A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin

Little is known on the biohazard level of microorganisms in raw products. The main purpose of this study was to investigate the prevalence and quantification of Escherichia coli and Salmonella sp. in the raw coconut milk samples by using Most Probable Number-Polymerase Chain Reaction (MPN-PCR) metho...

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Main Author:	Jalaluddin, Noor Syafiqah
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2015
Subjects:	QR Microbiology Bacteria
Online Access:	http://ir.uitm.edu.my/id/eprint/23906/ http://ir.uitm.edu.my/id/eprint/23906/1/PPb_NOOR%20SYAFIQAH%20JALALUDDIN%20AS%20N%2015_5.pdf

id	uitm-23906
recordtype	eprints
spelling	uitm-239062019-04-22T02:06:27Z http://ir.uitm.edu.my/id/eprint/23906/ A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin Jalaluddin, Noor Syafiqah QR Microbiology Bacteria Little is known on the biohazard level of microorganisms in raw products. The main purpose of this study was to investigate the prevalence and quantification of Escherichia coli and Salmonella sp. in the raw coconut milk samples by using Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. A total of ten samples of raw coconut milks were purchased randomly from several wet market and hypermarket in Kuala Pilah and Senawang areas. A total of 10% (1/10) samples from both markets showed positive result of Escherichia coli while 100% (10/10) samples showed positive result of Salmonella sp. The estimated quantities of positive turbid tubes from the wet market were ranging from 2.0x105 MPN/g to 2.4x107 MPN/g. The estimated quantities of positive turbid tubes from hypermarket were ranging from 2.8x105 MPN/g to 2.4x107 MPN/g. In this study, the presumptive amount of Escherichia coli in wet market samples was ranging from 3.0x104 MPN/g to 9.4x104 MPN/g while Salmonella sp. was ranging from 3.0x104 MPN/g to 4.4x105 MPN/g. Based on the result, it showed that Escherichia coli did not occur in hypermarket samples. Meanwhile, Salmonella sp. was ranging from 3.0x104 MPN/g to 2.4x107 MPN/g. The outcome of this study showed that the presence of microbial contaminants that indicates poor milk quality, which requires immediate consideration as it can pose serious health risk to consumers. Faculty of Applied Sciences 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/23906/1/PPb_NOOR%20SYAFIQAH%20JALALUDDIN%20AS%20N%2015_5.pdf Jalaluddin, Noor Syafiqah (2015) A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin. [Student Project] (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology Bacteria
spellingShingle	QR Microbiology Bacteria Jalaluddin, Noor Syafiqah A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin
description	Little is known on the biohazard level of microorganisms in raw products. The main purpose of this study was to investigate the prevalence and quantification of Escherichia coli and Salmonella sp. in the raw coconut milk samples by using Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. A total of ten samples of raw coconut milks were purchased randomly from several wet market and hypermarket in Kuala Pilah and Senawang areas. A total of 10% (1/10) samples from both markets showed positive result of Escherichia coli while 100% (10/10) samples showed positive result of Salmonella sp. The estimated quantities of positive turbid tubes from the wet market were ranging from 2.0x105 MPN/g to 2.4x107 MPN/g. The estimated quantities of positive turbid tubes from hypermarket were ranging from 2.8x105 MPN/g to 2.4x107 MPN/g. In this study, the presumptive amount of Escherichia coli in wet market samples was ranging from 3.0x104 MPN/g to 9.4x104 MPN/g while Salmonella sp. was ranging from 3.0x104 MPN/g to 4.4x105 MPN/g. Based on the result, it showed that Escherichia coli did not occur in hypermarket samples. Meanwhile, Salmonella sp. was ranging from 3.0x104 MPN/g to 2.4x107 MPN/g. The outcome of this study showed that the presence of microbial contaminants that indicates poor milk quality, which requires immediate consideration as it can pose serious health risk to consumers.
format	Student Project
author	Jalaluddin, Noor Syafiqah
author_facet	Jalaluddin, Noor Syafiqah
author_sort	Jalaluddin, Noor Syafiqah
title	A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin
title_short	A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin
title_full	A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin
title_fullStr	A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin
title_full_unstemmed	A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin
title_sort	detection of escherichia coli and salmonella sp. in raw coconut milk using mpn-pcr method / noor syafiqah jalaluddin
publisher	Faculty of Applied Sciences
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/23906/ http://ir.uitm.edu.my/id/eprint/23906/1/PPb_NOOR%20SYAFIQAH%20JALALUDDIN%20AS%20N%2015_5.pdf
first_indexed	2023-09-18T23:11:34Z
last_indexed	2023-09-18T23:11:34Z
_version_	1777418837613871104

A detection of escherichia coli and salmonella sp. In raw coconut milk using MPN-PCR method / Noor Syafiqah Jalaluddin

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