In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi

A study was conducted to determine the effects of different plant regulators (PGR) towards in vitro regeneration and callus induction of new Malaysian rice variety of Oryza sativa L. var. MRIA 1. Intemode explants of 4-week old of aseptic seedlings were selected for in vitro regeneration and a total...

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Main Author:	Mohamad Fauzi, Nor Yasmin
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Plant anatomy Plant physiology Plant ecology
Online Access:	http://ir.uitm.edu.my/id/eprint/27186/ http://ir.uitm.edu.my/id/eprint/27186/1/TM_NOR%20YASMIN%20MOHAMAD%20FAUZI%20AS%2016_5.pdf

id	uitm-27186
recordtype	eprints
spelling	uitm-271862020-01-14T04:27:24Z http://ir.uitm.edu.my/id/eprint/27186/ In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi Mohamad Fauzi, Nor Yasmin Plant anatomy Plant physiology Plant ecology A study was conducted to determine the effects of different plant regulators (PGR) towards in vitro regeneration and callus induction of new Malaysian rice variety of Oryza sativa L. var. MRIA 1. Intemode explants of 4-week old of aseptic seedlings were selected for in vitro regeneration and a total of 16 types of different combinations of NAA, IBA, BAP, and Kn were utilised. The most suitable plant growth regulators (PGR) for regeneration of Oryza sativa L. var. MRIA 1 was obtained from MS (Murashige and Skoog) media supplemented with 1.0 mg/L NAA and 0.5 mg/L Kn with an average of 8.90 ± 1.79 number of shoots per explant, 114.00 ± 18.90 number of roots per explant, 10.70 ± 2.62 cm length of shoots per explant and 8.70 ± 1.64 number of leaves per explant. The effect of combination of different plant growth regulators (PGR) supplemented in culture media on dry weight of regenerated Oryza sativa L. var. MRIA 1 were determined. Highest fresh weight (4.35±1.57 g) and dry weight (0.44±0.11 g per explant) recorded on the MS media supplemented with 1.0 mg/L NAA and 0.5 mg/L Kn. The callus inductions were assessed from three different explants including root, internode, and leaf base. The findings showed that the addition of 1.0 mg/L BAP into MS media was the most suitable for callus induction from root explant since it yield the highest fresh weight (1.039 ± 0.935 g per explant) and dry weight (0.093 ± 0.065 g per explant). The findings provided and optimum plant growth regulators (PGR) concentration for the tissue culture sysytem of Oryza sativa L. var. MRIA 1. Acclimatization of regenerated Oryza sativa L. var. MRIA 1 is recommended in order to serve a complete in vitro regeneration protocol of Oryza sativa L. var. MRIA 1. 2016 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27186/1/TM_NOR%20YASMIN%20MOHAMAD%20FAUZI%20AS%2016_5.pdf Mohamad Fauzi, Nor Yasmin (2016) In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Plant anatomy Plant physiology Plant ecology
spellingShingle	Plant anatomy Plant physiology Plant ecology Mohamad Fauzi, Nor Yasmin In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
description	A study was conducted to determine the effects of different plant regulators (PGR) towards in vitro regeneration and callus induction of new Malaysian rice variety of Oryza sativa L. var. MRIA 1. Intemode explants of 4-week old of aseptic seedlings were selected for in vitro regeneration and a total of 16 types of different combinations of NAA, IBA, BAP, and Kn were utilised. The most suitable plant growth regulators (PGR) for regeneration of Oryza sativa L. var. MRIA 1 was obtained from MS (Murashige and Skoog) media supplemented with 1.0 mg/L NAA and 0.5 mg/L Kn with an average of 8.90 ± 1.79 number of shoots per explant, 114.00 ± 18.90 number of roots per explant, 10.70 ± 2.62 cm length of shoots per explant and 8.70 ± 1.64 number of leaves per explant. The effect of combination of different plant growth regulators (PGR) supplemented in culture media on dry weight of regenerated Oryza sativa L. var. MRIA 1 were determined. Highest fresh weight (4.35±1.57 g) and dry weight (0.44±0.11 g per explant) recorded on the MS media supplemented with 1.0 mg/L NAA and 0.5 mg/L Kn. The callus inductions were assessed from three different explants including root, internode, and leaf base. The findings showed that the addition of 1.0 mg/L BAP into MS media was the most suitable for callus induction from root explant since it yield the highest fresh weight (1.039 ± 0.935 g per explant) and dry weight (0.093 ± 0.065 g per explant). The findings provided and optimum plant growth regulators (PGR) concentration for the tissue culture sysytem of Oryza sativa L. var. MRIA 1. Acclimatization of regenerated Oryza sativa L. var. MRIA 1 is recommended in order to serve a complete in vitro regeneration protocol of Oryza sativa L. var. MRIA 1.
format	Thesis
author	Mohamad Fauzi, Nor Yasmin
author_facet	Mohamad Fauzi, Nor Yasmin
author_sort	Mohamad Fauzi, Nor Yasmin
title	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_short	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_full	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_fullStr	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_full_unstemmed	In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi
title_sort	in vitro regeneration and callus induction of oryza sativa l. var. mria 1 / nor yasmin mohamad fauzi
publishDate	2016
url	http://ir.uitm.edu.my/id/eprint/27186/ http://ir.uitm.edu.my/id/eprint/27186/1/TM_NOR%20YASMIN%20MOHAMAD%20FAUZI%20AS%2016_5.pdf
first_indexed	2023-09-18T23:17:57Z
last_indexed	2023-09-18T23:17:57Z
_version_	1777419238844137472

In vitro regeneration and callus induction of Oryza sativa L. Var. MRIA 1 / Nor Yasmin Mohamad Fauzi

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