Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin

The extract of Octomeles Sumatranus has been used by the Sarawak and Sabah natives as health tonic. An attempt has been made to chemically profile the extract using LC-MS but the types of flavonoids cannot be determined since the flavonoids glycosides compounds contain many sugars. Therefore, the gl...

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Main Author:	Nordin, Farah Ad-Din
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2009
Subjects:	Oxidation Biochemistry
Online Access:	http://ir.uitm.edu.my/id/eprint/22942/ http://ir.uitm.edu.my/id/eprint/22942/1/PPb_FARAH%20AD-DIN%20NORDIN%20AS%2009_5.pdf

id	uitm-22942
recordtype	eprints
spelling	uitm-229422019-03-06T03:41:25Z http://ir.uitm.edu.my/id/eprint/22942/ Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin Nordin, Farah Ad-Din Oxidation Biochemistry The extract of Octomeles Sumatranus has been used by the Sarawak and Sabah natives as health tonic. An attempt has been made to chemically profile the extract using LC-MS but the types of flavonoids cannot be determined since the flavonoids glycosides compounds contain many sugars. Therefore, the glycosides were hydrolysed for 3 hours at 80°C and the resulting aglycones were identified using LCMS. The conditions for analyzing the aglycones at HPLC and LC-MS were the same. The hydrolysis was a success based on the differences from the retention time of the unhydrolyzed and hydrolyzed extract. The Rt of the unhydrolyzed extract was 15 minutes while the Rt of the hydrolyzed extract was 30 minutes. The hydrolyzed extract was later being submitted to LC-MS for identification and structural determination of the aglycones. The MH+ peak was m/z 365.26 which shows the possible molecular weight of the aglycones. The antioxidant activities of Octomeles Sumatranus were investigated based on free radical scavenging activity, DPPH. The IC50 of the unhydrolyzed extract was detectable at 517 nm and the value was determined to be 19 \|ig/mL. Faculty of Applied Sciences 2009 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/22942/1/PPb_FARAH%20AD-DIN%20NORDIN%20AS%2009_5.pdf Nordin, Farah Ad-Din (2009) Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Oxidation Biochemistry
spellingShingle	Oxidation Biochemistry Nordin, Farah Ad-Din Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin
description	The extract of Octomeles Sumatranus has been used by the Sarawak and Sabah natives as health tonic. An attempt has been made to chemically profile the extract using LC-MS but the types of flavonoids cannot be determined since the flavonoids glycosides compounds contain many sugars. Therefore, the glycosides were hydrolysed for 3 hours at 80°C and the resulting aglycones were identified using LCMS. The conditions for analyzing the aglycones at HPLC and LC-MS were the same. The hydrolysis was a success based on the differences from the retention time of the unhydrolyzed and hydrolyzed extract. The Rt of the unhydrolyzed extract was 15 minutes while the Rt of the hydrolyzed extract was 30 minutes. The hydrolyzed extract was later being submitted to LC-MS for identification and structural determination of the aglycones. The MH+ peak was m/z 365.26 which shows the possible molecular weight of the aglycones. The antioxidant activities of Octomeles Sumatranus were investigated based on free radical scavenging activity, DPPH. The IC50 of the unhydrolyzed extract was detectable at 517 nm and the value was determined to be 19 \|ig/mL.
format	Student Project
author	Nordin, Farah Ad-Din
author_facet	Nordin, Farah Ad-Din
author_sort	Nordin, Farah Ad-Din
title	Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin
title_short	Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin
title_full	Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin
title_fullStr	Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin
title_full_unstemmed	Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin
title_sort	hydrolysis of flavonoid glycosides of aqueous extract of octomeles sumatranus and its antioxidant activities / farah ad-din nordin
publisher	Faculty of Applied Sciences
publishDate	2009
url	http://ir.uitm.edu.my/id/eprint/22942/ http://ir.uitm.edu.my/id/eprint/22942/1/PPb_FARAH%20AD-DIN%20NORDIN%20AS%2009_5.pdf
first_indexed	2023-09-18T23:09:45Z
last_indexed	2023-09-18T23:09:45Z
_version_	1777418722503294976

Hydrolysis of flavonoid glycosides of aqueous extract of Octomeles Sumatranus and its antioxidant activities / Farah Ad-Din Nordin

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