Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan

Lead is metal that has very high toxicity and dangerous to human body. Once it had been absorbed in the body, it would deposit on vital organ such as brain and induce apoptosis, a process of cell death. This study aimed to examine the effect of lead toxicity on apoptosis of brain cells through light...

Full description

Bibliographic Details
Main Author:	Mhd Ngadisan, Syasya Aqilah
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2015
Subjects:	QR Microbiology Bacteria
Online Access:	http://ir.uitm.edu.my/id/eprint/24080/ http://ir.uitm.edu.my/id/eprint/24080/1/PPb_SYASYA%20AQILAH%20MHD%20NGADISAN%20AS%20N%2015_5.pdf

id	uitm-24080
recordtype	eprints
spelling	uitm-240802019-05-08T01:07:14Z http://ir.uitm.edu.my/id/eprint/24080/ Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan Mhd Ngadisan, Syasya Aqilah QR Microbiology Bacteria Lead is metal that has very high toxicity and dangerous to human body. Once it had been absorbed in the body, it would deposit on vital organ such as brain and induce apoptosis, a process of cell death. This study aimed to examine the effect of lead toxicity on apoptosis of brain cells through light microscopic examinations and at the same time, this study aimed to determine the morphological differences between normal cells and death cells. The brains were harvested from two rat groups, controlled group and lead-treated group. The rats in control group were fed with distilled water while rats of treated group were fed with distilled water and 0.2% lead acetate. 0.2% lead acetate was used in this study because previous research recorded that low level of lead exposure could induce cell apoptosis and may cause impairments in learning, memory and neurologic development especially in rats. All these brain samples were obtained from Dentistry Department, Uitm Shah Alam. The brains were sliced into extremely thin sections using microtome and stained using haematoxylin and eosin (H&E). The H&E staining is a staining method that had been widely used in histology to determine the structure of the cells. The haematoxylin stained the nucleus blue while eosin stained the cytoplasm with red colour. The result of this study demonstrated that low level of lead exposure could induce apoptosis in brain cells and it proved that H&E staining method is successful and reliable to observe and differentiate between normal and death cells. For future research, it is recommended to study the expression of genes that control the cell apoptosis for more precise and accurate assessment. The addition of more parameters helps to justify the validity of the results. Faculty of Applied Sciences 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24080/1/PPb_SYASYA%20AQILAH%20MHD%20NGADISAN%20AS%20N%2015_5.pdf Mhd Ngadisan, Syasya Aqilah (2015) Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology Bacteria
spellingShingle	QR Microbiology Bacteria Mhd Ngadisan, Syasya Aqilah Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
description	Lead is metal that has very high toxicity and dangerous to human body. Once it had been absorbed in the body, it would deposit on vital organ such as brain and induce apoptosis, a process of cell death. This study aimed to examine the effect of lead toxicity on apoptosis of brain cells through light microscopic examinations and at the same time, this study aimed to determine the morphological differences between normal cells and death cells. The brains were harvested from two rat groups, controlled group and lead-treated group. The rats in control group were fed with distilled water while rats of treated group were fed with distilled water and 0.2% lead acetate. 0.2% lead acetate was used in this study because previous research recorded that low level of lead exposure could induce cell apoptosis and may cause impairments in learning, memory and neurologic development especially in rats. All these brain samples were obtained from Dentistry Department, Uitm Shah Alam. The brains were sliced into extremely thin sections using microtome and stained using haematoxylin and eosin (H&E). The H&E staining is a staining method that had been widely used in histology to determine the structure of the cells. The haematoxylin stained the nucleus blue while eosin stained the cytoplasm with red colour. The result of this study demonstrated that low level of lead exposure could induce apoptosis in brain cells and it proved that H&E staining method is successful and reliable to observe and differentiate between normal and death cells. For future research, it is recommended to study the expression of genes that control the cell apoptosis for more precise and accurate assessment. The addition of more parameters helps to justify the validity of the results.
format	Student Project
author	Mhd Ngadisan, Syasya Aqilah
author_facet	Mhd Ngadisan, Syasya Aqilah
author_sort	Mhd Ngadisan, Syasya Aqilah
title	Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_short	Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_full	Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_fullStr	Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_full_unstemmed	Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_sort	histological study following lead exposure of sprague dawley brain / syasya aqilah mhd ngadisan
publisher	Faculty of Applied Sciences
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/24080/ http://ir.uitm.edu.my/id/eprint/24080/1/PPb_SYASYA%20AQILAH%20MHD%20NGADISAN%20AS%20N%2015_5.pdf
first_indexed	2023-09-18T23:11:50Z
last_indexed	2023-09-18T23:11:50Z
_version_	1777418853591023616

Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan

Similar Items