Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif

Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif

Leptin affects insulin secretion and action through either a central or peripheral mechanism. It increases glucose metabolism and energy expenditure. Though overweight and obese individuals have been reported to have high circulating leptin level, these effects of leptin are not evident. Exercise, o...

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Main Author: Mohd Sanif, Siti Nurr Atika
Format: Thesis
Language:English
Published: 2016
Subjects:
Organic substances
Online Access:http://ir.uitm.edu.my/id/eprint/16798/
http://ir.uitm.edu.my/id/eprint/16798/2/TM_SITI%20NURR%20ATIKA%20MOHD%20SANIF%20MD%2016_5.pdf
id uitm-16798
recordtype eprints
spelling uitm-167982018-10-05T02:38:08Z http://ir.uitm.edu.my/id/eprint/16798/ Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif Mohd Sanif, Siti Nurr Atika Organic substances Leptin affects insulin secretion and action through either a central or peripheral mechanism. It increases glucose metabolism and energy expenditure. Though overweight and obese individuals have been reported to have high circulating leptin level, these effects of leptin are not evident. Exercise, on the other hand, has been found to increase glucose uptake in these individuals. This study examined the effect of chronic leptin treatment and exercise on glucose homeostasis in Sprague-Dawley rats. Eight-week old rats were treated with either intraperitoneal injection of normal saline (Control; n=8), or leptin (60 |μg/kg body weight/day; Leptin; n=8), or leptin and exercise (60 | μg /kg body weight/day plus running on a treadmill every other day for 30 minutes at a speed of 30 m/min with 10° inclination; Leptin-exercise; n=8) or exercise only (running every other day for 30 minutes at a speed of 30 m/min with 10° inclination on a treadmill; Exercise; n=8) for six weeks. Following six weeks of treatment, glucose challenge was performed by intravenous infusion of 100 mg/ml of glucose for 5 minutes. Skeletal muscle tissues were collected at 0, 15, and 30 minutes respectively during the protocol for blood glucose, serum glucose, insulin, TRB3, GLUT4 protein and mRNA expression determination. Liver was harvested for TRB3 and GLUT2 protein and mRNA determination. Data were analyzed using One Way ANOVA with post-hoc analysis, and expressed as mean ± standard error of mean (SEM). Glucose clearance was delayed in the leptin group. This delay in glucose clearance might be associated with the significantly lower GLUT2 mRNA expression in the liver of leptin-treated rats. Leptin treatment also significantly reduced TRB3 mRNA levels in both muscle and liver tissues. More importantly, exercise reversed the leptin effects by promoting glucose clearance despite a significantly lower insulin peak, indicating a significant increase in insulin sensitivity. Exercise also significantly reduced TRB3 mRNA expression levels and increased GLUT4 mRNA levels in the muscle. In conclusion, six weeks of daily leptin administration resulted in delayed glucose clearance but concurrent exercise however prevented these effects of leptin by promoting glucose clearance and increasing insulin sensitivity. 2016 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/16798/2/TM_SITI%20NURR%20ATIKA%20MOHD%20SANIF%20MD%2016_5.pdf Mohd Sanif, Siti Nurr Atika (2016) Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif. Masters thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Organic substances
spellingShingle Organic substances
Mohd Sanif, Siti Nurr Atika
Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif
description Leptin affects insulin secretion and action through either a central or peripheral mechanism. It increases glucose metabolism and energy expenditure. Though overweight and obese individuals have been reported to have high circulating leptin level, these effects of leptin are not evident. Exercise, on the other hand, has been found to increase glucose uptake in these individuals. This study examined the effect of chronic leptin treatment and exercise on glucose homeostasis in Sprague-Dawley rats. Eight-week old rats were treated with either intraperitoneal injection of normal saline (Control; n=8), or leptin (60 |μg/kg body weight/day; Leptin; n=8), or leptin and exercise (60 | μg /kg body weight/day plus running on a treadmill every other day for 30 minutes at a speed of 30 m/min with 10° inclination; Leptin-exercise; n=8) or exercise only (running every other day for 30 minutes at a speed of 30 m/min with 10° inclination on a treadmill; Exercise; n=8) for six weeks. Following six weeks of treatment, glucose challenge was performed by intravenous infusion of 100 mg/ml of glucose for 5 minutes. Skeletal muscle tissues were collected at 0, 15, and 30 minutes respectively during the protocol for blood glucose, serum glucose, insulin, TRB3, GLUT4 protein and mRNA expression determination. Liver was harvested for TRB3 and GLUT2 protein and mRNA determination. Data were analyzed using One Way ANOVA with post-hoc analysis, and expressed as mean ± standard error of mean (SEM). Glucose clearance was delayed in the leptin group. This delay in glucose clearance might be associated with the significantly lower GLUT2 mRNA expression in the liver of leptin-treated rats. Leptin treatment also significantly reduced TRB3 mRNA levels in both muscle and liver tissues. More importantly, exercise reversed the leptin effects by promoting glucose clearance despite a significantly lower insulin peak, indicating a significant increase in insulin sensitivity. Exercise also significantly reduced TRB3 mRNA expression levels and increased GLUT4 mRNA levels in the muscle. In conclusion, six weeks of daily leptin administration resulted in delayed glucose clearance but concurrent exercise however prevented these effects of leptin by promoting glucose clearance and increasing insulin sensitivity.
format Thesis
author Mohd Sanif, Siti Nurr Atika
author_facet Mohd Sanif, Siti Nurr Atika
author_sort Mohd Sanif, Siti Nurr Atika
title Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif
title_short Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif
title_full Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif
title_fullStr Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif
title_full_unstemmed Effect of exercise on TRB3 and GLUT4 expression following glucose loading in chronic leptin treated rat / Siti Nurr Atika Mohd Sanif
title_sort effect of exercise on trb3 and glut4 expression following glucose loading in chronic leptin treated rat / siti nurr atika mohd sanif
publishDate 2016
url http://ir.uitm.edu.my/id/eprint/16798/
http://ir.uitm.edu.my/id/eprint/16798/2/TM_SITI%20NURR%20ATIKA%20MOHD%20SANIF%20MD%2016_5.pdf
first_indexed 2023-09-18T22:56:53Z
last_indexed 2023-09-18T22:56:53Z
_version_ 1777417913436733440

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