$The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai$

The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai

Background: Psoriasis is a chronic, immune-mediated, inflammatory disorder involving mainly the skin and joints. It is a global health problem which results in localised or extensive development of erythematous scaly plaques, interfering with daily activities. Psoriasis has no cure however available...

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Bibliographic Details
Main Author:	Mohd Azraai, Awla
Format:	Student Project
Language:	English
Published:	Faculty of Law 2017
Subjects:	Skin manifestations of systemic disease RM Therapeutics. Pharmacology Antibiotic therapy. Antibiotics
Online Access:	http://ir.uitm.edu.my/id/eprint/27067/ http://ir.uitm.edu.my/id/eprint/27067/1/TM_AWLA%20MOHD%20AZRAAI%20MD%2017_5.pdf

id	uitm-27067
recordtype	eprints
spelling	uitm-270672020-01-14T04:24:03Z http://ir.uitm.edu.my/id/eprint/27067/ The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai Mohd Azraai, Awla Skin manifestations of systemic disease RM Therapeutics. Pharmacology Antibiotic therapy. Antibiotics Background: Psoriasis is a chronic, immune-mediated, inflammatory disorder involving mainly the skin and joints. It is a global health problem which results in localised or extensive development of erythematous scaly plaques, interfering with daily activities. Psoriasis has no cure however available treatment aims at controlling symptoms despite having many side effects. Imiquimod (IMQ)-induced psoriasis murine model has been widely used in research as it mimics human psoriatic lesions at morphological, histological, immunological and genetic levels. Studies comparing the effects of IMQ on male and female mice are scarce. Tocotrienols have anti-oxidative, anti-inflammatory, anti-mitotic and anti-angiogenic properties making them a potential therapeutic candidate for psoriasis. Aim: 1) To compare between IMQinduced psoriatic lesions in male and female mice. 2) To investigate the clinical and histological effects of TRF application on the IMQ-induced psoriatic lesions. Method: Twenty-three 8-12 weeks old BALB/c mice were distributed into 4 groups; a female IMQ-TRF, male IMQ-TRF, IMQ-Placebo and IMQ control group. IMQ was applied topically to shaved backs of mice for six days. Psoriasis severity was scored every two days, using the Psoriasis Area Severity Index (PASI) including erythema, thickness and scaling. Tocotrienol-rich fraction (TRF) was applied on the skin lesions for 10 days and the PASI score recorded. The mice were sacrificed and back skin excised then examined histologically. Results: Both male and female mice developed similar clinical lesions following IMQ induction and the histological changes were comparable with features of human psoriasis. TRF improved the psoriatic lesions in male and female mice in similar days. Placebo treatment resulted in significantly less healing time compared to TRF. The histological analysis of the mice skin following TRF and placebo treatment showed little difference. Conclusion: Both male and female IMQ psoriasis mice model may be used. TRF ameliorates psoriatic lesions in mice however placebo resulted in less healing time. Red palm oil has potential therapeutic effects on psoriatic lesions. Faculty of Law 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27067/1/TM_AWLA%20MOHD%20AZRAAI%20MD%2017_5.pdf Mohd Azraai, Awla (2017) The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Skin manifestations of systemic disease RM Therapeutics. Pharmacology Antibiotic therapy. Antibiotics
spellingShingle	Skin manifestations of systemic disease RM Therapeutics. Pharmacology Antibiotic therapy. Antibiotics Mohd Azraai, Awla The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai
description	Background: Psoriasis is a chronic, immune-mediated, inflammatory disorder involving mainly the skin and joints. It is a global health problem which results in localised or extensive development of erythematous scaly plaques, interfering with daily activities. Psoriasis has no cure however available treatment aims at controlling symptoms despite having many side effects. Imiquimod (IMQ)-induced psoriasis murine model has been widely used in research as it mimics human psoriatic lesions at morphological, histological, immunological and genetic levels. Studies comparing the effects of IMQ on male and female mice are scarce. Tocotrienols have anti-oxidative, anti-inflammatory, anti-mitotic and anti-angiogenic properties making them a potential therapeutic candidate for psoriasis. Aim: 1) To compare between IMQinduced psoriatic lesions in male and female mice. 2) To investigate the clinical and histological effects of TRF application on the IMQ-induced psoriatic lesions. Method: Twenty-three 8-12 weeks old BALB/c mice were distributed into 4 groups; a female IMQ-TRF, male IMQ-TRF, IMQ-Placebo and IMQ control group. IMQ was applied topically to shaved backs of mice for six days. Psoriasis severity was scored every two days, using the Psoriasis Area Severity Index (PASI) including erythema, thickness and scaling. Tocotrienol-rich fraction (TRF) was applied on the skin lesions for 10 days and the PASI score recorded. The mice were sacrificed and back skin excised then examined histologically. Results: Both male and female mice developed similar clinical lesions following IMQ induction and the histological changes were comparable with features of human psoriasis. TRF improved the psoriatic lesions in male and female mice in similar days. Placebo treatment resulted in significantly less healing time compared to TRF. The histological analysis of the mice skin following TRF and placebo treatment showed little difference. Conclusion: Both male and female IMQ psoriasis mice model may be used. TRF ameliorates psoriatic lesions in mice however placebo resulted in less healing time. Red palm oil has potential therapeutic effects on psoriatic lesions.
format	Student Project
author	Mohd Azraai, Awla
author_facet	Mohd Azraai, Awla
author_sort	Mohd Azraai, Awla
title	The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai
title_short	The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai
title_full	The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai
title_fullStr	The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai
title_full_unstemmed	The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai
title_sort	effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / awla mohd azraai
publisher	Faculty of Law
publishDate	2017
url	http://ir.uitm.edu.my/id/eprint/27067/ http://ir.uitm.edu.my/id/eprint/27067/1/TM_AWLA%20MOHD%20AZRAAI%20MD%2017_5.pdf
first_indexed	2023-09-18T23:17:42Z
last_indexed	2023-09-18T23:17:42Z
_version_	1777419222801973248

The effects of tocotrienolrich fraction on imiquimodinduced psoriatic lesion in mice / Awla Mohd Azraai

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