Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi

Special attention has been given to the second species of oil palm, Elaeis oleifera as it possess several interesting agronomic traits such as slow growth, higher oil unsaturation and disease resistance. Studying the variability of E. oleifera germplasm is therefore very important as it serves as a...

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Main Author:	Wan Hanafi, Wan Nurhayati
Format:	Book Section
Language:	English
Published:	Institute of Graduate Studies, UiTM 2018
Subjects:	Palm oil
Online Access:	http://ir.uitm.edu.my/id/eprint/22095/ http://ir.uitm.edu.my/id/eprint/22095/1/ABS_WAN%20NURHAYATI%20WAN%20HANAFI%20TDRA%20VOL%2014%20IGS%2018.pdf

id	uitm-22095
recordtype	eprints
spelling	uitm-220952018-11-13T08:26:51Z http://ir.uitm.edu.my/id/eprint/22095/ Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi Wan Hanafi, Wan Nurhayati Palm oil Special attention has been given to the second species of oil palm, Elaeis oleifera as it possess several interesting agronomic traits such as slow growth, higher oil unsaturation and disease resistance. Studying the variability of E. oleifera germplasm is therefore very important as it serves as a tool to select source of genetic diversity for the oil palm conservation programme. The objectives of this study were 1) to identify the polymorphic E. oleifera gSSRs for E. oleifera, 2) to measure the information content of E. oleifera gSSRs, 3) to unravel the genetic diversity of E. oleifera germplasm, 4) to determine the genetic differentiation of E. oleifera germplasm and 5) to assess the genetic structure of E. oleifera germplasm. MPOB has developed a collection of simple sequence repeats (SSRs) from E. oleifera genome. Initially, optimization of PCR conditions for analysis of the oleifera samples using E. oleifera gSSRs was carried out. A total of 316 E. oleifera gSSRs were tested to evaluate their usefulness to assess the genetic diversity and population structure of E. oleifera populations. The PCR conditions were optimized while keeping the original DNA concentration, annealing temperature (TA) and other reagent constant. Out of 316 E. oleifera gSSRs screened, 270 produced amplicons and of these numbers, 140 were polymorphic and potentially useful for diversity analysis. The modified PCR condition increases the success of amplifying E. oleifera gSSRs in the E. oleifera DNA samples analyzed. The PCR methods together with the polymorphic E. oleifera gSSRs were applied in genotyping the entire E. oleifera populations. A set of 21 polymorphic E. oleifera gSSRs was analyzed on a total of 214 E. oleifera genomic DNA belonging to eight germplasm originated from four countries in Central and South America (Columbia, Panama, Costa Rica and Honduras)… Institute of Graduate Studies, UiTM 2018 Book Section PeerReviewed text en http://ir.uitm.edu.my/id/eprint/22095/1/ABS_WAN%20NURHAYATI%20WAN%20HANAFI%20TDRA%20VOL%2014%20IGS%2018.pdf Wan Hanafi, Wan Nurhayati (2018) Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi. In: The Doctoral Research Abstracts. IGS Biannual Publication, 14 . Institute of Graduate Studies, UiTM, Shah Alam.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Palm oil
spellingShingle	Palm oil Wan Hanafi, Wan Nurhayati Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi
description	Special attention has been given to the second species of oil palm, Elaeis oleifera as it possess several interesting agronomic traits such as slow growth, higher oil unsaturation and disease resistance. Studying the variability of E. oleifera germplasm is therefore very important as it serves as a tool to select source of genetic diversity for the oil palm conservation programme. The objectives of this study were 1) to identify the polymorphic E. oleifera gSSRs for E. oleifera, 2) to measure the information content of E. oleifera gSSRs, 3) to unravel the genetic diversity of E. oleifera germplasm, 4) to determine the genetic differentiation of E. oleifera germplasm and 5) to assess the genetic structure of E. oleifera germplasm. MPOB has developed a collection of simple sequence repeats (SSRs) from E. oleifera genome. Initially, optimization of PCR conditions for analysis of the oleifera samples using E. oleifera gSSRs was carried out. A total of 316 E. oleifera gSSRs were tested to evaluate their usefulness to assess the genetic diversity and population structure of E. oleifera populations. The PCR conditions were optimized while keeping the original DNA concentration, annealing temperature (TA) and other reagent constant. Out of 316 E. oleifera gSSRs screened, 270 produced amplicons and of these numbers, 140 were polymorphic and potentially useful for diversity analysis. The modified PCR condition increases the success of amplifying E. oleifera gSSRs in the E. oleifera DNA samples analyzed. The PCR methods together with the polymorphic E. oleifera gSSRs were applied in genotyping the entire E. oleifera populations. A set of 21 polymorphic E. oleifera gSSRs was analyzed on a total of 214 E. oleifera genomic DNA belonging to eight germplasm originated from four countries in Central and South America (Columbia, Panama, Costa Rica and Honduras)…
format	Book Section
author	Wan Hanafi, Wan Nurhayati
author_facet	Wan Hanafi, Wan Nurhayati
author_sort	Wan Hanafi, Wan Nurhayati
title	Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi
title_short	Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi
title_full	Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi
title_fullStr	Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi
title_full_unstemmed	Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi
title_sort	evaluation of the simple sequence repeat (ssr) genotyping of elaeis oleifera germplasm / wan nurhayati wan hanafi
publisher	Institute of Graduate Studies, UiTM
publishDate	2018
url	http://ir.uitm.edu.my/id/eprint/22095/ http://ir.uitm.edu.my/id/eprint/22095/1/ABS_WAN%20NURHAYATI%20WAN%20HANAFI%20TDRA%20VOL%2014%20IGS%2018.pdf
first_indexed	2023-09-18T23:07:55Z
last_indexed	2023-09-18T23:07:55Z
_version_	1777418607300444160

Evaluation of the simple sequence repeat (SSR) genotyping of Elaeis oleifera germplasm / Wan Nurhayati Wan Hanafi

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