A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman

A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman

The fact that the pigment of cosmetic contact lenses have a direct in contact to the cornea is worrisome. Up-to-date there was no single evaluation that proven the pigment in cosmetic contact lenses caused non-toxic to the eye. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide test (MTT...

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Bibliographic Details
Main Author: Abdul Rahman, Nur Fatihah
Format: Student Project
Language:English
Published: Faculty of Health Sciences 2015
Subjects:
RE Ophthalmology
RL Dermatology
Online Access:http://ir.uitm.edu.my/id/eprint/25988/
http://ir.uitm.edu.my/id/eprint/25988/1/PPb_NUR%20FATIHAH%20ABDUL%20RAHMAN%20HS%2015_5.pdf
id uitm-25988
recordtype eprints
spelling uitm-259882019-10-04T08:29:17Z http://ir.uitm.edu.my/id/eprint/25988/ A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman Abdul Rahman, Nur Fatihah RE Ophthalmology RL Dermatology The fact that the pigment of cosmetic contact lenses have a direct in contact to the cornea is worrisome. Up-to-date there was no single evaluation that proven the pigment in cosmetic contact lenses caused non-toxic to the eye. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide test (MTT) had been widely used as an alternative in vitro toxicity assay. The objectives of this was to systematically review method of toxicity test using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide test (MTT) and to analyze effectiveness of 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide test (MTT) tested on pigment of cosmetic contact lens. The review was done by an exhaustive search on health science databases and internet searching using the keywords related. The studies were then screened for their inclusion and exclusion criteria before extracted and analysis was done. The methodology done resulted in 27 studies selected and being tabulated. According to the review, immortalized human corneal epithelium was the most appropriate cell culture to be used. Both of the solid and liquid physical state of tested substance can be exposed to the culture cell. MTT assay method involved addition, incubation and extraction before colorimetrically measured using spectrophotometer. As a conclusion MTT method is an effective method in determining the toxicity of the pigment in cosmetic contact lens. Faculty of Health Sciences 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/25988/1/PPb_NUR%20FATIHAH%20ABDUL%20RAHMAN%20HS%2015_5.pdf Abdul Rahman, Nur Fatihah (2015) A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman. [Student Project] (Unpublished)
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic RE Ophthalmology
RL Dermatology
spellingShingle RE Ophthalmology
RL Dermatology
Abdul Rahman, Nur Fatihah
A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman
description The fact that the pigment of cosmetic contact lenses have a direct in contact to the cornea is worrisome. Up-to-date there was no single evaluation that proven the pigment in cosmetic contact lenses caused non-toxic to the eye. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide test (MTT) had been widely used as an alternative in vitro toxicity assay. The objectives of this was to systematically review method of toxicity test using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide test (MTT) and to analyze effectiveness of 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide test (MTT) tested on pigment of cosmetic contact lens. The review was done by an exhaustive search on health science databases and internet searching using the keywords related. The studies were then screened for their inclusion and exclusion criteria before extracted and analysis was done. The methodology done resulted in 27 studies selected and being tabulated. According to the review, immortalized human corneal epithelium was the most appropriate cell culture to be used. Both of the solid and liquid physical state of tested substance can be exposed to the culture cell. MTT assay method involved addition, incubation and extraction before colorimetrically measured using spectrophotometer. As a conclusion MTT method is an effective method in determining the toxicity of the pigment in cosmetic contact lens.
format Student Project
author Abdul Rahman, Nur Fatihah
author_facet Abdul Rahman, Nur Fatihah
author_sort Abdul Rahman, Nur Fatihah
title A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman
title_short A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman
title_full A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman
title_fullStr A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman
title_full_unstemmed A review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (MTT) / Nur Fatihah Abdul Rahman
title_sort review of pigment toxicity on cosmetic contact lens using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyl-tetrazolium bromide test (mtt) / nur fatihah abdul rahman
publisher Faculty of Health Sciences
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/25988/
http://ir.uitm.edu.my/id/eprint/25988/1/PPb_NUR%20FATIHAH%20ABDUL%20RAHMAN%20HS%2015_5.pdf
first_indexed 2023-09-18T23:15:55Z
last_indexed 2023-09-18T23:15:55Z
_version_ 1777419110724927488

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