Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi

This research project was aimed to isolate a pool of bacteria from pond soil of UiTM Negeri Sembilan, Kuala Pilah Campus using serial dilution followed by the screening of antagonism activities of the non-hemolytic isolates and to identify gram positive bacteria. This research involved collection of...

Full description

Bibliographic Details
Main Author: Mursidi, Fathin Amirah
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2014
Subjects:
Online Access:http://ir.uitm.edu.my/id/eprint/23561/
http://ir.uitm.edu.my/id/eprint/23561/1/PPb_FATHIN%20AMIRAH%20MURSIDI%20AS%20N%2014_5.pdf
id uitm-23561
recordtype eprints
spelling uitm-235612019-04-10T08:15:12Z http://ir.uitm.edu.my/id/eprint/23561/ Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi Mursidi, Fathin Amirah QR Microbiology Bacteria This research project was aimed to isolate a pool of bacteria from pond soil of UiTM Negeri Sembilan, Kuala Pilah Campus using serial dilution followed by the screening of antagonism activities of the non-hemolytic isolates and to identify gram positive bacteria. This research involved collection of soil sample, isolation of soil bacteria, purifying, preliminary screening of hemolytic activity, antagonism test by well diffusion method and identification of gram positive bacteria by gram staining. 80 different bacteria have been successfully isolated. Investigation of the antagonism activities revealed that only five non-hemolytic gram positive soil bacteria which were B6, B9, B32, B64, and B79 have the ability to act as antagonist against pathogenic bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. The isolates were discovered to be highly active against S. aureus followed by P. Aeruginosa. E. coli was the most resistant bacterial pathogen since none of the isolates found to be active against this pathogen. This significant finding suggest that pond soil in UiTM Negeri Sembilan, Kuala Pilah Campus may be a common source for the isolation of novel antimicrobials that are clinically and agriculturally important. Faculty of Applied Sciences 2014 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/23561/1/PPb_FATHIN%20AMIRAH%20MURSIDI%20AS%20N%2014_5.pdf Mursidi, Fathin Amirah (2014) Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi. [Student Project] (Submitted)
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic QR Microbiology
Bacteria
spellingShingle QR Microbiology
Bacteria
Mursidi, Fathin Amirah
Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi
description This research project was aimed to isolate a pool of bacteria from pond soil of UiTM Negeri Sembilan, Kuala Pilah Campus using serial dilution followed by the screening of antagonism activities of the non-hemolytic isolates and to identify gram positive bacteria. This research involved collection of soil sample, isolation of soil bacteria, purifying, preliminary screening of hemolytic activity, antagonism test by well diffusion method and identification of gram positive bacteria by gram staining. 80 different bacteria have been successfully isolated. Investigation of the antagonism activities revealed that only five non-hemolytic gram positive soil bacteria which were B6, B9, B32, B64, and B79 have the ability to act as antagonist against pathogenic bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. The isolates were discovered to be highly active against S. aureus followed by P. Aeruginosa. E. coli was the most resistant bacterial pathogen since none of the isolates found to be active against this pathogen. This significant finding suggest that pond soil in UiTM Negeri Sembilan, Kuala Pilah Campus may be a common source for the isolation of novel antimicrobials that are clinically and agriculturally important.
format Student Project
author Mursidi, Fathin Amirah
author_facet Mursidi, Fathin Amirah
author_sort Mursidi, Fathin Amirah
title Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi
title_short Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi
title_full Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi
title_fullStr Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi
title_full_unstemmed Screening of potential bacteria with antagonism properties isolated from pond soil in forest of UiTM Negeri Sembilan, Kuala Pilah Campus / Fathin Amirah Mursidi
title_sort screening of potential bacteria with antagonism properties isolated from pond soil in forest of uitm negeri sembilan, kuala pilah campus / fathin amirah mursidi
publisher Faculty of Applied Sciences
publishDate 2014
url http://ir.uitm.edu.my/id/eprint/23561/
http://ir.uitm.edu.my/id/eprint/23561/1/PPb_FATHIN%20AMIRAH%20MURSIDI%20AS%20N%2014_5.pdf
first_indexed 2023-09-18T23:10:56Z
last_indexed 2023-09-18T23:10:56Z
_version_ 1777418797232160768