Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi

Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi

Cancer is a population of cells that characterized by over growth of antagonist cells. This research focused on the human myeoid leukemia (HL60) cell lines that derived from peripheral blood of woman with acute myelocytic leukemia. The main objectives of this study were to determine the interactive...

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Main Author: Mohd Nawi, Norfazlina
Format: Thesis
Language:English
Published: 2016
Online Access:http://ir.uitm.edu.my/id/eprint/17903/
http://ir.uitm.edu.my/id/eprint/17903/2/TM_NORFAZLINA%20MOHD%20NAWI%20AS%2016_5.pdf
id uitm-17903
recordtype eprints
spelling uitm-179032018-10-11T03:14:33Z http://ir.uitm.edu.my/id/eprint/17903/ Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi Mohd Nawi, Norfazlina Cancer is a population of cells that characterized by over growth of antagonist cells. This research focused on the human myeoid leukemia (HL60) cell lines that derived from peripheral blood of woman with acute myelocytic leukemia. The main objectives of this study were to determine the interactive effects of Nigella sativa and Zingiber zerumbet extracts on HL60 cells using the 3-[4,5 dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide (MTT) assay and to measure their mode of cell death using annexin-v flow cytometry assay. The cytotoxicity of hexane extract of Zingiber zerumbet rhizome towards HL60 cells was greater than the petroleum ether extract of Nigella sativa seed with IC50 values of 63.72±5.363|ug/ml and 654^g/ml. However, petroleum ether extract of Nigella sativa seed alone showed protective effect on normal V79 viable cells with higher IC50 (940|ig/ml) value but hexane extract of Zingiber zerumbet rhizome was toxic on V79 cells by lesser IC50 value (38|ng/ml). The combination treatment of petroleum ether extract of Nigella sativa seed and hexane extract of Zingiber zerumbet rhizome on both HL60 and V79 cells resulted the antagonist effects with CI value more than 1. Moreover, the mode of cell death showed that the petroleum ether extract of Nigella sativa seed alone conveyed the apoptosis and the hexane extract of Zingiber zerumbet rhizome regulated both apoptosis and necrosis cell death. As the HL60 cells exposed to the combination treatment of both plant extracts, the apoptosis cell increased and necrosis cells were decreased in the increased of combination dose. In conclusion, the combination between Nigella sativa and Zingiber zerumbet extracts conveyed the antagonist interactive effects on HL60 cells and indicated that Nigella sativa and Zingiber zerumbet could not be combined in order to achieve a safe drug. Moreover, combination of both plant extracts increased the apoptosis and reduced the necrosis of HL60 cells. 2016 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/17903/2/TM_NORFAZLINA%20MOHD%20NAWI%20AS%2016_5.pdf Mohd Nawi, Norfazlina (2016) Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi. Masters thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
description Cancer is a population of cells that characterized by over growth of antagonist cells. This research focused on the human myeoid leukemia (HL60) cell lines that derived from peripheral blood of woman with acute myelocytic leukemia. The main objectives of this study were to determine the interactive effects of Nigella sativa and Zingiber zerumbet extracts on HL60 cells using the 3-[4,5 dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide (MTT) assay and to measure their mode of cell death using annexin-v flow cytometry assay. The cytotoxicity of hexane extract of Zingiber zerumbet rhizome towards HL60 cells was greater than the petroleum ether extract of Nigella sativa seed with IC50 values of 63.72±5.363|ug/ml and 654^g/ml. However, petroleum ether extract of Nigella sativa seed alone showed protective effect on normal V79 viable cells with higher IC50 (940|ig/ml) value but hexane extract of Zingiber zerumbet rhizome was toxic on V79 cells by lesser IC50 value (38|ng/ml). The combination treatment of petroleum ether extract of Nigella sativa seed and hexane extract of Zingiber zerumbet rhizome on both HL60 and V79 cells resulted the antagonist effects with CI value more than 1. Moreover, the mode of cell death showed that the petroleum ether extract of Nigella sativa seed alone conveyed the apoptosis and the hexane extract of Zingiber zerumbet rhizome regulated both apoptosis and necrosis cell death. As the HL60 cells exposed to the combination treatment of both plant extracts, the apoptosis cell increased and necrosis cells were decreased in the increased of combination dose. In conclusion, the combination between Nigella sativa and Zingiber zerumbet extracts conveyed the antagonist interactive effects on HL60 cells and indicated that Nigella sativa and Zingiber zerumbet could not be combined in order to achieve a safe drug. Moreover, combination of both plant extracts increased the apoptosis and reduced the necrosis of HL60 cells.
format Thesis
author Mohd Nawi, Norfazlina
spellingShingle Mohd Nawi, Norfazlina
Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi
author_facet Mohd Nawi, Norfazlina
author_sort Mohd Nawi, Norfazlina
title Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi
title_short Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi
title_full Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi
title_fullStr Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi
title_full_unstemmed Cytotoxic effects and the action mechanism of single and combination treatments of Nigella sativa and Zingiber zerumbet on human myeloid leukemia (HL60) cell lines / Norfazlina Mohd Nawi
title_sort cytotoxic effects and the action mechanism of single and combination treatments of nigella sativa and zingiber zerumbet on human myeloid leukemia (hl60) cell lines / norfazlina mohd nawi
publishDate 2016
url http://ir.uitm.edu.my/id/eprint/17903/
http://ir.uitm.edu.my/id/eprint/17903/2/TM_NORFAZLINA%20MOHD%20NAWI%20AS%2016_5.pdf
first_indexed 2023-09-18T22:59:18Z
last_indexed 2023-09-18T22:59:18Z
_version_ 1777418065859837952

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