HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin

HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin

Bitter quassinoids have been reported to possess numerous biological activities. They are degraded triterpenoids classified according to their basic skeletons; C18, C19, C20, C22, C25. Currently, more than 200 quassinoids have been isolated from genus Quassia, Brucea, Soulamea, Eurycoma, Picrolemnia...

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Main Author: Ag Nuddin, Julenah
Format: Book Section
Language:English
Published: Institute of Graduate Studies, UiTM 2016
Subjects:
Malaysia
Online Access:http://ir.uitm.edu.my/id/eprint/19917/
http://ir.uitm.edu.my/id/eprint/19917/1/ABS_JULENAH%20AG%20NUDDIN%20TDRA%20VOL%2010%20IGS%2016.pdf
id uitm-19917
recordtype eprints
spelling uitm-199172018-06-08T08:14:34Z http://ir.uitm.edu.my/id/eprint/19917/ HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin Ag Nuddin, Julenah Malaysia Bitter quassinoids have been reported to possess numerous biological activities. They are degraded triterpenoids classified according to their basic skeletons; C18, C19, C20, C22, C25. Currently, more than 200 quassinoids have been isolated from genus Quassia, Brucea, Soulamea, Eurycoma, Picrolemnia and others. A study on Quassia borneensis Noot. (Simaroubaceae) (Qb) for its anti-proliferative active quassinoids using a developed and validated high performance liquid chromatography (HPLC) isolation method is appropriate for data gathering. In addition, a total synthesis exploration of canthin-6-one would provide information regarding its mechanism and influential factors for viability study. The study was accomplished by extracting powdered bark and root Qb by cold maceration and soxhlet in methanol for yield comparison. The methanol extracts were partitioned to acquire n-hexane, chloroform and aqueous fractions. They were tested for their anti-proliferative activity based on MTS assay using HL-60 cell line. Later, chloroform and aqueous fractions were subjected to isolation and purification with HPLC. Then, pure isolates were structurally elucidated with liquid chromatographymass spectroscopy (LC-MS) and nuclear magnetic resonance (NMR). In addition, the mass profile of active fractions was measured using liquid chromatography-mass spectrometer triple quadrupole time of flight (LC-MSQTOF). Lastly, pure quassinoid compounds were subjected to MTT assay… Institute of Graduate Studies, UiTM 2016 Book Section PeerReviewed text en http://ir.uitm.edu.my/id/eprint/19917/1/ABS_JULENAH%20AG%20NUDDIN%20TDRA%20VOL%2010%20IGS%2016.pdf Ag Nuddin, Julenah (2016) HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin. In: The Doctoral Research Abstracts. IGS Biannual Publication, 10 (10). Institute of Graduate Studies, UiTM, Shah Alam.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Malaysia
spellingShingle Malaysia
Ag Nuddin, Julenah
HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin
description Bitter quassinoids have been reported to possess numerous biological activities. They are degraded triterpenoids classified according to their basic skeletons; C18, C19, C20, C22, C25. Currently, more than 200 quassinoids have been isolated from genus Quassia, Brucea, Soulamea, Eurycoma, Picrolemnia and others. A study on Quassia borneensis Noot. (Simaroubaceae) (Qb) for its anti-proliferative active quassinoids using a developed and validated high performance liquid chromatography (HPLC) isolation method is appropriate for data gathering. In addition, a total synthesis exploration of canthin-6-one would provide information regarding its mechanism and influential factors for viability study. The study was accomplished by extracting powdered bark and root Qb by cold maceration and soxhlet in methanol for yield comparison. The methanol extracts were partitioned to acquire n-hexane, chloroform and aqueous fractions. They were tested for their anti-proliferative activity based on MTS assay using HL-60 cell line. Later, chloroform and aqueous fractions were subjected to isolation and purification with HPLC. Then, pure isolates were structurally elucidated with liquid chromatographymass spectroscopy (LC-MS) and nuclear magnetic resonance (NMR). In addition, the mass profile of active fractions was measured using liquid chromatography-mass spectrometer triple quadrupole time of flight (LC-MSQTOF). Lastly, pure quassinoid compounds were subjected to MTT assay…
format Book Section
author Ag Nuddin, Julenah
author_facet Ag Nuddin, Julenah
author_sort Ag Nuddin, Julenah
title HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin
title_short HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin
title_full HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin
title_fullStr HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin
title_full_unstemmed HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin
title_sort hplc method development & structural elucidation of anti-proliferative quassinoids from quassia borneensis noot. (simaroubaceae) / julenah ag nuddin
publisher Institute of Graduate Studies, UiTM
publishDate 2016
url http://ir.uitm.edu.my/id/eprint/19917/
http://ir.uitm.edu.my/id/eprint/19917/1/ABS_JULENAH%20AG%20NUDDIN%20TDRA%20VOL%2010%20IGS%2016.pdf
first_indexed 2023-09-18T23:03:34Z
last_indexed 2023-09-18T23:03:34Z
_version_ 1777418333910466560

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