Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman

Medicinal plants have many traditional claims including the treatment of ailments of infectious origin. In the evaluation of traditional claims, scientific research is important. The objective of the study was to evaluate the antibacterial activity of methanol extract of Licuala spinosa stems and to...

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Main Author:	Othman, Nurul Ariffah
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2014
Subjects:	Extraction (Chemistry) Microorganisms QR Microbiology
Online Access:	http://ir.uitm.edu.my/id/eprint/24873/ http://ir.uitm.edu.my/id/eprint/24873/1/PPb_NURUL%20ARIFFAH%20OTHMAN%20AS%20N%2014_5.pdf

id	uitm-24873
recordtype	eprints
spelling	uitm-248732019-07-25T04:13:23Z http://ir.uitm.edu.my/id/eprint/24873/ Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman Othman, Nurul Ariffah Extraction (Chemistry) Microorganisms QR Microbiology Medicinal plants have many traditional claims including the treatment of ailments of infectious origin. In the evaluation of traditional claims, scientific research is important. The objective of the study was to evaluate the antibacterial activity of methanol extract of Licuala spinosa stems and to evaluate the acute toxicity of methanol extract of Licuala spinosa stems. In this preliminary investigation, the extract was prepared through maceration of dried powdered stems of the plant by methanolic solution. The antibacterial activity of the crude methanol extracts was evaluated at eight different concentrations and subjected to screening against four strains of bacteria species, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Micrococcus luteus, using standard protocol of disc diffusion method. The methanol extracts was more active against Gram-positive bacteria as compared to Gram-negative bacteria. The antibacterial activities were assessed by the presence or absence of inhibition zones. The inhibitory effect of the extract was compared with standard antibiotic, vancomycin (positive control). The methanol extract of Licuala spinosa stems do not demonstrate any significant antibacterial activity compared with the negative control (p > 0.05). The plant extracts tested showed no antibacterial activities against all four bacterial that were used. Further study of this plant extracts has been tested for acute toxicity in Artemia salina. Methano 1 extracts from Licuala spinosa stems was screened by the brine shrimp lethality assay and found that the extract showed significant toxicity to the brine shrimp. Licuala spinosa stems extract have exhibited potent activity with LC50 38.9 μg/ml. Faculty of Applied Sciences 2014 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24873/1/PPb_NURUL%20ARIFFAH%20OTHMAN%20AS%20N%2014_5.pdf Othman, Nurul Ariffah (2014) Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Extraction (Chemistry) Microorganisms QR Microbiology
spellingShingle	Extraction (Chemistry) Microorganisms QR Microbiology Othman, Nurul Ariffah Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman
description	Medicinal plants have many traditional claims including the treatment of ailments of infectious origin. In the evaluation of traditional claims, scientific research is important. The objective of the study was to evaluate the antibacterial activity of methanol extract of Licuala spinosa stems and to evaluate the acute toxicity of methanol extract of Licuala spinosa stems. In this preliminary investigation, the extract was prepared through maceration of dried powdered stems of the plant by methanolic solution. The antibacterial activity of the crude methanol extracts was evaluated at eight different concentrations and subjected to screening against four strains of bacteria species, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Micrococcus luteus, using standard protocol of disc diffusion method. The methanol extracts was more active against Gram-positive bacteria as compared to Gram-negative bacteria. The antibacterial activities were assessed by the presence or absence of inhibition zones. The inhibitory effect of the extract was compared with standard antibiotic, vancomycin (positive control). The methanol extract of Licuala spinosa stems do not demonstrate any significant antibacterial activity compared with the negative control (p > 0.05). The plant extracts tested showed no antibacterial activities against all four bacterial that were used. Further study of this plant extracts has been tested for acute toxicity in Artemia salina. Methano 1 extracts from Licuala spinosa stems was screened by the brine shrimp lethality assay and found that the extract showed significant toxicity to the brine shrimp. Licuala spinosa stems extract have exhibited potent activity with LC50 38.9 μg/ml.
format	Student Project
author	Othman, Nurul Ariffah
author_facet	Othman, Nurul Ariffah
author_sort	Othman, Nurul Ariffah
title	Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman
title_short	Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman
title_full	Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman
title_fullStr	Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman
title_full_unstemmed	Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman
title_sort	evaluation of antibacterial activity and toxicity of licuala spinosa stems / nurul ariffah othman
publisher	Faculty of Applied Sciences
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/24873/ http://ir.uitm.edu.my/id/eprint/24873/1/PPb_NURUL%20ARIFFAH%20OTHMAN%20AS%20N%2014_5.pdf
first_indexed	2023-09-18T23:13:33Z
last_indexed	2023-09-18T23:13:33Z
_version_	1777418961912070144

Evaluation of antibacterial activity and toxicity of licuala spinosa stems / Nurul Ariffah Othman

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