Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama

Immobilization of the enzymes on inorganic clay mineral is very useful in practical applications to overcome limitations of immobilized enzyme such as low long term stability and poor reusability of enzyme. Inorganic clay mineral proved to be an excellent supporting material for immobilization of en...

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Main Author:	Edama, Nurul Aini
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Plant physiology SB Plant culture
Online Access:	http://ir.uitm.edu.my/id/eprint/27930/ http://ir.uitm.edu.my/id/eprint/27930/1/TM_NURUL%20AINI%20EDAMA%20AT%2015_5.pdf

id	uitm-27930
recordtype	eprints
spelling	uitm-279302020-01-30T05:22:55Z http://ir.uitm.edu.my/id/eprint/27930/ Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama Edama, Nurul Aini Plant physiology SB Plant culture Immobilization of the enzymes on inorganic clay mineral is very useful in practical applications to overcome limitations of immobilized enzyme such as low long term stability and poor reusability of enzyme. Inorganic clay mineral proved to be an excellent supporting material for immobilization of enzymes by encapsulation technique. In this research, two types of clay material with different characteristics were evaluated for the enzyme immobilization process. The clay that used in this research was obtained from natural and palm oil processing waste clay sources. Characterization study has been done for both clay material using BET Surface Area, X-Ray Flouresence (XRF), Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM) analysis. The enzymes were encapsulated on the alg-clay beads where the alginate was used as a medium to be mixed with clay to encapsulate enzymes in the form of beads. The enzymes were successfully encapsulated within algclay beads with enzyme loading efficiency was more than 90%. The result also revealed that the highest immobilization yield was obtained at 2% (w/v) of the clay concentration for both types of clay material. From the reusability study, the results proved that immobilization of the enzymes onto the clay supporting material showed a high stability and availability for multiple uses as it could be reused up to seven cycles. The relative activity of enzyme immobilized on treated clay retained about 50% activity, meanwhile, the immobilized enzyme on untreated and waste clay retained 23% and 32% of activity respectively. Overall, the use of clay from natural and waste sources as enzymes supporting materials was successfully done in this research. Different sources and characteristic of clay could result in the different performance of tapioca saccharafication process. This method could have significant economic impact on the industrial of bioconversion of starch into glucose as it has low cost of the materials and its ability to retained the enzyme for many times and can be a good potential of enzymes supporting material in immobilization technology. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27930/1/TM_NURUL%20AINI%20EDAMA%20AT%2015_5.pdf Edama, Nurul Aini (2015) Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Plant physiology SB Plant culture
spellingShingle	Plant physiology SB Plant culture Edama, Nurul Aini Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama
description	Immobilization of the enzymes on inorganic clay mineral is very useful in practical applications to overcome limitations of immobilized enzyme such as low long term stability and poor reusability of enzyme. Inorganic clay mineral proved to be an excellent supporting material for immobilization of enzymes by encapsulation technique. In this research, two types of clay material with different characteristics were evaluated for the enzyme immobilization process. The clay that used in this research was obtained from natural and palm oil processing waste clay sources. Characterization study has been done for both clay material using BET Surface Area, X-Ray Flouresence (XRF), Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM) analysis. The enzymes were encapsulated on the alg-clay beads where the alginate was used as a medium to be mixed with clay to encapsulate enzymes in the form of beads. The enzymes were successfully encapsulated within algclay beads with enzyme loading efficiency was more than 90%. The result also revealed that the highest immobilization yield was obtained at 2% (w/v) of the clay concentration for both types of clay material. From the reusability study, the results proved that immobilization of the enzymes onto the clay supporting material showed a high stability and availability for multiple uses as it could be reused up to seven cycles. The relative activity of enzyme immobilized on treated clay retained about 50% activity, meanwhile, the immobilized enzyme on untreated and waste clay retained 23% and 32% of activity respectively. Overall, the use of clay from natural and waste sources as enzymes supporting materials was successfully done in this research. Different sources and characteristic of clay could result in the different performance of tapioca saccharafication process. This method could have significant economic impact on the industrial of bioconversion of starch into glucose as it has low cost of the materials and its ability to retained the enzyme for many times and can be a good potential of enzymes supporting material in immobilization technology.
format	Thesis
author	Edama, Nurul Aini
author_facet	Edama, Nurul Aini
author_sort	Edama, Nurul Aini
title	Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama
title_short	Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama
title_full	Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama
title_fullStr	Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama
title_full_unstemmed	Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama
title_sort	characterization and performance evaluation of enzymes supporting material developed from sg. sayong clay and waste clay for tapioca saccharification process / nurul aini edama
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/27930/ http://ir.uitm.edu.my/id/eprint/27930/1/TM_NURUL%20AINI%20EDAMA%20AT%2015_5.pdf
first_indexed	2023-09-18T23:19:16Z
last_indexed	2023-09-18T23:19:16Z
_version_	1777419321491849216

Characterization and performance evaluation of enzymes supporting material developed from Sg. Sayong clay and waste clay for tapioca saccharification process / Nurul Aini Edama

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