The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli

The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli

Neuroprotective strategies are required to complement the available medical treatments in order to enhance the brain endogenous protective mechanisms and cushion the effect of stroke injury. Pharmacological preconditioning is an avenue of preventative medication anticipated to be highly effective in...

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Main Author: Nik Ramli, Nik Nasihah
Format: Book Section
Language:English
Published: Institute of Graduate Studies, UiTM 2017
Subjects:
Malaysia
Online Access:http://ir.uitm.edu.my/id/eprint/19786/
http://ir.uitm.edu.my/id/eprint/19786/1/ABS_NIK%20NASIHAH%20NIK%20RAMLI%20TDRA%20VOL%2011%20IGS%2017.pdf
id uitm-19786
recordtype eprints
spelling uitm-197862018-06-07T08:09:24Z http://ir.uitm.edu.my/id/eprint/19786/ The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli Nik Ramli, Nik Nasihah Malaysia Neuroprotective strategies are required to complement the available medical treatments in order to enhance the brain endogenous protective mechanisms and cushion the effect of stroke injury. Pharmacological preconditioning is an avenue of preventative medication anticipated to be highly effective in protecting and reducing the ischemic induced neuronal damage. Recently, in vitro preconditioning studies have shown that prior activation of group I metabotropic receptor (mGluR) with its specific agonist (S)- 3,5-dihydroxyphenylglycine ((S)-3,5-DHPG) elicits neuroprotection against excitotoxicity. Furthermore, the activation of group I mGluR regulates the expression of DREAM. DREAM protein regulates transcription of various genes including edem1 which is a component protein of ER-associated degradation pathway (ERAD). This study elucidates the neuroprotective effect of group I mGluR agonist preconditioning, (S)-3,5-DHPG via DREAM and ERAD in acute ischemic stroke rats. One, 10 or 100 μM (S)- 3,5-DHPG was administered intrathecally to 6 adult male Sprague Dawley rats 2 hours prior to the middle cerebral artery occlusion. After 24 hours, the modified neurological severity score (mNSS) and grid walking test were assessed. The rats were sacrificed and the infarct brain volumes were estimated by 2,3,5-triphenyltetrazolium chloride staining. The serum level of neuron-specific enolase (NSE) and brain tissue level of Bip/GRP78 ER stress marker were assessed by ELISA assays… Institute of Graduate Studies, UiTM 2017 Book Section PeerReviewed text en http://ir.uitm.edu.my/id/eprint/19786/1/ABS_NIK%20NASIHAH%20NIK%20RAMLI%20TDRA%20VOL%2011%20IGS%2017.pdf Nik Ramli, Nik Nasihah (2017) The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli. In: The Doctoral Research Abstracts. IGS Biannual Publication, 11 (11). Institute of Graduate Studies, UiTM, Shah Alam.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Malaysia
spellingShingle Malaysia
Nik Ramli, Nik Nasihah
The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli
description Neuroprotective strategies are required to complement the available medical treatments in order to enhance the brain endogenous protective mechanisms and cushion the effect of stroke injury. Pharmacological preconditioning is an avenue of preventative medication anticipated to be highly effective in protecting and reducing the ischemic induced neuronal damage. Recently, in vitro preconditioning studies have shown that prior activation of group I metabotropic receptor (mGluR) with its specific agonist (S)- 3,5-dihydroxyphenylglycine ((S)-3,5-DHPG) elicits neuroprotection against excitotoxicity. Furthermore, the activation of group I mGluR regulates the expression of DREAM. DREAM protein regulates transcription of various genes including edem1 which is a component protein of ER-associated degradation pathway (ERAD). This study elucidates the neuroprotective effect of group I mGluR agonist preconditioning, (S)-3,5-DHPG via DREAM and ERAD in acute ischemic stroke rats. One, 10 or 100 μM (S)- 3,5-DHPG was administered intrathecally to 6 adult male Sprague Dawley rats 2 hours prior to the middle cerebral artery occlusion. After 24 hours, the modified neurological severity score (mNSS) and grid walking test were assessed. The rats were sacrificed and the infarct brain volumes were estimated by 2,3,5-triphenyltetrazolium chloride staining. The serum level of neuron-specific enolase (NSE) and brain tissue level of Bip/GRP78 ER stress marker were assessed by ELISA assays…
format Book Section
author Nik Ramli, Nik Nasihah
author_facet Nik Ramli, Nik Nasihah
author_sort Nik Ramli, Nik Nasihah
title The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli
title_short The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli
title_full The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli
title_fullStr The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli
title_full_unstemmed The neuroprotective mechanism of dream via ERAD pathway In dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / Nik Nasihah Nik Ramli
title_sort neuroprotective mechanism of dream via erad pathway in dyhydroxyphenylglycine preconditioned acute ischemic stroke rats / nik nasihah nik ramli
publisher Institute of Graduate Studies, UiTM
publishDate 2017
url http://ir.uitm.edu.my/id/eprint/19786/
http://ir.uitm.edu.my/id/eprint/19786/1/ABS_NIK%20NASIHAH%20NIK%20RAMLI%20TDRA%20VOL%2011%20IGS%2017.pdf
first_indexed 2023-09-18T23:03:17Z
last_indexed 2023-09-18T23:03:17Z
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