Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari

Barringtonia racemosa or "Putat Kampung" was known to show various types of biological activities such as analgesic, antitumor and antimicrobial activities. B. racemosa has several secondary. metabolites which included the diterpenes, triterpenoids, flavonoids, steroids and saponins. Ch...

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Main Author:	Awari, Nur Azwani
Format:	Student Project
Language:	English
Published:	Faculty of Applied Sciences 2017
Subjects:	Plant anatomy Plant physiology
Online Access:	http://ir.uitm.edu.my/id/eprint/22883/ http://ir.uitm.edu.my/id/eprint/22883/1/PPb_NUR%20AZWANI%20AWARI%20AS%20C%2017_5.PDF

id	uitm-22883
recordtype	eprints
spelling	uitm-228832019-01-23T08:26:50Z http://ir.uitm.edu.my/id/eprint/22883/ Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari Awari, Nur Azwani Plant anatomy Plant physiology Barringtonia racemosa or "Putat Kampung" was known to show various types of biological activities such as analgesic, antitumor and antimicrobial activities. B. racemosa has several secondary. metabolites which included the diterpenes, triterpenoids, flavonoids, steroids and saponins. Chemical-based medicines mainly give unwanted side effects to the consumers. The microbial resistance towards these antibiotics also keep increasing. Therefore, the search for new potential effective plants to fight pathogenic microorganisms was timely to do. This study was carried out to determine the phytochemical compound in B. racemosa's methanol and hexane leaves extract, to identify the antimicrobial activities of B. racemosa leaves extract against selected Gram-positive and Gram-negative bacteria and to determine the minimum inhibitory concentration (MIC). The bacteria used were Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae and Pseudomonas aeruginosa. In this study, the antimicrobial activities of methanol and hexane extracts of B. racemosa were determined using disc diffusion and MIC tests. In phytochemical screening, alkaloids, flavonoids, tannins, terpenoids and saponins compounds were examined. 400 mg/ml, 200 mg/ml, 100 mg/ml and 50 mg/ml concentrations were used in determining the inhibition zones in disc diffusion method while 100 mg/ml, 50 mg/ml, 25 mg/ml and 12.5 mg/ml concentrations were used in MIC test. The results showed the presence of alkaloids, flavonoids, tannins, terpenoids and saponins in methanol extract while in hexane only tannins was present. For disc diffusion, the methanol extract showed the highest antimicrobial activity against P. aeruginosa at 400 mg/ml concentration while for hexane extract, there was no antimicrobial activity were detected against all bacteria tested. The concentration at 12.5 mg/ml was determined as the MIC for methanol extract. As a conclusion, B. racemosa has a promising high potential as an antibacterial agent and be able to replace the existing chemical-based products Faculty of Applied Sciences 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/22883/1/PPb_NUR%20AZWANI%20AWARI%20AS%20C%2017_5.PDF Awari, Nur Azwani (2017) Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari. [Student Project] (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Plant anatomy Plant physiology
spellingShingle	Plant anatomy Plant physiology Awari, Nur Azwani Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari
description	Barringtonia racemosa or "Putat Kampung" was known to show various types of biological activities such as analgesic, antitumor and antimicrobial activities. B. racemosa has several secondary. metabolites which included the diterpenes, triterpenoids, flavonoids, steroids and saponins. Chemical-based medicines mainly give unwanted side effects to the consumers. The microbial resistance towards these antibiotics also keep increasing. Therefore, the search for new potential effective plants to fight pathogenic microorganisms was timely to do. This study was carried out to determine the phytochemical compound in B. racemosa's methanol and hexane leaves extract, to identify the antimicrobial activities of B. racemosa leaves extract against selected Gram-positive and Gram-negative bacteria and to determine the minimum inhibitory concentration (MIC). The bacteria used were Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae and Pseudomonas aeruginosa. In this study, the antimicrobial activities of methanol and hexane extracts of B. racemosa were determined using disc diffusion and MIC tests. In phytochemical screening, alkaloids, flavonoids, tannins, terpenoids and saponins compounds were examined. 400 mg/ml, 200 mg/ml, 100 mg/ml and 50 mg/ml concentrations were used in determining the inhibition zones in disc diffusion method while 100 mg/ml, 50 mg/ml, 25 mg/ml and 12.5 mg/ml concentrations were used in MIC test. The results showed the presence of alkaloids, flavonoids, tannins, terpenoids and saponins in methanol extract while in hexane only tannins was present. For disc diffusion, the methanol extract showed the highest antimicrobial activity against P. aeruginosa at 400 mg/ml concentration while for hexane extract, there was no antimicrobial activity were detected against all bacteria tested. The concentration at 12.5 mg/ml was determined as the MIC for methanol extract. As a conclusion, B. racemosa has a promising high potential as an antibacterial agent and be able to replace the existing chemical-based products
format	Student Project
author	Awari, Nur Azwani
author_facet	Awari, Nur Azwani
author_sort	Awari, Nur Azwani
title	Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari
title_short	Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari
title_full	Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari
title_fullStr	Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari
title_full_unstemmed	Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari
title_sort	phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / nur azwani awari
publisher	Faculty of Applied Sciences
publishDate	2017
url	http://ir.uitm.edu.my/id/eprint/22883/ http://ir.uitm.edu.my/id/eprint/22883/1/PPb_NUR%20AZWANI%20AWARI%20AS%20C%2017_5.PDF
first_indexed	2023-09-18T23:09:36Z
last_indexed	2023-09-18T23:09:36Z
_version_	1777418713914408960

Phytochemical screening and antimicrobial actmties of barringtonia racemosa leaves against selected bacteria / Nur Azwani Awari

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