ABDUL%20AZIZ%20HS%2015_5.pdf

id	uitm-27991
recordtype	eprints
spelling	uitm-279912020-02-06T05:09:50Z http://ir.uitm.edu.my/id/eprint/27991/ Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz Abdul Aziz, Muhammad Syafiq Clinical pathology. Laboratory technique Antibiotic therapy. Antibiotics Streptococcus agalactiae or Group B Streptococcus (GBS) is a causative agent for serious infection and neonatal sepsis that lead to neonatal morbidity and mortality. Previous study proved that the pathogenesis of Streptococcus agalactiae infection is complex and the severity can be predicted by the identification of the virulence factors encoded among others by the cps gene cluster coding of the Streptococcus agalactiae capsule. However, there is a lack of study done on the laboratory strains Streptococcus agalactiae. Study done stated that the laboratory strains are unable to fully represent microorganism that growth in the 'real-world' environment. This study is done to identify the virulence genes and antibiotic resistance genes in Streptococcus agalactiae isolates from laboratory reference strains. The laboratory strains Streptococcus agalactiae are sub-cultured into two different time frames in order to observe for any genetic drift or variations. The detection of the virulence gene 1mb, cylE, bca, scpB, and rib gene and antibiotic resistant genes ermB gene and tetO gene are done by using SYBR Green real-time PCR. All the samples from both short term and long term time frame were analyzed for the presence of the virulence and antibiotic resistant genes. The outcomes of the analysis indicate there were no genetic drifts or variants for 1mb, cylE bca, scpB gene even after a series of passage. The rib gene is undetermined for genetic variations or drift since not all the sample expressed the gene. The ermB gene and tetO gene analysis were undetermined since the results of the analysis are not specific. The comparison between clinical sample and laboratory strains show that the laboratory strains expressed the same virulence genes as the clinical samples. This study suggested that the used of real-time PCR are specific and sensitive methods that could be used to detect the virulence gene of Streptococcus agalactiae. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27991/1/TD_MUHAMMAD%20SYAFIQ%20ABDUL%20AZIZ%20HS%2015_5.pdf Abdul Aziz, Muhammad Syafiq (2015) Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz. Degree thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Clinical pathology. Laboratory technique Antibiotic therapy. Antibiotics
spellingShingle	Clinical pathology. Laboratory technique Antibiotic therapy. Antibiotics Abdul Aziz, Muhammad Syafiq Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz
description	Streptococcus agalactiae or Group B Streptococcus (GBS) is a causative agent for serious infection and neonatal sepsis that lead to neonatal morbidity and mortality. Previous study proved that the pathogenesis of Streptococcus agalactiae infection is complex and the severity can be predicted by the identification of the virulence factors encoded among others by the cps gene cluster coding of the Streptococcus agalactiae capsule. However, there is a lack of study done on the laboratory strains Streptococcus agalactiae. Study done stated that the laboratory strains are unable to fully represent microorganism that growth in the 'real-world' environment. This study is done to identify the virulence genes and antibiotic resistance genes in Streptococcus agalactiae isolates from laboratory reference strains. The laboratory strains Streptococcus agalactiae are sub-cultured into two different time frames in order to observe for any genetic drift or variations. The detection of the virulence gene 1mb, cylE, bca, scpB, and rib gene and antibiotic resistant genes ermB gene and tetO gene are done by using SYBR Green real-time PCR. All the samples from both short term and long term time frame were analyzed for the presence of the virulence and antibiotic resistant genes. The outcomes of the analysis indicate there were no genetic drifts or variants for 1mb, cylE bca, scpB gene even after a series of passage. The rib gene is undetermined for genetic variations or drift since not all the sample expressed the gene. The ermB gene and tetO gene analysis were undetermined since the results of the analysis are not specific. The comparison between clinical sample and laboratory strains show that the laboratory strains expressed the same virulence genes as the clinical samples. This study suggested that the used of real-time PCR are specific and sensitive methods that could be used to detect the virulence gene of Streptococcus agalactiae.
format	Thesis
author	Abdul Aziz, Muhammad Syafiq
author_facet	Abdul Aziz, Muhammad Syafiq
author_sort	Abdul Aziz, Muhammad Syafiq
title	Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz
title_short	Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz
title_full	Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz
title_fullStr	Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz
title_full_unstemmed	Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz
title_sort	identification of virulence and antibiotic resistant gene in laboratory strains streptococcus agalactiae / muhammad syafiq abdul aziz
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/27991/ http://ir.uitm.edu.my/id/eprint/27991/1/TD_MUHAMMAD%20SYAFIQ%20ABDUL%20AZIZ%20HS%2015_5.pdf
first_indexed	2023-09-18T23:19:22Z
last_indexed	2023-09-18T23:19:22Z
_version_	1777419328339050496

Identification of virulence and antibiotic resistant gene in laboratory strains Streptococcus agalactiae / Muhammad Syafiq Abdul Aziz

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