Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian

In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), et...

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Main Author:	Sufian, Adila Sahida
Format:	Thesis
Language:	English
Published:	2013
Online Access:	http://ir.uitm.edu.my/id/eprint/18282/ http://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf

id	uitm-18282
recordtype	eprints
spelling	uitm-182822019-02-07T00:16:10Z http://ir.uitm.edu.my/id/eprint/18282/ Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian Sufian, Adila Sahida In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), ethyl acetate (MCEA) and aqueous (MCAQ)] were subjected to antimicrobial and cytotoxic activities using micro-broth dilution and MTT assays, respectively. MCEA, which appeared to be the most active extract against MSS A (S. aureus 25923) (MIC - 125 μg/mL) and HL60 cell line (human acute promyelocytic leukemia) (IC50 = 17.26 μg/mL), was further fractionated using vacuum liquid chromatography (VLC) to afford seven fractions (F1-F7). 2013 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf Sufian, Adila Sahida (2013) Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
description	In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), ethyl acetate (MCEA) and aqueous (MCAQ)] were subjected to antimicrobial and cytotoxic activities using micro-broth dilution and MTT assays, respectively. MCEA, which appeared to be the most active extract against MSS A (S. aureus 25923) (MIC - 125 μg/mL) and HL60 cell line (human acute promyelocytic leukemia) (IC50 = 17.26 μg/mL), was further fractionated using vacuum liquid chromatography (VLC) to afford seven fractions (F1-F7).
format	Thesis
author	Sufian, Adila Sahida
spellingShingle	Sufian, Adila Sahida Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
author_facet	Sufian, Adila Sahida
author_sort	Sufian, Adila Sahida
title	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_short	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_full	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_fullStr	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_full_unstemmed	Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
title_sort	bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of muntingia calabura / adila sahida sufian
publishDate	2013
url	http://ir.uitm.edu.my/id/eprint/18282/ http://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf
first_indexed	2023-09-18T23:00:10Z
last_indexed	2023-09-18T23:00:10Z
_version_	1777418120072265728

Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian

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