Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan

Vegetables is an essential component of the healthy diet, which provides minerals, vitamins, and Phyto-nutrients. However, contaminated vegetables can be medium that spread diseases. The purpose of this study is to determine the coliform microbial loads of Lactuca sativa (lettuce) after being treat...

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Main Author:	Roslan, Muhammad Nabil
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2017
Subjects:	QR Microbiology Microbial ecology Bacteria
Online Access:	http://ir.uitm.edu.my/id/eprint/27477/ http://ir.uitm.edu.my/id/eprint/27477/1/PPb_MUHAMMAD%20NABIL%20ROSLAN%20AS%20N%2017_5.pdf

id	uitm-27477
recordtype	eprints
spelling	uitm-274772020-02-11T03:14:54Z http://ir.uitm.edu.my/id/eprint/27477/ Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan Roslan, Muhammad Nabil QR Microbiology Microbial ecology Bacteria Vegetables is an essential component of the healthy diet, which provides minerals, vitamins, and Phyto-nutrients. However, contaminated vegetables can be medium that spread diseases. The purpose of this study is to determine the coliform microbial loads of Lactuca sativa (lettuce) after being treat with different type of solutions namely turmeric extract, salt water and regular tap water. Lettuce sample was obtained from Kuala Pilah, Negeri Sembilan wet market. The procedure began with the treatment of lettuce sample with specified concentration of selected treatment solutions and were later enumerated on the MacConkey agar to count viable coliform colony. The Colony Forming Unit (CFU/g) was calculated and the result obtained shows a decrease of 70.45% for the turmeric solution when compared to standard Tap water treatment. CFU/g of lettuce sample treated with salt solution also shows a decrease of 69.83% when compared to the standard tap water treatment. Confirmation of coliform bacteria was conducted and several tests were performed for the suspected colonies on the plate. Among the tests done were the gram staining procedure to check the coliform's morphology. BGLB broth confirmation test for coliform and biochemical test (IMViC) to further identified the coliform species presence. The biochemical test gave a positive result for the presence of Escherichia coli. Faculty of Applied Sciences 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27477/1/PPb_MUHAMMAD%20NABIL%20ROSLAN%20AS%20N%2017_5.pdf Roslan, Muhammad Nabil (2017) Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology Microbial ecology Bacteria
spellingShingle	QR Microbiology Microbial ecology Bacteria Roslan, Muhammad Nabil Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan
description	Vegetables is an essential component of the healthy diet, which provides minerals, vitamins, and Phyto-nutrients. However, contaminated vegetables can be medium that spread diseases. The purpose of this study is to determine the coliform microbial loads of Lactuca sativa (lettuce) after being treat with different type of solutions namely turmeric extract, salt water and regular tap water. Lettuce sample was obtained from Kuala Pilah, Negeri Sembilan wet market. The procedure began with the treatment of lettuce sample with specified concentration of selected treatment solutions and were later enumerated on the MacConkey agar to count viable coliform colony. The Colony Forming Unit (CFU/g) was calculated and the result obtained shows a decrease of 70.45% for the turmeric solution when compared to standard Tap water treatment. CFU/g of lettuce sample treated with salt solution also shows a decrease of 69.83% when compared to the standard tap water treatment. Confirmation of coliform bacteria was conducted and several tests were performed for the suspected colonies on the plate. Among the tests done were the gram staining procedure to check the coliform's morphology. BGLB broth confirmation test for coliform and biochemical test (IMViC) to further identified the coliform species presence. The biochemical test gave a positive result for the presence of Escherichia coli.
format	Student Project
author	Roslan, Muhammad Nabil
author_facet	Roslan, Muhammad Nabil
author_sort	Roslan, Muhammad Nabil
title	Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan
title_short	Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan
title_full	Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan
title_fullStr	Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan
title_full_unstemmed	Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan
title_sort	enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / muhammad nabil roslan
publisher	Faculty of Applied Sciences
publishDate	2017
url	http://ir.uitm.edu.my/id/eprint/27477/ http://ir.uitm.edu.my/id/eprint/27477/1/PPb_MUHAMMAD%20NABIL%20ROSLAN%20AS%20N%2017_5.pdf
first_indexed	2023-09-18T23:18:29Z
last_indexed	2023-09-18T23:18:29Z
_version_	1777419271824998400

Enumeration of coliform bacteria from lactuca saliva (lettuce) sanitized with different type of sanitizers / Muhammad Nabil Roslan

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