Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan

Engineered nanoparticles have been extensively explored in various biomedical settings including nanoparticulate imaging agents due to its promising benefits to mankind. Iodine-intolerance patients have caused alarming concerns in searching new contrast media with lower toxicity effect. However, pro...

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Main Author:	Ridwan, Rasdin
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Clinical pathology. Laboratory technique
Online Access:	http://ir.uitm.edu.my/id/eprint/27768/ http://ir.uitm.edu.my/id/eprint/27768/1/TD_RASDIN%20RIDWAN%20HS%2015_5.pdf

id	uitm-27768
recordtype	eprints
spelling	uitm-277682020-01-30T08:14:14Z http://ir.uitm.edu.my/id/eprint/27768/ Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan Ridwan, Rasdin Clinical pathology. Laboratory technique Engineered nanoparticles have been extensively explored in various biomedical settings including nanoparticulate imaging agents due to its promising benefits to mankind. Iodine-intolerance patients have caused alarming concerns in searching new contrast media with lower toxicity effect. However, proper potential mechanism of nanoparticles has yet to be fully established despite its early acceptance and emerging usage. By using animal model system, the aim of present study is to assess acute nanotoxicity of 14 nm iron oxide nanoparticles (IONPs) coated with nitric acid and silane-polyethylene glycol (SiPEG) in comparison to iodine. Eighteen male wistar rats were used in order to explore the underlying toxicity of IONPs in liver tissues. ·OH free radicals were elucidated by using reactive oxygen species (ROS) production assay and western blotting for the presence of p53 protein expression. The results revealed coated IONPs produced higher ROS level compared to iodine, however no statistical significant were observed. It can be hypothesized that higher amount of antioxidant has been produced by rats in iodine group in correlation to combat higher amount of ROS produced before assayed was conducted. Stronger expressions of p53 protein from the coated IONPs group were further observed. Expression of p53 protein suggested initiation of antioxidants production by the protein to ameliorate intracellular ROS production to further achieve normal redox balance in curtailing further damage. The outcomes highlighted that short-term administration of IONPs with different functionalization surfaces may be safe to be applied as radiographic contrast media. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27768/1/TD_RASDIN%20RIDWAN%20HS%2015_5.pdf Ridwan, Rasdin (2015) Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan. Degree thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Clinical pathology. Laboratory technique
spellingShingle	Clinical pathology. Laboratory technique Ridwan, Rasdin Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan
description	Engineered nanoparticles have been extensively explored in various biomedical settings including nanoparticulate imaging agents due to its promising benefits to mankind. Iodine-intolerance patients have caused alarming concerns in searching new contrast media with lower toxicity effect. However, proper potential mechanism of nanoparticles has yet to be fully established despite its early acceptance and emerging usage. By using animal model system, the aim of present study is to assess acute nanotoxicity of 14 nm iron oxide nanoparticles (IONPs) coated with nitric acid and silane-polyethylene glycol (SiPEG) in comparison to iodine. Eighteen male wistar rats were used in order to explore the underlying toxicity of IONPs in liver tissues. ·OH free radicals were elucidated by using reactive oxygen species (ROS) production assay and western blotting for the presence of p53 protein expression. The results revealed coated IONPs produced higher ROS level compared to iodine, however no statistical significant were observed. It can be hypothesized that higher amount of antioxidant has been produced by rats in iodine group in correlation to combat higher amount of ROS produced before assayed was conducted. Stronger expressions of p53 protein from the coated IONPs group were further observed. Expression of p53 protein suggested initiation of antioxidants production by the protein to ameliorate intracellular ROS production to further achieve normal redox balance in curtailing further damage. The outcomes highlighted that short-term administration of IONPs with different functionalization surfaces may be safe to be applied as radiographic contrast media.
format	Thesis
author	Ridwan, Rasdin
author_facet	Ridwan, Rasdin
author_sort	Ridwan, Rasdin
title	Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan
title_short	Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan
title_full	Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan
title_fullStr	Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan
title_full_unstemmed	Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan
title_sort	toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / rasdin ridwan
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/27768/ http://ir.uitm.edu.my/id/eprint/27768/1/TD_RASDIN%20RIDWAN%20HS%2015_5.pdf
first_indexed	2023-09-18T23:18:58Z
last_indexed	2023-09-18T23:18:58Z
_version_	1777419302848167936

Toxicity assessment of nitric acid functionalized with iron oxide nanoparticles and sipeg as a new radiographic contrast media / Rasdin Ridwan

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