Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz

Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz

The coagulase-negative Staphylococcus (CoNS) is a group of bacteria that are gaining prominence as emerging pathogens of hospital-acquired infections. One such species is S. capitis, which is now the major cause of bloodstream infection especially in neonatal intensive units. The major virulence fac...

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Main Author: Abd Aziz, Aziyah
Format: Book Section
Language:English
Published: Institute of Graduate Studies, UiTM 2015
Subjects:
Malaysia
Online Access:http://ir.uitm.edu.my/id/eprint/19431/
http://ir.uitm.edu.my/id/eprint/19431/1/ABS_AZIYAH%20ABD%20AZIZ%20TDRA%20VOL%208%20IGS%2015.pdf
id uitm-19431
recordtype eprints
spelling uitm-194312018-06-11T06:53:11Z http://ir.uitm.edu.my/id/eprint/19431/ Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz Abd Aziz, Aziyah Malaysia The coagulase-negative Staphylococcus (CoNS) is a group of bacteria that are gaining prominence as emerging pathogens of hospital-acquired infections. One such species is S. capitis, which is now the major cause of bloodstream infection especially in neonatal intensive units. The major virulence factor of S. capitis appears to be its ability to form a biofilm structure. A total of 200 local clinical isolates of CoNS was obtained from the Hospital Tuanku Ampuan Rahimah, Klang in between December 2010 to May 2011. Nine species of CoNS were identified with S. epidermidis, S. haemolyticus, S. hominis and S. capitis being the most prevalent strains. Identification of the isolates by biochemical tests using the Microgen Staph ID kit was less than 50% accurate while identification via the sodA gene sequence provided better discrimination and accuracy. The ERIC-PCR fingerprinting was then used to genotype the CoNS strains and the Discriminative Index (D) was calculated. At D = 0.949, ERIC-PCR can be used with confidence to discriminate between the S. hominis strains. However, low discriminative power (D < 0.9) was observed for S. capitis, S. epidermidis and S. haemolyticus implying that ERIC- PCR fingerprinting is not sufficient to genotype these strains. A multiplex PCR method was successfully developed to probe for the presence of icaABCD operon in a majority of the bacterial strains. At 88%, S. capitis showed the highest ability to form biofilm with a large percentage of these forming dense biofilm structures while the icaABCD operon was found to be present in all of the strains… Institute of Graduate Studies, UiTM 2015 Book Section PeerReviewed text en http://ir.uitm.edu.my/id/eprint/19431/1/ABS_AZIYAH%20ABD%20AZIZ%20TDRA%20VOL%208%20IGS%2015.pdf Abd Aziz, Aziyah (2015) Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz. In: The Doctoral Research Abstracts. IGS Biannual Publication, 8 (8). Institute of Graduate Studies, UiTM, Shah Alam.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Malaysia
spellingShingle Malaysia
Abd Aziz, Aziyah
Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz
description The coagulase-negative Staphylococcus (CoNS) is a group of bacteria that are gaining prominence as emerging pathogens of hospital-acquired infections. One such species is S. capitis, which is now the major cause of bloodstream infection especially in neonatal intensive units. The major virulence factor of S. capitis appears to be its ability to form a biofilm structure. A total of 200 local clinical isolates of CoNS was obtained from the Hospital Tuanku Ampuan Rahimah, Klang in between December 2010 to May 2011. Nine species of CoNS were identified with S. epidermidis, S. haemolyticus, S. hominis and S. capitis being the most prevalent strains. Identification of the isolates by biochemical tests using the Microgen Staph ID kit was less than 50% accurate while identification via the sodA gene sequence provided better discrimination and accuracy. The ERIC-PCR fingerprinting was then used to genotype the CoNS strains and the Discriminative Index (D) was calculated. At D = 0.949, ERIC-PCR can be used with confidence to discriminate between the S. hominis strains. However, low discriminative power (D < 0.9) was observed for S. capitis, S. epidermidis and S. haemolyticus implying that ERIC- PCR fingerprinting is not sufficient to genotype these strains. A multiplex PCR method was successfully developed to probe for the presence of icaABCD operon in a majority of the bacterial strains. At 88%, S. capitis showed the highest ability to form biofilm with a large percentage of these forming dense biofilm structures while the icaABCD operon was found to be present in all of the strains…
format Book Section
author Abd Aziz, Aziyah
author_facet Abd Aziz, Aziyah
author_sort Abd Aziz, Aziyah
title Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz
title_short Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz
title_full Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz
title_fullStr Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz
title_full_unstemmed Molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in S. capitis / Aziyah Abd Aziz
title_sort molecular characterization of coagulase-negative staphylococcus and biofilm- associated genes in s. capitis / aziyah abd aziz
publisher Institute of Graduate Studies, UiTM
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/19431/
http://ir.uitm.edu.my/id/eprint/19431/1/ABS_AZIYAH%20ABD%20AZIZ%20TDRA%20VOL%208%20IGS%2015.pdf
first_indexed 2023-09-18T23:02:33Z
last_indexed 2023-09-18T23:02:33Z
_version_ 1777418269877075968

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