Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah

Oil palm fronds (OPF) are cheap, widespread and largely available throughout the year. OPF contains rich hemicellulose which can be hydrolysed by enzyme for production of xylooligosaccharides (XOs). The aim of this study was to produce high yield of XOs from OPF hemicellulose. XOs production was per...

Full description

Bibliographic Details
Main Author:	Mohamad Damanhuri Shah, Siti Normah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Biochemistry
Online Access:	http://ir.uitm.edu.my/id/eprint/15968/ http://ir.uitm.edu.my/id/eprint/15968/1/TM_SITI%20NORMAH%20MOHD%20DAMANHURI%20SHAH%20AS%2015_5.pdf

id	uitm-15968
recordtype	eprints
spelling	uitm-159682017-01-03T13:33:40Z http://ir.uitm.edu.my/id/eprint/15968/ Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah Mohamad Damanhuri Shah, Siti Normah Biochemistry Oil palm fronds (OPF) are cheap, widespread and largely available throughout the year. OPF contains rich hemicellulose which can be hydrolysed by enzyme for production of xylooligosaccharides (XOs). The aim of this study was to produce high yield of XOs from OPF hemicellulose. XOs production was performed by enzymatic hydrolysis of hemicellulose which was obtained by alkaline extraction from the OPF. OPF hemicellulose was hydrolysed using an endo-xylanse from Trichoderma longibrachiatum and the effects of different pH, temperature, enzyme and substrate concentration and hydrolysis time on the XOs yield were investigated. The hydrolysate containing XOs was then fractionated by ultrafiltration using 10 kDa, 3 kDa and 1 kDa molecular weight cut off (MWCO) membrane to purify the crude of XOs. The maximum yield of XOs (46%) was obtained at pH 4.6, temperature of 4°C, enzyme concentration of 2 U/ml and substrate concentration of 2% for 8 hours hydrolysis time with a considerable amount of xylose (12.55%). The percent recoveries of XOs in the final permeate of 3 kDa and 1 kDa MWCO membrane were 78.32% and 76.18%, respectively. The results showed that 3 kDa MWCO membrane fractionated XOs better than 1 kDa MWCO membrane with low amount of monosaccharides and high yield of xylobiose (X2) and xylotriose (X3). The results indicated the potential utilization of OPF for the production of XOs by enzymatic hydrolysis, which could be further extended as functional food ingredients. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/15968/1/TM_SITI%20NORMAH%20MOHD%20DAMANHURI%20SHAH%20AS%2015_5.pdf Mohamad Damanhuri Shah, Siti Normah (2015) Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah. Masters thesis, Universiti Teknologi MARA.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Biochemistry
spellingShingle	Biochemistry Mohamad Damanhuri Shah, Siti Normah Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
description	Oil palm fronds (OPF) are cheap, widespread and largely available throughout the year. OPF contains rich hemicellulose which can be hydrolysed by enzyme for production of xylooligosaccharides (XOs). The aim of this study was to produce high yield of XOs from OPF hemicellulose. XOs production was performed by enzymatic hydrolysis of hemicellulose which was obtained by alkaline extraction from the OPF. OPF hemicellulose was hydrolysed using an endo-xylanse from Trichoderma longibrachiatum and the effects of different pH, temperature, enzyme and substrate concentration and hydrolysis time on the XOs yield were investigated. The hydrolysate containing XOs was then fractionated by ultrafiltration using 10 kDa, 3 kDa and 1 kDa molecular weight cut off (MWCO) membrane to purify the crude of XOs. The maximum yield of XOs (46%) was obtained at pH 4.6, temperature of 4°C, enzyme concentration of 2 U/ml and substrate concentration of 2% for 8 hours hydrolysis time with a considerable amount of xylose (12.55%). The percent recoveries of XOs in the final permeate of 3 kDa and 1 kDa MWCO membrane were 78.32% and 76.18%, respectively. The results showed that 3 kDa MWCO membrane fractionated XOs better than 1 kDa MWCO membrane with low amount of monosaccharides and high yield of xylobiose (X2) and xylotriose (X3). The results indicated the potential utilization of OPF for the production of XOs by enzymatic hydrolysis, which could be further extended as functional food ingredients.
format	Thesis
author	Mohamad Damanhuri Shah, Siti Normah
author_facet	Mohamad Damanhuri Shah, Siti Normah
author_sort	Mohamad Damanhuri Shah, Siti Normah
title	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_short	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_full	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_fullStr	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_full_unstemmed	Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah
title_sort	production of xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / siti normah mohd damanhuri shah
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/15968/ http://ir.uitm.edu.my/id/eprint/15968/1/TM_SITI%20NORMAH%20MOHD%20DAMANHURI%20SHAH%20AS%2015_5.pdf
first_indexed	2023-09-18T22:55:03Z
last_indexed	2023-09-18T22:55:03Z
_version_	1777417798559989760

Production of Xylooligosaccharides from oil palm fronds hemicellulose by trichoderma longibra chia tum xylanase and ultrafiltration / Siti Normah Mohd Damanhuri Shah

Similar Items