Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin

People nowadays have very little consideration on food safety and practice poor eating habits such as eating raw or undercooked seafood. There is a presence of microorganism that is said to be opportunistic pathogens such as in cockles, there is a presence of Vibrio spp. which can cause vibriosis. T...

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Bibliographic Details
Main Author:	Kamaruddin, Nur Hajaratul Aswat
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2014
Subjects:	Microorganisms QR Microbiology
Online Access:	http://ir.uitm.edu.my/id/eprint/24742/ http://ir.uitm.edu.my/id/eprint/24742/1/PPb_NUR%20HAJARATUL%20ASWAT%20KAMARUDDIN%20AS%20N%2014_5.pdf

id	uitm-24742
recordtype	eprints
spelling	uitm-247422019-07-19T00:45:14Z http://ir.uitm.edu.my/id/eprint/24742/ Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin Kamaruddin, Nur Hajaratul Aswat Microorganisms QR Microbiology People nowadays have very little consideration on food safety and practice poor eating habits such as eating raw or undercooked seafood. There is a presence of microorganism that is said to be opportunistic pathogens such as in cockles, there is a presence of Vibrio spp. which can cause vibriosis. This study is to detect the presence of V spp. and V cholerae in cockles at three different retails in Kuala Pilah, Negeri Sembilan and the antibiotic susceptibility of that species. The findings of the study show that there is more than 2.4 x I 0 10 MPN/g in IO g of cockles from all three places. The result obtained by using Most Probable Number (MPN) method. Then in specifically determining of V cholerae, night market cockles were found to have highest rate of contamination with 71.43%. In order to clarify the presence of V spp. and V cholerae in cockles, Polymerase Chain Reaction (PCR) and visualization by Gel Electrophoresis had been done, proving that the presence of both species were positive. Lastly, for antibiotic test, the Multiple Antibiotic Resistance (MAR) and Disk Diffusion method were used and seven out of 14 types of antibiotics were found to be resistance since the MAR index was between 0.2 to 0.5. Bacitracin, Vancomycin, Furazididone, Tetracycline and Erythromycin are highly resistance to Vibrio spp. where Bacitracin is the best antibiotic to treat that species. Faculty of Applied Sciences 2014 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24742/1/PPb_NUR%20HAJARATUL%20ASWAT%20KAMARUDDIN%20AS%20N%2014_5.pdf Kamaruddin, Nur Hajaratul Aswat (2014) Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Microorganisms QR Microbiology
spellingShingle	Microorganisms QR Microbiology Kamaruddin, Nur Hajaratul Aswat Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
description	People nowadays have very little consideration on food safety and practice poor eating habits such as eating raw or undercooked seafood. There is a presence of microorganism that is said to be opportunistic pathogens such as in cockles, there is a presence of Vibrio spp. which can cause vibriosis. This study is to detect the presence of V spp. and V cholerae in cockles at three different retails in Kuala Pilah, Negeri Sembilan and the antibiotic susceptibility of that species. The findings of the study show that there is more than 2.4 x I 0 10 MPN/g in IO g of cockles from all three places. The result obtained by using Most Probable Number (MPN) method. Then in specifically determining of V cholerae, night market cockles were found to have highest rate of contamination with 71.43%. In order to clarify the presence of V spp. and V cholerae in cockles, Polymerase Chain Reaction (PCR) and visualization by Gel Electrophoresis had been done, proving that the presence of both species were positive. Lastly, for antibiotic test, the Multiple Antibiotic Resistance (MAR) and Disk Diffusion method were used and seven out of 14 types of antibiotics were found to be resistance since the MAR index was between 0.2 to 0.5. Bacitracin, Vancomycin, Furazididone, Tetracycline and Erythromycin are highly resistance to Vibrio spp. where Bacitracin is the best antibiotic to treat that species.
format	Student Project
author	Kamaruddin, Nur Hajaratul Aswat
author_facet	Kamaruddin, Nur Hajaratul Aswat
author_sort	Kamaruddin, Nur Hajaratul Aswat
title	Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_short	Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_full	Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_fullStr	Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_full_unstemmed	Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_sort	prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in kuala pilah, negeri sembilan. / nur hajaratul aswat kamaruddin
publisher	Faculty of Applied Sciences
publishDate	2014
url	http://ir.uitm.edu.my/id/eprint/24742/ http://ir.uitm.edu.my/id/eprint/24742/1/PPb_NUR%20HAJARATUL%20ASWAT%20KAMARUDDIN%20AS%20N%2014_5.pdf
first_indexed	2023-09-18T23:13:15Z
last_indexed	2023-09-18T23:13:15Z
_version_	1777418943524241408

Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin

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