Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar

The objectives of this study are to extract the volatile compounds in peppermint leaf by using SPME methods, to identify the major volatile compounds in peppermint leaf by using GC-MSD, to determine the optimum SPME condition and to evaluate if the SPME technique is feasible to be used as an extract...

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Main Author:	Jaafar, Noor Farhan
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2008
Subjects:	Aromatic compounds Analytical chemistry
Online Access:	http://ir.uitm.edu.my/id/eprint/23133/ http://ir.uitm.edu.my/id/eprint/23133/1/PPb_NOOR%20FARHAN%20JAAFAR%20AS%2008_5.pdf

id	uitm-23133
recordtype	eprints
spelling	uitm-231332019-02-25T06:58:35Z http://ir.uitm.edu.my/id/eprint/23133/ Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar Jaafar, Noor Farhan Aromatic compounds Analytical chemistry The objectives of this study are to extract the volatile compounds in peppermint leaf by using SPME methods, to identify the major volatile compounds in peppermint leaf by using GC-MSD, to determine the optimum SPME condition and to evaluate if the SPME technique is feasible to be used as an extraction technique for peppermint analysis using GC-MSD. The volatile compounds of peppermint leaves were analyzed using SPME technique combined with GCMSD (Agilent gas chromatography model 6890N coupled to a mass selective detector 5973 inert). Compounds were separated on a cross-linked fused silica capillary column HPS-MS (30m x 250\|im x 0.25\|im). The head pressure of the carrier gas helium (high purity, 99.9999%) was 50 kPa. SPME was done using 85\|iim polyacrylate (PA) fiber (Supelco. Inc.Bellefonte, PA, USA). The fiber was exposed to the sample headspace for 10 minutes at 45°C and immediately transferred onto the GC injection port with desorption time of 5 minutes at 250°C. GC-MSD analysis of the components of peppermint leaves revealed the presence of major compounds of peppermint such 2-Cyclohexen-l-one, 2-methyl-5- methylethenyl, Cyclohexanone, 2,3-Diaminobut-2-enedinitrile, Acetonitrile, 2- (2H-tetrazol-2-yl), 1-butyne, 4-methoxy, 1,3-Cyclobutanedicarbonitrile, Silane, Phenyl and 1,2-Cyclooctadiene. The aromatic or volatile compounds were identified by matching their mass spectra with the aromatic compounds spectral library. In the determination of the optimum SPME condition, three important parameters was considered such as extraction temperature, extraction time and desorption time. For optimized extraction temperature, the temperature values that were chosen for analysis are 50°C, 60°C and 70°C as variables parameter. To determine the optimum extraction time, three readings of extraction time was chosen for analysis which is 6, 8 and 10 minutes as variables parameter. For optimized desorption time, three values of desorption time that were chosen as variables parameter which 3, 5 and 7 minutes. From this analysis, the optimum extraction temperature and time that was obtained is 60°C and 8 minutes respectively. For desorption time, the time were obtained is 5 minutes. The using of SPME coupled with GC-MSD, the profile of volatile components in peppermint leaves can be easily identified. SPME method offers important advantage in the analysis of leaves sample namely fast, simple and solvent free. Faculty of Applied Sciences 2008 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/23133/1/PPb_NOOR%20FARHAN%20JAAFAR%20AS%2008_5.pdf Jaafar, Noor Farhan (2008) Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Aromatic compounds Analytical chemistry
spellingShingle	Aromatic compounds Analytical chemistry Jaafar, Noor Farhan Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar
description	The objectives of this study are to extract the volatile compounds in peppermint leaf by using SPME methods, to identify the major volatile compounds in peppermint leaf by using GC-MSD, to determine the optimum SPME condition and to evaluate if the SPME technique is feasible to be used as an extraction technique for peppermint analysis using GC-MSD. The volatile compounds of peppermint leaves were analyzed using SPME technique combined with GCMSD (Agilent gas chromatography model 6890N coupled to a mass selective detector 5973 inert). Compounds were separated on a cross-linked fused silica capillary column HPS-MS (30m x 250\|im x 0.25\|im). The head pressure of the carrier gas helium (high purity, 99.9999%) was 50 kPa. SPME was done using 85\|iim polyacrylate (PA) fiber (Supelco. Inc.Bellefonte, PA, USA). The fiber was exposed to the sample headspace for 10 minutes at 45°C and immediately transferred onto the GC injection port with desorption time of 5 minutes at 250°C. GC-MSD analysis of the components of peppermint leaves revealed the presence of major compounds of peppermint such 2-Cyclohexen-l-one, 2-methyl-5- methylethenyl, Cyclohexanone, 2,3-Diaminobut-2-enedinitrile, Acetonitrile, 2- (2H-tetrazol-2-yl), 1-butyne, 4-methoxy, 1,3-Cyclobutanedicarbonitrile, Silane, Phenyl and 1,2-Cyclooctadiene. The aromatic or volatile compounds were identified by matching their mass spectra with the aromatic compounds spectral library. In the determination of the optimum SPME condition, three important parameters was considered such as extraction temperature, extraction time and desorption time. For optimized extraction temperature, the temperature values that were chosen for analysis are 50°C, 60°C and 70°C as variables parameter. To determine the optimum extraction time, three readings of extraction time was chosen for analysis which is 6, 8 and 10 minutes as variables parameter. For optimized desorption time, three values of desorption time that were chosen as variables parameter which 3, 5 and 7 minutes. From this analysis, the optimum extraction temperature and time that was obtained is 60°C and 8 minutes respectively. For desorption time, the time were obtained is 5 minutes. The using of SPME coupled with GC-MSD, the profile of volatile components in peppermint leaves can be easily identified. SPME method offers important advantage in the analysis of leaves sample namely fast, simple and solvent free.
format	Student Project
author	Jaafar, Noor Farhan
author_facet	Jaafar, Noor Farhan
author_sort	Jaafar, Noor Farhan
title	Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar
title_short	Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar
title_full	Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar
title_fullStr	Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar
title_full_unstemmed	Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar
title_sort	analysis of volatile compounds in peppermint by using solid phase microextraction (spme) technique and gas chromatography-mass spectrometry (gc-ms) / noor farhan jaafar
publisher	Faculty of Applied Sciences
publishDate	2008
url	http://ir.uitm.edu.my/id/eprint/23133/ http://ir.uitm.edu.my/id/eprint/23133/1/PPb_NOOR%20FARHAN%20JAAFAR%20AS%2008_5.pdf
first_indexed	2023-09-18T23:10:08Z
last_indexed	2023-09-18T23:10:08Z
_version_	1777418746763149312

Analysis of volatile compounds in peppermint by using solid phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) / Noor Farhan Jaafar

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