Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]

Metformin has anticancer effects by inhibiting glucose metabolism. Hyperthermia sensitizes cancer cells. It is known that both metformin,hypo- and hyperthermia deregulate mitochondrial metabolism in cancer. However, the mechanism of the additive effect is not known. The combined effect of metformin...

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Main Authors:	Mahmood Al- Khateeb, Alyaa R, Froemming, Gabriele Ruth Anisah, Mohd Ismail, Aletza, Abdul Hamid Hasani, Narimah
Format:	Research Reports
Language:	English
Published:	Research Management Institute (RMI) 2013
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://ir.uitm.edu.my/id/eprint/26751/ http://ir.uitm.edu.my/id/eprint/26751/1/LP_ALYAA%20R%20MAHMOOD%20AL-%20KHATEEB%20RMI%2013_5.pdf

id	uitm-26751
recordtype	eprints
spelling	uitm-267512019-12-09T04:08:18Z http://ir.uitm.edu.my/id/eprint/26751/ Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.] Mahmood Al- Khateeb, Alyaa R Froemming, Gabriele Ruth Anisah Mohd Ismail, Aletza Abdul Hamid Hasani, Narimah RC0254 Neoplasms. Tumors. Oncology (including Cancer) Metformin has anticancer effects by inhibiting glucose metabolism. Hyperthermia sensitizes cancer cells. It is known that both metformin,hypo- and hyperthermia deregulate mitochondrial metabolism in cancer. However, the mechanism of the additive effect is not known. The combined effect of metformin and hypo- or hyperthermia on osteosarcoma cell line (MG-63 cell) viability, DNA damage, glucose metabolism, regulation of apoptotic genes and proteins were investigated. Osteosarcoma (MG-63) cells were treated with metformin IC50 30M for 48h followed by exposure to 45°C, 39°C, 35°C 27°C for 30 min, 1h and 2h , 37°C was served as control. The combinations of metformin with hypo- and hyperthermia significantly downregulated the expression of AKT1 and GSK3(3 genes, which led to a reduce cells viability and an increase in DNA damage. Metformin induced apoptosis and necrosis. The combined effect caused a downregulation of anti-apoptotic Bcl-2. The intrinsic pathway was not activated. Therefore, this study suggests that the combination of metformin with hyperthermia particularly 45°C, enhances apoptosis via Bax/Bid-dependent pathway. Research Management Institute (RMI) 2013 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/26751/1/LP_ALYAA%20R%20MAHMOOD%20AL-%20KHATEEB%20RMI%2013_5.pdf Mahmood Al- Khateeb, Alyaa R and Froemming, Gabriele Ruth Anisah and Mohd Ismail, Aletza and Abdul Hamid Hasani, Narimah (2013) Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]. [Research Reports] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mahmood Al- Khateeb, Alyaa R Froemming, Gabriele Ruth Anisah Mohd Ismail, Aletza Abdul Hamid Hasani, Narimah Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]
description	Metformin has anticancer effects by inhibiting glucose metabolism. Hyperthermia sensitizes cancer cells. It is known that both metformin,hypo- and hyperthermia deregulate mitochondrial metabolism in cancer. However, the mechanism of the additive effect is not known. The combined effect of metformin and hypo- or hyperthermia on osteosarcoma cell line (MG-63 cell) viability, DNA damage, glucose metabolism, regulation of apoptotic genes and proteins were investigated. Osteosarcoma (MG-63) cells were treated with metformin IC50 30M for 48h followed by exposure to 45°C, 39°C, 35°C 27°C for 30 min, 1h and 2h , 37°C was served as control. The combinations of metformin with hypo- and hyperthermia significantly downregulated the expression of AKT1 and GSK3(3 genes, which led to a reduce cells viability and an increase in DNA damage. Metformin induced apoptosis and necrosis. The combined effect caused a downregulation of anti-apoptotic Bcl-2. The intrinsic pathway was not activated. Therefore, this study suggests that the combination of metformin with hyperthermia particularly 45°C, enhances apoptosis via Bax/Bid-dependent pathway.
format	Research Reports
author	Mahmood Al- Khateeb, Alyaa R Froemming, Gabriele Ruth Anisah Mohd Ismail, Aletza Abdul Hamid Hasani, Narimah
author_facet	Mahmood Al- Khateeb, Alyaa R Froemming, Gabriele Ruth Anisah Mohd Ismail, Aletza Abdul Hamid Hasani, Narimah
author_sort	Mahmood Al- Khateeb, Alyaa R
title	Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]
title_short	Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]
title_full	Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]
title_fullStr	Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]
title_full_unstemmed	Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]
title_sort	targeting heat shock response in osteosarcoma cells by combination treatment / dr. alyaa r mahmood al- khateeb … [et al.]
publisher	Research Management Institute (RMI)
publishDate	2013
url	http://ir.uitm.edu.my/id/eprint/26751/ http://ir.uitm.edu.my/id/eprint/26751/1/LP_ALYAA%20R%20MAHMOOD%20AL-%20KHATEEB%20RMI%2013_5.pdf
first_indexed	2023-09-18T23:17:24Z
last_indexed	2023-09-18T23:17:24Z
_version_	1777419204295655424

Targeting heat shock response in osteosarcoma cells by combination treatment / Dr. Alyaa R Mahmood Al- Khateeb … [et al.]

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