Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar

Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar

Rennellia ellitpica Korth. is a tropical shrub that can be found in lowland tropical rainforest in Malaysia. It is locally known as 'segemuk' and dubbed as Malaysia Ginseng due to the resemblance between roots of R. elliptica and roots of ginseng, as well as its many traditional uses. The...

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Main Authors: Ismail, Nor Hadiani, Ahmad, Rohaya, Mohd Jaafar, Faridahanim
Format: Research Reports
Language:English
Published: Research Management Institute (RMI) 2011
Subjects:
Plant anatomy
Plant physiology
Online Access:http://ir.uitm.edu.my/id/eprint/27128/
http://ir.uitm.edu.my/id/eprint/27128/1/LP_NOR%20HADIANI%20ISMAIL%20RMI%2011_5.pdf
id uitm-27128
recordtype eprints
spelling uitm-271282020-01-09T05:55:42Z http://ir.uitm.edu.my/id/eprint/27128/ Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar Ismail, Nor Hadiani Ahmad, Rohaya Mohd Jaafar, Faridahanim Plant anatomy Plant physiology Rennellia ellitpica Korth. is a tropical shrub that can be found in lowland tropical rainforest in Malaysia. It is locally known as 'segemuk' and dubbed as Malaysia Ginseng due to the resemblance between roots of R. elliptica and roots of ginseng, as well as its many traditional uses. The decoction of the roots of this plant is traditionally used as treatment of body aches, as afterbirth tonic and also being claimed as aphrodisiac. The dried roots of R. elliptica that were previously collected from Taman Negara Kuala Keniam, Pahang were successively extracted using hexane, dichloromethane and methanol. The dichloromethane extract were absorbed onto acid washed silica (previously shaken with 4% oxalic acid, filtered and activated at 89 °C) and introduced to acid washed silica gel bed (60 cm X 5 cm) eluted with series of solvents, comprising of various compositions of hexanedichloromethane and dichloromethane-methanol in increasing polarity. Further isolation and purification utilizing various chromatographic procedures followed by careful analysis of spectral data including MS, IR, UV, 1D and 2D NMR as well as x-ray crystallography lead to identification of one new anthraquinone, 1,2-dimethoxy-6-methyl-9,10-anthraquinone (1) along with ten known anthraquinones namely nordamnacanthal (2), 2-formyl-3-hydroxy-9.10- anthraquinone (3), damnacanthal (4), 1-hydroxy-2-methoxy-6-methyl-9,10-anthraquinone (5), lucidin-cj-methyl ether (6), 3-hydroxy-2-methyl-9,10-anthraquinone (7), rubiadin (8), 2- hydroxy-3-methoxy-6-methyl-9,10-anthraquinone (9), rubiadin-1-methyl ether (10) and 3- hydroxy-2-hydroxymethyl-9,10-anthraquinone (11). The dichloromethane crude extract and the anthraquinones were screened for antiplasmodial activity in vitro. The dichloromethane extract inhibited the Plasmodium falciparum growth in vitro with IC50 value of 4.04 (jg/ml while most of anthraquinones tested were active inhibitors and the strongest inhibitor was shown by 3-hydroxy-2-methyl-9,10-anthraquinone with IC500.34|jM. The new anthraquinone, 1,2-dimethoxy-6-methyl-9,10-anthraquinone is an active inhibitor with IC50 value of 1.10 |jM. Research Management Institute (RMI) 2011-03 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27128/1/LP_NOR%20HADIANI%20ISMAIL%20RMI%2011_5.pdf Ismail, Nor Hadiani and Ahmad, Rohaya and Mohd Jaafar, Faridahanim (2011) Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar. [Research Reports] (Unpublished)
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Plant anatomy
Plant physiology
spellingShingle Plant anatomy
Plant physiology
Ismail, Nor Hadiani
Ahmad, Rohaya
Mohd Jaafar, Faridahanim
Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar
description Rennellia ellitpica Korth. is a tropical shrub that can be found in lowland tropical rainforest in Malaysia. It is locally known as 'segemuk' and dubbed as Malaysia Ginseng due to the resemblance between roots of R. elliptica and roots of ginseng, as well as its many traditional uses. The decoction of the roots of this plant is traditionally used as treatment of body aches, as afterbirth tonic and also being claimed as aphrodisiac. The dried roots of R. elliptica that were previously collected from Taman Negara Kuala Keniam, Pahang were successively extracted using hexane, dichloromethane and methanol. The dichloromethane extract were absorbed onto acid washed silica (previously shaken with 4% oxalic acid, filtered and activated at 89 °C) and introduced to acid washed silica gel bed (60 cm X 5 cm) eluted with series of solvents, comprising of various compositions of hexanedichloromethane and dichloromethane-methanol in increasing polarity. Further isolation and purification utilizing various chromatographic procedures followed by careful analysis of spectral data including MS, IR, UV, 1D and 2D NMR as well as x-ray crystallography lead to identification of one new anthraquinone, 1,2-dimethoxy-6-methyl-9,10-anthraquinone (1) along with ten known anthraquinones namely nordamnacanthal (2), 2-formyl-3-hydroxy-9.10- anthraquinone (3), damnacanthal (4), 1-hydroxy-2-methoxy-6-methyl-9,10-anthraquinone (5), lucidin-cj-methyl ether (6), 3-hydroxy-2-methyl-9,10-anthraquinone (7), rubiadin (8), 2- hydroxy-3-methoxy-6-methyl-9,10-anthraquinone (9), rubiadin-1-methyl ether (10) and 3- hydroxy-2-hydroxymethyl-9,10-anthraquinone (11). The dichloromethane crude extract and the anthraquinones were screened for antiplasmodial activity in vitro. The dichloromethane extract inhibited the Plasmodium falciparum growth in vitro with IC50 value of 4.04 (jg/ml while most of anthraquinones tested were active inhibitors and the strongest inhibitor was shown by 3-hydroxy-2-methyl-9,10-anthraquinone with IC500.34|jM. The new anthraquinone, 1,2-dimethoxy-6-methyl-9,10-anthraquinone is an active inhibitor with IC50 value of 1.10 |jM.
format Research Reports
author Ismail, Nor Hadiani
Ahmad, Rohaya
Mohd Jaafar, Faridahanim
author_facet Ismail, Nor Hadiani
Ahmad, Rohaya
Mohd Jaafar, Faridahanim
author_sort Ismail, Nor Hadiani
title Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar
title_short Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar
title_full Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar
title_fullStr Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar
title_full_unstemmed Chemical constituents of Rennelua Elliptica: The Malaysian ginseng / Nor Hadiani Ismail, Rohaya Ahmad and Faridahanim Mohd Jaafar
title_sort chemical constituents of rennelua elliptica: the malaysian ginseng / nor hadiani ismail, rohaya ahmad and faridahanim mohd jaafar
publisher Research Management Institute (RMI)
publishDate 2011
url http://ir.uitm.edu.my/id/eprint/27128/
http://ir.uitm.edu.my/id/eprint/27128/1/LP_NOR%20HADIANI%20ISMAIL%20RMI%2011_5.pdf
first_indexed 2023-09-18T23:17:50Z
last_indexed 2023-09-18T23:17:50Z
_version_ 1777419231215747072

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