Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif

Complete extraction of colorants often requires several steps and may use a mixture of several solvents. Pressurized liquid extraction (PLE) is an alternative method for colorants extraction. This study focuses on the influence of some operative parameters of PLE employed for the extraction of color...

Full description

Bibliographic Details
Main Author:	Sharif, Amirah
Format:	Thesis
Language:	English
Published:	2010
Online Access:	http://ir.uitm.edu.my/id/eprint/18287/ http://ir.uitm.edu.my/id/eprint/18287/2/TM_AMIRAH%20SHARIF%20AS%2010_5.pdf

id	uitm-18287
recordtype	eprints
spelling	uitm-182872019-02-27T06:47:10Z http://ir.uitm.edu.my/id/eprint/18287/ Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif Sharif, Amirah Complete extraction of colorants often requires several steps and may use a mixture of several solvents. Pressurized liquid extraction (PLE) is an alternative method for colorants extraction. This study focuses on the influence of some operative parameters of PLE employed for the extraction of colorants from onion (Allium cepa) skins. Extractions were conducted at temperatures ranging from 50 to 150 °C with a pressure of 1000 psi and two extraction cycles of 15 minutes. The effect of two extraction solvents (distilled water and 0.1 % HC1 in methanol (v/v)) was examined. It was found that extraction temperature and type of extraction solvent influenced the yield and color of extracted colorant, and the composition of anthocyanins extracted. Elevated temperatures were found to increase the yield significantly, while the effect of extraction pressure was found to be insignificant. In acidified methanol extraction, the yields (colorant (g) / 100 g sample) varied from 9.56 ± 0.46 to 16.71 ± 1.60 while using water; the yields varied from 7.06 ± 1.30 to 20.99 ± 0.75. Highest amount of yield was obtained at 150 °C for both acidified methanol and water extractions. However, the color of extract changes from red to brownish red as the extraction temperature increased probably due to degradation of anthocyanins. The color measurement indicated that PLE method using acidified methanol gave stronger reddish shade compared to that extracted using water. Major anthocyanins compounds identified include cyanidin 3-glucoside, cyanidin 3-rutinoside and cyanidin chloride and their composition depended on the extraction temperature and type of solvent used. Data showed that highest amount of identified anthocyanins were extracted at temperature of 80 °C using acidified methanol. Extracted cyanidin 3-glucoside, cyanidin 3-rutinoside and cyanidin chloride were 43.68 ± 0.24, 3.86 ± 0.04 and 1.55 ± 0.03 ppm, respectively. Using similar solvent, the yield and composition of anthocyanins obtained by conventional soaking method was comparable to those obtained using PLE at 80 °C. Colorant extract was successfully converted into conveniently handled powder by forming inclusion complexes with β-cyclodextrin. Selected powdered colorants were used for dyeing various types of fabric with different mordants. It was found that wool gave a broader range of shades. Based on washing fastness test, dyed wool showed good washing property. 2010 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/18287/2/TM_AMIRAH%20SHARIF%20AS%2010_5.pdf Sharif, Amirah (2010) Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
description	Complete extraction of colorants often requires several steps and may use a mixture of several solvents. Pressurized liquid extraction (PLE) is an alternative method for colorants extraction. This study focuses on the influence of some operative parameters of PLE employed for the extraction of colorants from onion (Allium cepa) skins. Extractions were conducted at temperatures ranging from 50 to 150 °C with a pressure of 1000 psi and two extraction cycles of 15 minutes. The effect of two extraction solvents (distilled water and 0.1 % HC1 in methanol (v/v)) was examined. It was found that extraction temperature and type of extraction solvent influenced the yield and color of extracted colorant, and the composition of anthocyanins extracted. Elevated temperatures were found to increase the yield significantly, while the effect of extraction pressure was found to be insignificant. In acidified methanol extraction, the yields (colorant (g) / 100 g sample) varied from 9.56 ± 0.46 to 16.71 ± 1.60 while using water; the yields varied from 7.06 ± 1.30 to 20.99 ± 0.75. Highest amount of yield was obtained at 150 °C for both acidified methanol and water extractions. However, the color of extract changes from red to brownish red as the extraction temperature increased probably due to degradation of anthocyanins. The color measurement indicated that PLE method using acidified methanol gave stronger reddish shade compared to that extracted using water. Major anthocyanins compounds identified include cyanidin 3-glucoside, cyanidin 3-rutinoside and cyanidin chloride and their composition depended on the extraction temperature and type of solvent used. Data showed that highest amount of identified anthocyanins were extracted at temperature of 80 °C using acidified methanol. Extracted cyanidin 3-glucoside, cyanidin 3-rutinoside and cyanidin chloride were 43.68 ± 0.24, 3.86 ± 0.04 and 1.55 ± 0.03 ppm, respectively. Using similar solvent, the yield and composition of anthocyanins obtained by conventional soaking method was comparable to those obtained using PLE at 80 °C. Colorant extract was successfully converted into conveniently handled powder by forming inclusion complexes with β-cyclodextrin. Selected powdered colorants were used for dyeing various types of fabric with different mordants. It was found that wool gave a broader range of shades. Based on washing fastness test, dyed wool showed good washing property.
format	Thesis
author	Sharif, Amirah
spellingShingle	Sharif, Amirah Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif
author_facet	Sharif, Amirah
author_sort	Sharif, Amirah
title	Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif
title_short	Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif
title_full	Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif
title_fullStr	Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif
title_full_unstemmed	Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif
title_sort	extraction of natural colorant from onion (allium cepa) skins using pressurized liquid extraction (ple) for application in fabric dyeing / amirah sharif
publishDate	2010
url	http://ir.uitm.edu.my/id/eprint/18287/ http://ir.uitm.edu.my/id/eprint/18287/2/TM_AMIRAH%20SHARIF%20AS%2010_5.pdf
first_indexed	2023-09-18T23:00:11Z
last_indexed	2023-09-18T23:00:11Z
_version_	1777418120802074624

Extraction of natural colorant from onion (Allium cepa) skins using Pressurized Liquid Extraction (PLE) for application in fabric dyeing / Amirah Sharif

Similar Items