Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam

Parkia speciosa seeds were used in this research to investigate Thin Layer Chromatography (TLC) profile and its ability for antibacterial activity as well as to identify the secondary metabolites present through phytochemical screening. The seeds undergo cold extraction process after grinding into p...

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Main Author:	Akryl Azam, Nur Syahida
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2017
Subjects:	Extraction (Chemistry) Plant ecology
Online Access:	http://ir.uitm.edu.my/id/eprint/26271/ http://ir.uitm.edu.my/id/eprint/26271/1/PPb_NUR%20SYAHIDA%20AKRYL%20AZAM%20AS%20N%2017_5.pdf

id	uitm-26271
recordtype	eprints
spelling	uitm-262712019-10-22T04:55:32Z http://ir.uitm.edu.my/id/eprint/26271/ Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam Akryl Azam, Nur Syahida Extraction (Chemistry) Plant ecology Parkia speciosa seeds were used in this research to investigate Thin Layer Chromatography (TLC) profile and its ability for antibacterial activity as well as to identify the secondary metabolites present through phytochemical screening. The seeds undergo cold extraction process after grinding into powder. The powdered seeds were macerated successively into hexane, ethyl acetate and methanol for 72 hours at room temperature and were concentrated by rotary evaporator to obtain the crude extracts. Each crude extracts were tested by using a suitable solvent polarity ratio to obtain the best respective TLC profile before proceed to phytochemical screening tests to confirmed the presence of flavonoid, alkaloid, terpenoid, phytosterol, steroid, phenol, tannin, saponin and glycoside. Ratio of hexane : ethyl acetate (2 : 3) eluent was the best to fractionate methanol crude extract meanwhile for ethyl acetate crude extract, hexane : acetone (3 : 2) was the most suitable eluent. For hexane crude extract, hexane : chloroform (1 : 4) was identified. Methanol crude extract showed positive results for the presence of flavonoid, alkaloid, terpenoid, steroid and glycoside. Whereas, tannin, phenol, steroid and terpenoid were present in ethyl acetate crude extracts. Only phytosterol was present in hexane crude extract. Two types of bacteria, Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram negative (Salmonella typhi and Escherichia coli) were used in the antibacterial activity using disc diffusion method. Streptomycin and dimethyl sulfoxide (DMSO) involved as the positive and negative control respectively. Only methanol crude extract of P. speciosa seeds showed the highest inhibition zone against B. subtilis with 11.5 mm while the lowest inhibition zone was against S. typhi with 7.5 mm for its antibacterial activity. Faculty of Applied Sciences 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/26271/1/PPb_NUR%20SYAHIDA%20AKRYL%20AZAM%20AS%20N%2017_5.pdf Akryl Azam, Nur Syahida (2017) Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Extraction (Chemistry) Plant ecology
spellingShingle	Extraction (Chemistry) Plant ecology Akryl Azam, Nur Syahida Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam
description	Parkia speciosa seeds were used in this research to investigate Thin Layer Chromatography (TLC) profile and its ability for antibacterial activity as well as to identify the secondary metabolites present through phytochemical screening. The seeds undergo cold extraction process after grinding into powder. The powdered seeds were macerated successively into hexane, ethyl acetate and methanol for 72 hours at room temperature and were concentrated by rotary evaporator to obtain the crude extracts. Each crude extracts were tested by using a suitable solvent polarity ratio to obtain the best respective TLC profile before proceed to phytochemical screening tests to confirmed the presence of flavonoid, alkaloid, terpenoid, phytosterol, steroid, phenol, tannin, saponin and glycoside. Ratio of hexane : ethyl acetate (2 : 3) eluent was the best to fractionate methanol crude extract meanwhile for ethyl acetate crude extract, hexane : acetone (3 : 2) was the most suitable eluent. For hexane crude extract, hexane : chloroform (1 : 4) was identified. Methanol crude extract showed positive results for the presence of flavonoid, alkaloid, terpenoid, steroid and glycoside. Whereas, tannin, phenol, steroid and terpenoid were present in ethyl acetate crude extracts. Only phytosterol was present in hexane crude extract. Two types of bacteria, Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram negative (Salmonella typhi and Escherichia coli) were used in the antibacterial activity using disc diffusion method. Streptomycin and dimethyl sulfoxide (DMSO) involved as the positive and negative control respectively. Only methanol crude extract of P. speciosa seeds showed the highest inhibition zone against B. subtilis with 11.5 mm while the lowest inhibition zone was against S. typhi with 7.5 mm for its antibacterial activity.
format	Student Project
author	Akryl Azam, Nur Syahida
author_facet	Akryl Azam, Nur Syahida
author_sort	Akryl Azam, Nur Syahida
title	Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam
title_short	Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam
title_full	Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam
title_fullStr	Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam
title_full_unstemmed	Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam
title_sort	phytochemical screening of parkia speciosa seeds / nur syahida akryl azam
publisher	Faculty of Applied Sciences
publishDate	2017
url	http://ir.uitm.edu.my/id/eprint/26271/ http://ir.uitm.edu.my/id/eprint/26271/1/PPb_NUR%20SYAHIDA%20AKRYL%20AZAM%20AS%20N%2017_5.pdf
first_indexed	2023-09-18T23:16:33Z
last_indexed	2023-09-18T23:16:33Z
_version_	1777419150386266112

Phytochemical screening of parkia speciosa seeds / Nur Syahida Akryl Azam

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